Determination of the sulfhydryl content of thymus and liver using DPPH

‘Glucose 6-Phosphate-Dehydrogenase’ Activity and Thiol Content of Thymus Nuclei From Control and X-Irradiated Rats

“…The results suggested that alterations in the concentration of thiol (SH) compounds in the nuclei from normal rats might influence the activity of the dehydrogenase and that such alterations might be produced by X-irradiation. Glutathione and glutathione reductase (EC 1.6.4.2) have been described in nuclei from calf thymus (Stern & Timonen, 1954;Wang, 1962), and a study of nuclear thiol groups has been made by Klouwen (1962). The present paper indicates how the method of preparation of the nuclei affects the nature and amount of SR compounds found.…”

On the Composition of Human Myelin *

“…Sulphydryl groups were estimated by KLOUWEN'S (1962) method, which is applicable to total homogenates and measures absorption at a wave length where proteins do not interfere. Other substances, such as amines (PAPARIELIO and JANISH, 1965), also react with the diphenyl-picrylphenylhydrazine reagent but their contribution is controlled by KLOUWEN'S (1962) procedure.…”

Microdetermination of Protein-Sh and Low Molecular Sh-Groups Using Various Thiamine Disulfides as Reagent

“…Since protein-SH groups have various reactivities, it has been repeatedly stressed that no such method alone can draw a conclusion (1Q, 5, 11). Amperometric titration with AgNO3 and spectrophotometric titration with POMP are the representatives commonly used, but almighty (27,28) and new procedure and modification are still being investigated (21,(29)(30)(31).…”