Determination of the S(+)- and R(−)-enantiomers of baclofen in plasma and urine by gas chromatography using a chiral fused-silica capillary column and an electron-capture detector

Sioufi, A.; Kaiser, Guenther; Leroux, Fanny; Dubois, J.P.

doi:10.1016/s0021-9673(01)83910-8

Cited by 33 publications

(16 citation statements)

References 11 publications

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“…Many efforts have been devoted to the development of analytical methods for the determination of BAL using spectrophotometry [3,4], potentiometry [5], thin-layer chromatography [6], gas chromatography [7], high-performance liquid chromatography (HPLC) [8,9,10] and capillary electrophoresis [11,12]. As the target analyte is usually present in complex samples at low concentrations, HPLC coupled with all kinds of detection methods such as UV-vis absorption [8,9], fluorescence detection [10] and mass spectrometry (MS) [13] is an especially effective and important technique due to its separation process and high selectivity.…”

Section: Introductionmentioning

confidence: 99%

Rapid and sensitive analysis of baclofen by high-performance liquid chromatography with UV-Vis and FD detection

Cao

2012

Acta Chromatographica

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Summary. Two rapid, sensitive and reproducible methods for the determination of baclofen(BAL) in urine and plasma based on high-performance liquid chromatography (HPLC) with UV-vis and fluorescent detection, respectively, were developed for the first time using a new synthesized fluorescent label, 6-oxy-(N-succinimidylacetate)-9-(2'-methoxycarbonyl) fluorescein (SAMF). The optimal derivatization yield was achieved in borate buffer (pH 8.0) for 15 min at room temperature (25 °C). With a mixture of methanol and water containing 5 mmol L −1 sodium citrate buffer (pH 5.0) as mobile phase, BAL was determined at λ = 455 nm with UV-vis detection and at λ ex /λ em = 488/520 nm with FD detection. The detection limits are 1.065 × 10 −3 mg mL −1 and 1.065 × 10 −2 mg mL −1 with HPLC-UV-vis and HPLC-FD, respectively. The proposed method has been successfully applied to the analysis of BAL in human urine and plasma samples. The established method is rapid (15 min of derivatization process and 10 min of chromatographic run), reproducible and sensitive.

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Section: Introductionmentioning

confidence: 99%

Rapid and sensitive analysis of baclofen by high-performance liquid chromatography with UV-Vis and FD detection

Cao

2012

Acta Chromatographica

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“…All these methods were only used for the determination of baclofen racemate. For chiral analysis, different methods were developed such as GC with electron capture detection [26,27] as well as HPLC coupled with fluorescence [28,29], UV [30,31], and MS detection [32]. As reported in the literature, there are only few papers dedicated to the chiral separation of baclofen by CE with derivatized CDs [33] and crown ethers [34,35] in acidic media with UV detection.…”

Section: Introductionmentioning

confidence: 99%

Enantioseparation of baclofen with highly sulfated β‐cyclodextrin by capillary electrophoresis with laser‐induced fluorescence detection

Kavran-Belin

Rudaz

Veuthey

2005

J of Separation Science

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Enantioseparation of baclofen with highly sulfated b-cyclodextrin by capillary electrophoresis with laser-induced fluorescence detectionThe enantioseparation of baclofen (4-amino-3-p-chlorophenylbutyric acid) was achieved by CE-LIF with highly sulfated b-CD (HS-b-CD) as chiral selector. Naphthalene-2,3-dicarboxaldehyde was used for the derivatization of nonfluorescent baclofen. HS-b-CD (2%) containing 50 mM borate buffer at pH 9.5 was chosen as the optimal running electrolyte and applied to the analysis of baclofen enantiomers in human plasma. The linearity of calibration curves (R 2 F 0.998) for R-( -) and S-(+)-baclofen was in the 0.1-2.0 lM concentration range. After a simple ACN-protein precipitation, the LOD of baclofen in plasma sample was found as low as 50 nM.

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“…So far few analytical methods have been developed to determine each baclofen enantiomer in biologic material [8][9][10][11]. Those methods that do exist require troublesome extraction and derivatization procedures for sample cleanup, enantioselective separation, or sensitive fluorescence detection.…”

Section: Introductionmentioning

confidence: 99%

Simple and sensitive liquid chromatography–tandem mass spectrometry method for determination of the S(+)- and R(−)-enantiomers of baclofen in human plasma and cerebrospinal fluid

Goda¹,

Murayama²,

Fujimaki³

et al. 2004

Journal of Chromatography B

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Determination of the S(+)- and R(−)-enantiomers of baclofen in plasma and urine by gas chromatography using a chiral fused-silica capillary column and an electron-capture detector

Cited by 33 publications

References 11 publications

Rapid and sensitive analysis of baclofen by high-performance liquid chromatography with UV-Vis and FD detection

Rapid and sensitive analysis of baclofen by high-performance liquid chromatography with UV-Vis and FD detection

Enantioseparation of baclofen with highly sulfated β‐cyclodextrin by capillary electrophoresis with laser‐induced fluorescence detection

Simple and sensitive liquid chromatography–tandem mass spectrometry method for determination of the S(+)- and R(−)-enantiomers of baclofen in human plasma and cerebrospinal fluid

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