Determination of the Role of Microcystis aeruginosa in Toxin Generation Based on Phosphoproteomic Profiles

Qu, Jiangqi; Shen, Liping; Zhao, Meng; Li, Wentong; Jia, Chengxia; Zhu, Hua; Zhang, Qingjing

doi:10.3390/toxins10070304

Cited by 23 publications

(12 citation statements)

References 50 publications

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“…In Microcystis aeruginosa , a positive relationship between the microcystin content of cells and their specific growth rate was reported 56 . Additionally, several other physiological functions have been suggested for cyanotoxins (including microcystin), such as photosynthesis efficiency, carbohydrate metabolism, cell signaling, nutrient uptake, iron scavenging, maintenance of homeostasis, and protection against oxidative stress 22 , 57 – 61 .…”

Section: Discussionmentioning

confidence: 99%

Competitive interactions as a mechanism for chemical diversity maintenance in Nodularia spumigena

Lage

Mazur‐Marzec

Gorokhova

2021

Sci Rep

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Nodularia spumigena is a bloom-forming diazotrophic cyanobacterium inhabiting brackish waters worldwide. This species produces non-ribosomal peptides (NRPs), including the hepatotoxin nodularin, often referred to as cyanotoxin. Several known classes of NRPs have various biological activities, although their modes of action are poorly understood. In the Baltic N. spumigena, there is a high NRP chemodiversity among strains, allowing their grouping in specific chemotypes and subgroups. Therefore, it is relevant to ask whether the NRP production is affected by intraspecific interactions between the co-existing strains. Using a novel approach that combines culture technique and liquid chromatography-tandem mass spectrometry for the NRP analysis, we examined N. spumigena strains under mono- and co-culture conditions. The test strains were selected to represent N. spumigena belonging to the same or different chemotype subgroups. In this setup, we observed physiological and metabolic responses in the test strains grown without cell contact. The changes in NRP levels to co-culture conditions were conserved within a chemotype subgroup but different between the subgroups. Our results suggest that intraspecific interactions may promote a chemical diversity in N. spumigena population, with higher NRP production compared to a single-strain population. Studying allelochemical signalling in this cyanobacterium is crucial for understanding toxicity mechanisms and plankton community interactions in the Baltic Sea and other aquatic systems experiencing regular blooms.

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Section: Discussionmentioning

confidence: 99%

Competitive interactions as a mechanism for chemical diversity maintenance in Nodularia spumigena

Lage

Mazur‐Marzec

Gorokhova

2021

Sci Rep

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“…MC-LR is very stable and resistant to many natural factors including extreme pH, high temperature, and sunlight in the environment owing to its cyclic structure [ 3 , 6 , 14 ]. Moreover, MC-LR can be accumulated in aquatic organisms and food crops representing a health hazard to humans and animals through food chains [ 10 , 15 , 16 ]. It is very important to reduce MC-LR concentrations in freshwater ecosystem.…”

Section: Introductionmentioning

confidence: 99%

Removal of Microcystin-LR by a Novel Native Effective Bacterial Community Designated as YFMCD4 Isolated from Lake Taihu

Yang

Guo

Huang

et al. 2018

Toxins

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Microcystin-LR (MC-LR) is the most toxic and frequently detected monocyclic heptapeptide hepatotoxin produced by cyanobacteria, which poses a great threat to the natural ecosystem and public health. It is very important to seek environment-friendly and cost-efficient methods to remove MC-LR in water. In this study, the MC-degrading capacities of a novel indigenous bacterial community designated as YFMCD4 and the influence of environmental factors including various temperatures, MC concentrations and pH on the MC-degrading activities were investigated utilizing high-performance liquid chromatography (HPLC). In addition, the MC-degrading mechanism of YFMCD4 was also studied using HPLC coupled with a mass spectrometry equipped with electrospray ionization interface (HPLC-ESI-MS). The data showed MC-LR was completely removed at the maximum rate of 0.5 µg/(mL·h) under the optimal condition by YFMCD4. Two pure bacterial strains Alcaligenes faecalis and Stenotrophomonas acidaminiohila were isolated from YFMCD4 degraded MC-LR at a slower rate. The MC-degrading rates of YFMCD4 were significantly affected by different temperatures, pH and MC-LR concentrations. Two intermediates of a tetrapeptide and Adda appeared in the degradation process. These results illustrate that the novel YFMCD4 is one of the highest effective MC-degrading bacterial community, which can completely remove MC-LR and possesses a significant potential to treat water bodies contaminated by MC-LR.

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“…M. aeruginosa is a photosynthetic cyanobacterium that plays an important role in global oxygenation; also, it forms water blooms in nutrient rich waters, causing water contamination and public health threats ( Qu et al., 2018 ). According to Fig.…”

Section: Infectious Agents Commonly Found In Water and Wastewatermentioning

confidence: 99%

Photo-assisted electrochemical advanced oxidation processes for the disinfection of aqueous solutions: A review

et al. 2021

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Disinfection is usually the final step in water treatment and its effectiveness is of paramount importance in ensuring public health. Chlorination, ultraviolet (UV) irradiation and ozone (O 3 ) are currently the most common methods for water disinfection; however, the generation of toxic by-products and the non-remnant effect of UV and O 3 still constitute major drawbacks. Photo-assisted electrochemical advanced oxidation processes (EAOPs) on the other hand, appear as a potentially effective option for water disinfection. In these processes, the synergism between electrochemically produced active species and photo-generated radicals, improve their performance when compared with the corresponding separate processes and with other physical or chemical approaches. In photo-assisted EAOPs the inactivation of pathogens takes place by means of mechanisms that occur at different distances from the anode, that is: (i) directly at the electrode’s surface (direct oxidation), (ii) at the anode’s vicinity by means of electrochemically generated hydroxyl radical species (quasi-direct), (iii) or at the bulk solution (away from the electrode surface) by photo-electrogenerated active species (indirect oxidation). This review addresses state of the art reports concerning the inactivation of pathogens in water by means of photo-assisted EAOPs such as photo-electrocatalytic process, photo-assisted electrochemical oxidation, photo-electrocoagulation and cathodic processes. By focusing on the oxidation mechanism, it was found that while quasi-direct oxidation is the preponderant inactivation mechanism, the photo-electrocatalytic process using semiconductor materials is the most studied method as revealed by numerous reports in the literature. Advantages, disadvantages, trends and perspectives for water disinfection in photo-assisted EAOPs are also analyzed in this work.

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Determination of the Role of Microcystis aeruginosa in Toxin Generation Based on Phosphoproteomic Profiles

Cited by 23 publications

References 50 publications

Competitive interactions as a mechanism for chemical diversity maintenance in Nodularia spumigena

Competitive interactions as a mechanism for chemical diversity maintenance in Nodularia spumigena

Removal of Microcystin-LR by a Novel Native Effective Bacterial Community Designated as YFMCD4 Isolated from Lake Taihu

Photo-assisted electrochemical advanced oxidation processes for the disinfection of aqueous solutions: A review

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