Determination of the six major human herpesviruses in cerebrospinal fluid and blood specimens of children

Dong, Guanping; Shang, Shi Qiang; Liang, Li; Yu, X.

doi:10.1080/08035250410022477

Cited by 5 publications

(2 citation statements)

References 19 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Secondly, revealed by PCR, CMV DNA presence in the samples is not always accompanied by the presence of serum specific antibodies. This situation is typical in particular for the infants with immature immune system (Dong et al, 2004 , 2005 ).…”

Section: Coexistence Of Certain Infections and Cd: A Critical Look Atmentioning

confidence: 96%

Microbes and Viruses Are Bugging the Gut in Celiac Disease. Are They Friends or Foes?

Lerner

Arleevskaya

Schmiedl³

et al. 2017

Front. Microbiol.

View full text Add to dashboard Cite

The links between microorganisms/viruses and autoimmunity are complex and multidirectional. A huge number of studies demonstrated the triggering impact of microbes and viruses as the major environmental factors on the autoimmune and inflammatory diseases. However, growing evidences suggest that infectious agents can also play a protective role or even abrogate these processes. This protective crosstalk between microbes/viruses and us might represent a mutual beneficial equilibrium relationship between two cohabiting ecosystems. The protective pathways might involve post-translational modification of proteins, decreased intestinal permeability, Th1 to Th2 immune shift, induction of apoptosis, auto-aggressive cells relocation from the target organ, immunosuppressive extracellular vesicles and down regulation of auto-reactive cells by the microbial derived proteins. Our analysis demonstrates that the interaction of the microorganisms/viruses and celiac disease (CD) is always a set of multidirectional processes. A deeper inquiry into the CD interplay with Herpes viruses and Helicobacter pylori demonstrates that the role of these infections, suggested to be potential CD protectors, is not as controversial as for the other infectious agents. The outcome of these interactions might be due to a balance between these multidirectional processes.

show abstract

Section: Coexistence Of Certain Infections and Cd: A Critical Look Atmentioning

confidence: 96%

Microbes and Viruses Are Bugging the Gut in Celiac Disease. Are They Friends or Foes?

Lerner

Arleevskaya

Schmiedl³

et al. 2017

Front. Microbiol.

View full text Add to dashboard Cite

show abstract

“…Multiplex PCR, in which two or more target sequences are amplified simultaneously in one single reaction, is highly cost-effective and efficient. However, further species identification of PCR products remains a tedious problem involving Southern Blot or restriction fragment length polymorphism (RPLF) [Hudnall et al, 2004;Dong et al, 2005;Tafreshi et al, 2005]. DNA microarrays, due to its capacity to accommodate different specific probes, offer an ideal way for species identification of PCR products .…”

Section: Introductionmentioning

confidence: 99%

DNA microarray technology for simultaneous detection and species identification of seven human herpes viruses

Zheng

et al. 2008

Journal of Medical Virology

View full text Add to dashboard Cite

The aim of the study was to develop a multiplex PCR-based DNA microarray technology for simultaneous detection and species identification of seven human herpes viruses, namely herpes simplex virus type 1, type 2 (HSV-1, HSV-2), varicella-zoster virus (VZV), Epstein-Barr virus (EBV), cytomegalovirus (CMV), and human herpes virus 6 (HHV-6A, HHV-6B), and to apply this technology to accurate diagnosis of herpesvirus-associated diseases. Primers and oligonucleotide probes were designed and synthesized based on the highly conserved regions of the DNA polymerase gene in human herpes viruses. DNA microarrays were made by printing the oligonucleotide probes onto special glass slides. After amplification and labeling with CY5, the PCR products were hybridized with the DNA microarrays and species identified. Sixty-one cerebrospinal fluid (CSF) and 132 blood specimens were analyzed by this technique, and the results were compared with those of TaqMan PCR. Several specimens were sequenced further after cloning. The PCR products of the seven human herpes viruses ranged from 224 to 252 bp, and could be species identified with DNA microarrays. The detection limits were 10(1) copies/microl for each virus. And the test showed no cross-reaction to DNA extracted from S. aureus, E. coli, hepatitis B virus, Cryptococcus neoformans, Candida albicans and human genome. Among 132 blood and 61 CSF specimens, 55 were tested positive for human herpes virus DNA. Compared with the results of TaqMan PCR, the sensitivity and specificity of the DNA microarray technology was 96.2% and 99.3%, respectively. This multiplex PCR-based DNA microarray technology, which is rapid, specific and sensitive, serves as an effective technique for simultaneous detection and species identification of seven human herpes viruses.

show abstract

Rapid diagnosis of herpetic encephalitis in children by PCR-microarray technology for simultaneous detection of seven human herpes viruses

Shi

Cai

et al. 2009

Eur J Pediatr

View full text Add to dashboard Cite

The aim of the study was to evaluate retrospectively the usefulness of polymerase chain reaction (PCR)-microarray technology, which can simultaneously detect seven human herpes viruses for rapid and accurate diagnosis of herpetic encephalitis in children. We simultaneously amplified herpes simplex virus type 1 and type 2 (HSV-1 and HSV-2); varicella-zoster virus; Epstein-Barr virus (EBV); cytomegalovirus (CMV); and human herpes virus 6 (HHV-6A and HHV-6B) by multiplex PCR, and genotyped by DNA microarray technology. The multiplex primers and oligonucleotide probes were designed and synthesized based on the highly conserved regions of the DNA polymerase gene in human herpes viruses. Two hundred ninety cerebrospinal fluid (CSF) specimens from children with clinical suspicion of viral encephalitis were screened by PCR-microarray technology. The results were compared with those of TaqMan PCR kits of common herpes virus. The PCR-microarray technology could detect as few as 10 copies of viral loads. There was no nonspecific hybridizing signal between probes and no cross-reaction to DNA extracted from the pathogens we used. Of 290 cases, 11 were tested positive by PCR-microarray technology. Among them, three were positive for HSV-1, two were positive for HSV-2, one was positive for EBV, two were positive for CMV, two were positive for HHV-6A, one was positive for HHV-6B, and one showed mixed infection of HSV-2 and CMV, and the positive rate was 3.8%. Compared with the results of TaqMan PCR, the sensitivity of PCR-microarray technology was 91.7%, the specificity was 100%, and the index of accurate diagnosis was 0.917. None of the 30 control CSF specimens was tested positive in both methods. Our study suggests that the simultaneous detection of seven human herpes viruses by PCR-microarray technology is the method of choice for rapid, accurate, and specific etiological diagnosis of herpetic encephalitis in children.

show abstract

Determination of the six major human herpesviruses in cerebrospinal fluid and blood specimens of children

Abstract: PCR-RFLP is a specific, sensitive and accurate technique for the identification of herpesvirus infections in the CSF and blood of children.

Cited by 5 publications

References 19 publications

Microbes and Viruses Are Bugging the Gut in Celiac Disease. Are They Friends or Foes?

Microbes and Viruses Are Bugging the Gut in Celiac Disease. Are They Friends or Foes?

DNA microarray technology for simultaneous detection and species identification of seven human herpes viruses

Rapid diagnosis of herpetic encephalitis in children by PCR-microarray technology for simultaneous detection of seven human herpes viruses

Contact Info

Product

Resources

About