Determination of the stability of tetracycline suspensions by high performance liquid chromatography

Grobben-Verpoorten, A; Dihuidi, K; Roets, Eugène; Hoogmartens, Jos; Vanderhaeghe, Hubert

doi:10.1007/bf01968711

Cited by 4 publications

(2 citation statements)

References 13 publications

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“…4-EATC, similar to lumitetracycline, a product of light-induced tetracycline metabolism, exhibits photosensitizing effects [ 40 , 47 ]. In weakly acidic and nearly neutral environments, tetracycline remains very stable and slightly reversibly epimerizes to 4-ETC, which is microbiologically inactive [ 43 , 48 , 49 ]. Therefore, it was important to obtain conditions that would not lead to drug degradation.…”

Section: Discussionmentioning

confidence: 99%

The Assessment of Physicochemical and Antimicrobial Properties of Hydrophilic Gels Containing Tetracycline Hydrochloride and Various Concentrations of Ethanol

Kostrzębska,

Junka,

Brożyna

et al. 2024

Pharmaceutics

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The high prevalence of acne, which affects nearly 85% of adolescents and young adults, underscores the importance of exploring new therapeutic solutions. The aim of the present study was to design a stable hydrogel formulation containing tetracycline hydrochloride (TC) in the presence of ethanol at various concentration levels. The antibiotic stability was assessed over a period of 84 days using the HPLC method. The rheological properties of the formulations and their microbiological activity were also evaluated. Hydrogels without ethanol and those containing 5% and 25% alcohol showed similar rheological properties and high stability of the antibiotic throughout the observation period. The formulation with the highest ethanol content of 50% differed significantly from the others in terms of rheological properties. Although the flow and viscosity curves were like those of the other formulations, the viscosity values were significantly lower. The stability of tetracycline in this formulation was also significantly lower, and by the 84th day of observation, the concentration of the drug had decreased to almost 45% of its initial content. The formulations containing the highest concentration of ethanol displayed the highest activity against the biofilm of the acne-causing agent, Cutibacterium acnes. The study demonstrated the possibility of developing stable and antimicrobial effective hydrogel formulations with tetracycline and ethanol as a substance enhancing drug penetration into the hair follicles.

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Section: Discussionmentioning

confidence: 99%

The Assessment of Physicochemical and Antimicrobial Properties of Hydrophilic Gels Containing Tetracycline Hydrochloride and Various Concentrations of Ethanol

Kostrzębska,

Junka,

Brożyna

et al. 2024

Pharmaceutics

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“…The pH value of solutions A1, A2 and A3 due to dissolved TC oscillated around 2.998. At such low pH values, the leading phenomenon in TC transformations is epimerization, which occurs most quickly at pH values 3–5 or, according to some other researchers, 2–6, while in solutions with a pH value below 2, the main decomposition product is ATC [ 7 , 32 , 33 , 34 ]. The pH values of hydrogels were about 6.65, which could significantly slow down the processes of TC molecule decomposition.…”

Section: Discussionmentioning

confidence: 99%

Evaluation of the Influence of a Hydrogel Containing AMPD on the Stability of Tetracycline Hydrochloride

2021

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Tetracyclines, as beneficial antimicrobial factors in both local and systemic therapy, are characterized by high instability. The aim of the study was the development of the influence of hydrogel formulation on the tetracycline hydrochloride (TC) level under varying storage conditions. The HPLC, XPRD as well as SEM and macroscopic observations were involved in the study. The TC concentration decreased within ca. two months from 9.37 µg/mL to 4.41 µg/mL in the case of the photoprotected TC solution stored at 23 °C, whereas the decrease in storage temperature did not improve the final level of TC. In the presence of AMPD, the TC level in aqueous solution decreased drastically to ca. 1 µg/mL. Application of a polyacrylic acid derivative enabled conservation of the TC level through the ca. two months. Thus, the use of alcoholamine in the preparation of the TC hydrogel may result in the development of a therapeutic product with a dual action against acne, including antimicrobial activity and saponification of free fatty acids deposited in the follicles.

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Separation of tetracycline related substance by high-performance liquid chromatography on poly(styrene—divinylbenzene)

Wolfs

Roets²,

Hoogmartens³

et al. 1986

Journal of Chromatography A

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Determination of the stability of tetracycline suspensions by high performance liquid chromatography

Cited by 4 publications

References 13 publications

The Assessment of Physicochemical and Antimicrobial Properties of Hydrophilic Gels Containing Tetracycline Hydrochloride and Various Concentrations of Ethanol

The Assessment of Physicochemical and Antimicrobial Properties of Hydrophilic Gels Containing Tetracycline Hydrochloride and Various Concentrations of Ethanol

Evaluation of the Influence of a Hydrogel Containing AMPD on the Stability of Tetracycline Hydrochloride

Separation of tetracycline related substance by high-performance liquid chromatography on poly(styrene—divinylbenzene)

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