Perkebunan Nusantara IV (Persero) is a state-owned enterprise that is engaged in the agro-industry business field. The purpose of this research is to analyze the mental workload of employees and determine the cause of the mental workload with the fishbone diagram method. The population of this study was 32 workers in the procurement and general affairs department, and the number of samples was 32 workers with a saturation sampling technique. The selected system was NASA-TLX, which is a method that calculates mental workload subjectively. This methodology measures the workload components: Mental Demand, Physical Demand, Temporal Demand, Own Performance, Effort, and Frustration. The results of the measurements show aspects of TD and MD most dominant by values of 37% and 31%. Based on the calculation of the final NASA TLX score, it is known that six employees have a high level of mental workload, 25 people have a medium mental workload, and 1 person has a very high mental workload. Based on the fishbone diagram, the factors that affect the cognitive burden on procurement and general employees are increased work activities and additional work outside the job description due to the reduced number of employees. In addition, the high time pressure at work caused by the short working time is the reason for the increased mental workload felt by employees.