Determination of the Relative Spectral Sensitivity of Phototubes*

Moran, H.S.

doi:10.1364/josa.45.000012

Cited by 10 publications

(3 citation statements)

References 2 publications

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“…(27) describe the application of a Luminous Standard Lamp, calibrated in lumens. Some other workers have calibrated their phototubes using a lamp of known color temperature and a single monochromator (43) and others with interference filters (29) in the style of Method D. A C14 labelled scintillator solution has recently been calibrated for total photon output and is being made available for workers in the field (30) and T o t t e P ) has used one of these light emission standards to determine the quantum yield of dimethyl-biacridylium ion chemiluminescence.…”

Section: Sources Of Errormentioning

confidence: 99%

Absolute Spectral Sensitivity of Phototubes and the Application to the Measurement of the Absolute Quantum Yields of Chemiluminescence and Bioluminescence*

Lee

Seliger

1965

Photochem & Photobiology

148

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Methods forthe absolute measurement of thespectral sensitivity of phototubes are reviewed in detail. Several experimental arrangements are described for the independent determination of these values to insure the elimination of systematic error. On the basis of these measurements a rapid, simple technique is described for measuring the average absolute spectral sensitivity of a phototube over a defined emission spectrum, thus obviating the original tedious point-by-point methods and allowing frequent re-Standardization. The application of these standardized phototubes to the measurement of the absolute quantum yields of the chemiluminescence of luminol and the bioluminescence of luminous bacteria and the firefly is described. An absolute low-level light emission standard in the form of the luminol chemiluminescence reaction, either in aqueous or in the non-aqueous solvent dimethylsulfoxide is proposed for general use and the conditions for its use are described in detail. It should therefore be possible for any researcher to obtain an absolute calibration, in his own experimental geometry, in the blue region of the spectrum. I N T R O D U C T I O NIN THE elucidation of the molecular mechanisms of chemiluminescence and bioluminescence and for both military and commercial applications of chemiluminescent reactions, it is extremely important to know the quantum yield; that is the number of light quanta emitted per substrate molecule reacting. In general this requires the determination, aside from the chemical stoichiometry, of the emission spectrum of the light reaction, the absolute spectral photon sensitivity of the phototube used and the physical geometry of the experiment. Seliger and McElroy'l) described a measurement of the absolute spectral sensitivity of a phototube by a standard thermopile substitution technique with an overall accuracy of f 15 per cent. The phototube was then used to measure the quantum yields of the in vitro firefly bioluminescence reaction and later, the quantum yield of the luminol chemiluminescence reaction in aqueous solution.(2)

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Section: Sources Of Errormentioning

confidence: 99%

Absolute Spectral Sensitivity of Phototubes and the Application to the Measurement of the Absolute Quantum Yields of Chemiluminescence and Bioluminescence*

Lee

Seliger

1965

Photochem & Photobiology

148

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“…There also appears to be a sharp drop at 450 nm of about 5%. A second iterative calculation [5] of eq. 3 using these variations in the relative values of R for the integration outside the principal transmission band yielded essentially the same results.…”

Section: Determination Of the Relative Spectral Responsivity Of The Thermopilementioning

confidence: 99%

“…In order to obtain a detector's spectral response one may measure its output relative either to a detector of known spectral response [1-4] * or to a set of lamp and filter combinations of known spectral irradiance [5][6][7][8]. The latter procedure is limited in resolution and accuracy by the size of the obtainable bandwidths .…”

Section: Introductionmentioning

confidence: 99%

Detector spectral response from 350 to 1200 nm using a monochromator based spectral comparator

Corróns¹,

Zalewski²

1978

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The. method o {, n.zlatlvz Apzctn.al detector KzAponAz mzaAun.zment baAzd on ^llteHA o ^known tn.anAmlttance and a ApzctKal ln.n.adlancz Atandan.d lamp uiaA uAzd to mea.Au.fie the HZAponAlvlty o £ a thznmopllz.The thzn.mopllz waA then uAzd In conj unction with a monochn.oma.toK baAzd Apec.tn.al companaton. to mzaAun.z the n.zlatlvz Apec.tn.al n.zAponAz ^nom 350 to 1200 nm o& Azvznal othzn. detecton.A .Seven.a.1 auxiliary zxpznlmzntA to evaluate the accunacy o{> theAe tzchnlquzA an.e dzAcn.lbzd.The zAtlmatzd ac.cun.acif o& nzlatlvz ApzctKal KZAponAz mzaAun.zmzntA uAlng theAe technlqueA and thlA pan.tlculan. lnAtn.umzntatlon waA ^ound to nange fanom 3 to 71 depending upon the type oh dztzcton. being me.aAun.zd and thz Apzctn.al n.zglon unden Atudy.Vlnally, the elective tn.anAmlttancz o{ Azvzn.al filltzH-A waA mzaAun.zd to zvaluatz thz accuracy o^thz n.zlatl\)Z Apzctn.al dztzcton nzAponAz mzaAunzmzntA .It waA concludzd that thz z^zctlvz tn.anAmlttancz tzAt Ia not a Kzllablz way to judgz thz accuracy o £ dztzcton H-ZAponAZ mzaAuH.zmzntA . KzyiMon.dA:Vztzcton.; dztzcton nadlomztn.y; dztzcton ApzctKal companaton; dztzcton. ApzctKal KZAponAz; illtzn tKanAmlttancz tzAt; photo dztzcton.; photomztzn.; nadlomztzn.; ApzctKal KZAponAlvltyv

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Optical spectra excited in high pressure noble gases by alpha impact

Bennett

1962

Annals of Physics

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Determination of the Relative Spectral Sensitivity of Phototubes*

Cited by 10 publications

References 2 publications

Absolute Spectral Sensitivity of Phototubes and the Application to the Measurement of the Absolute Quantum Yields of Chemiluminescence and Bioluminescence*

Absolute Spectral Sensitivity of Phototubes and the Application to the Measurement of the Absolute Quantum Yields of Chemiluminescence and Bioluminescence*

Detector spectral response from 350 to 1200 nm using a monochromator based spectral comparator

Optical spectra excited in high pressure noble gases by alpha impact

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