Determination of the Severity of Ureteropelvic Junction Obstruction Using Urinary Epidermal Growth Factor and Kidney Injury Molecule 1 levels

Mohammadjafari, Hamid; Rafiei, Alireza; Kosaryan, Mehrnoush; Yeganeh, Yalda; Hosseinimehr, Seyed Jalal

doi:10.2217/bmm.14.79

Cited by 16 publications

(11 citation statements)

References 17 publications

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“…Out of the 38 studies included in this systematic review, all (100%) searched for urine biomarkers and 5 (13.2%) (7,12,18,22,34), included also serum samples. Thirty-two (84.2%) studies considered only patients with unilateral PUJO while 6 (15.8%) included also patients with bilateral obstruction (5,6,20,21,33,35). The quantitative urine protein analysis was performed using commercially available human sandwich enzyme-linked immunosorbent assay (ELISA) kits in 32 (84.2%) studies.…”

Section: Resultsmentioning

confidence: 99%

“…Fourteen (36.8%) studies compared patients undergoing pyeloplasty [surgical group (SG)] with patients with mild non-obstructive dilatation not requiring surgery [non-surgical group (NSG)] and healthy children [reference group (RG)] (9,10, [14][15][16][17][18]20,22,27,29,[31][32][33]. Seven (18.4%) articles analysed urine biomarker concentrations in patients undergoing surgery (SG) and related them with patients conservatively treated (NSG) (6,19,21,28,36,37,41). Thirteen (34.2%) studies reported the comparison between surgical patients (SG) and healthy subjects (RG) (8,11,13,(24)(25)(26)30,34,35,(38)(39)(40)42).…”

Section: Resultsmentioning

confidence: 99%

“…Twelve (30.8%) studies investigated the correlation between preoperative biomarker concentration with the anterior posterior renal pelvis diameter (DAP) (9,12,14,16,18,22,23,25,27,(30)(31)(32) while 20 (52.6%) studies investigated the correlation between preoperative biomarker concentration with the split renal function (SRF) evaluated on nuclear medicine assessments (8,9,(12)(13)(14)16,18,19,22,(25)(26)(27)(28)(29)(30)(31)(32)39,41,42). ROC curves were used to investigate the performance of urinary biomarkers in the total patient data set in 21 (55.3%) studies (5)(6)(7)(8)(9)(14)(15)(16)(17)(18)(19)(20)(21)(22)24,(27)…”

Section: Resultsmentioning

confidence: 99%

See 2 more Smart Citations

Urinary biomarkers in pelvic-ureteric junction obstruction: a systematic review

Paraboschi¹,

Mantica²,

Dalton³

et al. 2020

Transl Androl Urol

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Antenatal hydronephrosis is a common finding detected on prenatal ultrasound. Although hydronephrosis will spontaneously resolve in the majority of newborns, there is a significant amount of cases that will worsen with the risk of a progressive and permanent loss of renal function. There is an increasing concern among experts that the current criteria for evaluation of clinically significant obstructions are limited.Our aim is to provide a systematic review of the available literature on biomarkers of renal injury, potential targets for diagnosis and prognosis of children with hydronephrosis. The main search was conducted in the electronic databases from inception through March 2019 using various combinations of the keywords: pelvicureteric [All Fields] AND junction [All Fields] AND obstruction [All Fields] AND "biomarkers" [MeSH Terms] OR "biomarkers" [All Fields] OR "biomarker" [All Fields]. To broaden the research, additional articles were identified through hand-searching review of the references reported in each study previously selected.Histopathological studies, studies with no control group or with participants suffering from concomitant urological diseases and articles published in language other than English were excluded. Data on study design, sample size, average patient age, hydronephrosis definition used, surgical indication, duration and pattern of follow-up, details on biomarker studied, diagnostic test characteristics, area under the curve (AUC) on receiver operating characteristic (ROC) analysis with the best cut-off (BCO) values, sensitivity, specificity and outcomes were all collected. 38 articles analysing 41 biomarkers were selected. The most frequent proteins investigated were neutrophil gelatinase-associated lipocalin (NGAL) (n=9; 23.7%), monocyte chemotactic peptide-1 (MCP1) (n=8; 21.1%), transforming growth factor β1 (TGFβ1) (n=7; 18.4%), epidermal growth factor (EGF) (n=6; 15.8%) and kidney injury molecule 1 (KIM 1) (n=6; 15.8%). Twenty-seven (71.1%) studies evaluated the effect of pyeloplasty on voided urine biomarker concentrations, comparing their values before and after surgery. Twelve (31.6%) studies investigated the correlation between preoperative biomarker concentration and the anterior posterior renal pelvis diameter (DAP) while 20 (52.6%) studies investigated the correlation between preoperative biomarker concentration with the split renal function (SRF) measured on nuclear medicine assessments. ROC curves were used to investigate the performance of urinary biomarkers in the total patient data set in 27 (71.1%) studies. Some biomarkers offer promising results. However, a critic analysis of the published studies demonstrates bias and lack of consistency suggesting that larger multicentre and carefully designed prospective studies are still needed to evaluate the clinical usefulness of urinary biomarkers in the diagnosis and follow-up of children with congenital obstructive hydronephrosis.

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Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

See 1 more Smart Citation

Urinary biomarkers in pelvic-ureteric junction obstruction: a systematic review

Paraboschi¹,

Mantica²,

Dalton³

et al. 2020

Transl Androl Urol

View full text Add to dashboard Cite

show abstract

“…The usefulness of urinary KIM-1 excretion as a new early biomarker of ON in patients with HN was reported by several recent studies [ 21 , 22 , 25 , 29 , 30 ]. However, in the study by Noyan et al [ 20 ], hydronephrotic children with kidney dysfunction were not characterized by increased urinary KIM-1 excretion.…”

Section: Discussionmentioning

confidence: 96%

Potential Novel Biomarkers of Obstructive Nephropathy in Children with Hydronephrosis

Bieniaś

Sikora

2018

Disease Markers

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Obstructive nephropathy (ON) secondary to the congenital hydronephrosis (HN) is one of the most common causes of chronic kidney disease in children. Neither currently used imaging techniques nor conventional laboratory parameters are sufficient to assess the onset and outcome of this condition; hence, there is a need to prove the usefulness of newly discovered biomarkers of kidney injury in this respect. The purpose of the study was to assess the urinary excretion of alpha-GST, pi-GST, NGAL, and KIM-1 and the serum level of NGAL in children with congenital unilateral hydronephrosis secondary to ureteropelvic junction obstruction. The results were evaluated in relation to severity of HN, the presence of ON, relative function of an obstructed kidney, and the presence of proteinuria. The study comprised 45 children with HN of different grades and 21 healthy controls. Urinary and serum concentrations of biomarkers were measured using specific ELISA kits. Urinary biomarker excretions were expressed as a biomarker/creatinine (Cr) ratio. Patients with the highest grades of HN showed significantly increased values of all measured biomarkers, whereas those with the lowest grades of HN displayed only significant elevation of urinary alpha-GST and the serum NGAL. Urinary NGAL positively correlated with percentage loss of relative function of an obstructed kidney in renal scintigraphy. In patients with proteinuria, significantly higher urinary alpha-GST excretion was revealed as compared to those without this symptom. The ROC curve analysis showed the best diagnostic profile for urinary alpha-GST/Cr and NGAL/Cr ratios in the detection of ON. In conclusion, the results of the study showed that urinary alpha-GST and NGAL are promising biomarkers of ON. Ambiguous results of the remaining biomarkers, i.e., urinary pi-GST and KIM-1, and serum NGAL level may be related to a relatively small study group. Their utility in an early diagnosis of ON should be reevaluated.

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“…Immortalized human renal tubular epithelial cells (HKC) were cultured at 37 °C with humidified atmosphere of 5 % CO 2 in DMEM/F12 supplemented with 10 % fetal bovine serum (FBS) and 2 ng/mL mouse epidermal growth factor and were seeded into 96-well culture plates (Corning, Schiphol-Rijk, The Netherlands) for 24 h. Subconfluent cells (culture surface covering nearly 70 %) were then exposed to various AMB or AMB formulation concentrations (1.0–10.0 μM). As kidney injury molecule-1 (KIM-1) and type I transmembrane glycoprotein neutrophil gelatinase-associated lipocalin (NGAL) have been reported to correlate with proximal tubular injury [17, 18], the cytotoxicity was measured periodically by measuring KIM-1 and NGAL using immunofluorescence, WB, and RT-PCR methods to determine early damage caused by exposure to AMB and AMB formulations. The prime set for NGAL was forward, 5′-TCA CCT CCG TCC TGT TTA G-3′ and reverse, 5′-CTC CTT GGT TCT CCC GTA-3′, and that for KIM-1 was forward, 5′-ACTCCTGCAGACTGGAATGG-3′ and reverse, 5′-CAAAGCTCAGAGAGCCCATC-3′.…”

Section: Methodsmentioning

confidence: 99%

Enhanced Antifungal Activity by Ab-Modified Amphotericin B-Loaded Nanoparticles Using a pH-Responsive Block Copolymer

et al. 2015

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Fungal infections are an important cause of morbidity and mortality in immunocompromised patients. Amphotericin B (AMB), with broad-spectrum antifungal activity, has long been recognized as a powerful fungicidal drug, but its clinical toxicities mainly nephrotoxicity and poor solubility limit its wide application in clinical practice. The fungal metabolism along with the host immune response usually generates acidity at sites of infection, resulting in loss of AMB activity in a pH-dependent manner. Herein, we developed pH-responsive AMB-loaded and surface charge-switching poly(d,l-lactic-co-glycolic acid)-b-poly(l-histidine)-b-poly(ethylene glycol) (PLGA-PLH-PEG) nanoparticles for resolving the localized acidity problem and enhance the antifungal efficacy of AMB. Moreover, we modified AMB-encapsulated PLGA-PLH-PEG nanoparticles with anti-Candida albicans antibody (CDA) (CDA-AMB-NPs) to increase the targetability. Then, CDA-AMB-NPs were characterized in terms of physical characteristics, in vitro drug release, stability, drug encapsulation efficiency, and toxicity. Finally, the targetability and antifungal activity of CDA-AMB-NPs were investigated in vitro/in vivo. The result demonstrated that CDA-AMB-NPs significantly improve the targetability and bioavailability of AMB and thus improve its antifungal activity and reduce its toxicity. These NPs may become a good drug carrier for antifungal treatment.

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Determination of the Severity of Ureteropelvic Junction Obstruction Using Urinary Epidermal Growth Factor and Kidney Injury Molecule 1 levels

Abstract: Evaluation of the urinary KIM-1:Cr ratio may help identify neonates with severe obstructive hydronephrosis.

Cited by 16 publications

References 17 publications

Urinary biomarkers in pelvic-ureteric junction obstruction: a systematic review

Urinary biomarkers in pelvic-ureteric junction obstruction: a systematic review

Potential Novel Biomarkers of Obstructive Nephropathy in Children with Hydronephrosis

Enhanced Antifungal Activity by Ab-Modified Amphotericin B-Loaded Nanoparticles Using a pH-Responsive Block Copolymer

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