Determination of the penetration of 9-fluoropropyl-(+)-dihydrotetrabenazine across the blood–brain barrier in rats by microdialysis combined with liquid chromatography–tandem mass spectrometry

Zhou, Xue; Qiao, Jie; Yin, Wei; Zhu, Lin; Kung, Hank F.

doi:10.1016/j.jchromb.2011.08.043

Cited by 7 publications

(9 citation statements)

References 17 publications

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“…The LC-MS/MS method was optimized and validated as described previously (18,19). Chromatography analysis was performed on a Waters ACQUITY module (Waters, Milford, MA, USA).…”

Section: Lc-ms/ms Methods and Validationmentioning

confidence: 99%

“…When employed, appropriatelabeled compounds with short-lived radionuclides such as 11 C, 15 O, 13 N, and 18 F, PET can quantitate physiological processes in a non-invas iv e manner ( 7 ). (8,9).…”

Section: Introductionmentioning

confidence: 99%

“…In previous papers (18,19), we evaluated the effect of a pseudo-carrier on pharmacokinetics of AV-133 using a UPLC-MS/MS method, and the concentrations of AV-133 and its pharmacokinetics in the brain and blood of rats were also obtained by using microdialysis and LC-MS/MS. These results confirmed that LC-MS/MS is useful in the evaluation of radiopharmaceuticals.…”

Section: Introductionmentioning

confidence: 99%

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Mapping the Target Localization and Biodistribution of Non-Radiolabeled VMAT2 Ligands in rat Brain

Deng

Zhou

et al. 2014

AAPS J

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Abstract. Imaging targeting vesicular monoamine transporter (VMAT2) alterations is a sensitive tool for early diagnosis of Parkinson's disease. Our group has reported several novel 2-amino-DTBZ derivatives as potential VMAT2 imaging agents. The objective of this paper is to develop a non-radiolabeled methodology to screen the candidate compounds for accelerating the drug discovery process. 9- Furthermore, several novel 2-amino-DTBZ derivatives were detected using this methodology, and their biodistribution data in rat brain were obtained. The information about target engagements of candidates was provided.

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“…The LC-MS/MS method was optimized and validated as described previously (18,19). Chromatography analysis was performed on a Waters ACQUITY module (Waters, Milford, MA, USA).…”

Section: Lc-ms/ms Methods and Validationmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Mapping the Target Localization and Biodistribution of Non-Radiolabeled VMAT2 Ligands in rat Brain

Deng

Zhou

et al. 2014

AAPS J

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“…After equilibration for 1 h, the perfusate was collected and measured as the C dial . The in vivo recovery (R in vivo ) was calcualted by the ratio of (C perf C dial ) and C perf (Zhou et al, 2011). The cefuroxime lysine concentrations in brain microdialysis (C m ) were converted to the actual unbound concentrations in rat brain (C u ) as follows: C u = C m /R in vivo (Tai, 2003;Sun et al, 2009).…”

Section: Instrumental and Chromatographic Conditionsmentioning

confidence: 99%

Determination of cefuroxime lysine in rat brain microdialysates by ultra‐fast liquid chromatography with UV and tandem mass spectrometry: application to an acute toxicokinetic study

Zhao

Zhu

et al. 2014

Biomedical Chromatography

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Cefuroxime lysine is a new second-generation cephalosporins, which can penetrate the blood-brain barrier to cure the meningitis. In order to investigate its acute toxicokinetic study after intraperitoneal injection of 675 mg/kg cefuroxime lysine, a sensitive and clean ultra-fast liquid chromatography-tandem mass spectrometry (UFLC-MS/MS) method for the determination of cefuroxime lysine in microdialysate samples was developed and validated, which was compared with UFLC-UV as a reference method. Chromatographic separation was performed on a Shim-pack XR-ODS C18 column (75 × 3.0 mm, 2.2 µm), with an isocratic elution of 0.1% formic acid in acetonitrile-0.1% formic acid in water (45:55, v/v) for LC-MS and acetonitrile-20 mm potassium dihydrogen phosphate (pH 3.0,20:80, v/v) for LC-UV. The lower limit of detection was 0.01 µg/mL for LC-MS and 0.1 µg/mL for LC-UV method, with the same corresponding linearity range of 0.1-50 µg/mL. The intra- and inter-day precisions (relative standard deviation) for both methods were from 1.1 to 8.9%, while the accuracy was all within ±10.9%. The results of both methods were finally compared using paired t-test; the results indicated that the concentrations measured by the two methods correlated significantly (p < 0.05), which suggested that the two methods based on LC-MS and LC-UV were suitable for the acute toxicokinetic study.

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“…6 In order to achieve better temporal resolution and higher relative in vivo recovery, sample fractions are typically collected in 10-30 min intervals at low ow rates of 1.0-3.0 mL min À1 . [7][8][9] For the property of small volume and low analytes concentration in the dialysate, a highly sensitive and selective detecting technique is necessary. Liquid chromatography coupled with tandem mass spectrometry (LC-MS/MS) is a powerful tool for determining trace compounds in complex biological matrices.…”

Section: Introductionmentioning

confidence: 99%

Application of online microdialysis coupled with liquid chromatography-tandem mass spectrometry method in assessing neuroprotective effect of Rhizoma coptidis on diabetic rats

Wang

Zhang

et al. 2015

Anal. Methods

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Determination of the penetration of 9-fluoropropyl-(+)-dihydrotetrabenazine across the blood–brain barrier in rats by microdialysis combined with liquid chromatography–tandem mass spectrometry

Cited by 7 publications

References 17 publications

Mapping the Target Localization and Biodistribution of Non-Radiolabeled VMAT2 Ligands in rat Brain

Mapping the Target Localization and Biodistribution of Non-Radiolabeled VMAT2 Ligands in rat Brain

Determination of cefuroxime lysine in rat brain microdialysates by ultra‐fast liquid chromatography with UV and tandem mass spectrometry: application to an acute toxicokinetic study

Application of online microdialysis coupled with liquid chromatography-tandem mass spectrometry method in assessing neuroprotective effect of Rhizoma coptidis on diabetic rats

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