Determination of the minimum suppressing concentration to metronidazole in representatives of obligate and optional anaerobic microflora of periodontal pockets

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BACKGROUND: Peri-implantitis is mainly caused by the activity of periodontal pathogens with insufficient oral hygiene, which causes the disintegration of intraosseous dental implants against the background of chronic inflammation in peri-implant tissues. The correct techniques in the post-prosthetic stage in patients with dental implants include medical examination with regular professional oral hygiene, which is complicated by low patient compliance. In this regard, there are high requirements for the level of individual oral hygiene and for the effectiveness of local hygiene products, among which dental rinses occupy an important place. As rinses, it is possible to use pine-containing substances, particularly a complex of oil-essential fir Solagift of a Siberian company (Tomsk). AIM: To conduct a microbiological study of the sensitivity of periodontopathogens and Candida albicans to the oil-essential complex of Siberian fir of different concentrations. MATERIAL AND METHODS: A number of periodontopathogens and C. albicans were cultured in the presence of a complex of oilessential Siberian fir in the following proportions: 1:5, 1:10, and 1:15. Incubation for up to 3 days was conducted in the Reverse Spinner RTS-1 bioreactor (BioSan, Latvia) with automatic analysis of the optical density of the culture (OD) at a wavelength of =850 nm. The optical density was measured in McFarland units (Mcf). The assessment of culture growth control was based on the analysis of the growth phases of periodontopathogens: adaptive (lag phase), exponential (log phase), stationary, and dying. The following clinical isolates of microorganisms were used: Streptococcus constellatus, Staphylococcus aureus, Fusobacterium nucleatum, Aggregatibacter actinomycetemcomitans, and C. albicans. RESULTS: Joint cultivation of periodontopathogens with the oilether complex of Siberian fir reduces the OD of cultures of clinical isolates when breeding coniferous substance 1:151:5 by 13.727.1% (A. actinomycetemcomitans), 18.362.0% (F. nucleatum), 30.056.4% (S. aureus), and 19.274.1% (S. constellatus). The studied coniferous complex suppresses the culture of C. albicans at a concentration of 1:5, reducing the OD of the culture of fungi in the microbiological experiment by 29.8%. CONCLUSION. Joint cultivation of periodontopathogens with the oilether complex of Siberian fir reduces the OD of cultures of clinical isolates when breeding coniferous substance 1:151:5. The studied coniferous complex suppresses the culture of C. albicans at a concentration of 1:5, reducing the OD of the culture of fungi in the microbiological experiment by 29.8%. The bacteriostatic effectiveness of the Siberian fir oil-ether complex significantly exceeds the effect of the herbal preparation Stomatophyte.

Section: материал и методыunclassified

Effect of the oil-ether complex of Siberian fir on the fungal and periodontopathogenic flora of the mouth (microbiological study)

Romanov¹,

Olesov²,

Царев³

et al. 2022

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“…В то же время местное применение антимикробных препаратов может оказывать кратковременный лечебный эффект, что связано с вымыванием препарата слюной и ротовой жидкостью. Кроме того, в связи с тем что метронидазол в пародонтологической практике применяется довольно давно и часто неконтролируемо, в последнее время появляется все больше сообщений о повышении резистентности патогенных микроорганизмов к данному препарату, что приводит к снижению эффективности применения метронидазола [6,7]. Для решения этой проблемы разрабатываются лекарственные формы, где метронидазол комбинируется с другими препаратами антибактериального действия.…”

Section: метронидазол в лечении заболеваний пародонтаunclassified

Combined use of antimicrobial medication and physical therapeutic factors in periodontitis

Tomaeva

Kodzaeva

Daurova

et al. 2022

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Metronidazole-based preparations are widespread in periodontal practice among those with antibacterial action. However, recently there are more and more reports about the increase in resistance of pathogenic microorganisms to this drug. The solution of this problem is facilitated by the combined effect of this drug and physical therapeutic factors, which can ensure the delivery of the antimicrobial agent to the periodontal tissues and its deposition. The efficacy of Metrogil Dent gel ultraphonophoresis with low-frequency ultrasound as well as Metrogil electrophoresis in the treatment of periodontitis has been proved. However, there is no information in the literature about the comparative effectiveness of ultraphonophoresis and electrophoresis of metronidazole-based preparations in the complex treatment of periodontitis, which is of scientific and practical interest.

Sensitivity of parodontopatogens to the cell juice of Siberian fir (microbiological investigation)

Romanov¹,

Ragulin

Olesov

et al. 2022

BACKGROUND: Search for new effective means of preventing inflammatory complications in the state of peri-implant tissues is relevant; in particular, products from coniferous greens produced by the Solagift company (Tomsk) are of interest. The bioeffective cell juice of the Siberian fir, the 100% carbon dioxide extract of Siberian fir (water fraction), stands out among them. Fir cell juice is recommended for enhancing immunity, preventing inflammatory diseases of the upper respiratory tract, and treating several pathological conditions. Fir juice contains vitamin C, carotene, polyphenolic compounds, flavonoids, iron, magnesium, copper, manganese, zinc, and maltoliron chelate complex (a natural antioxidant). AIM: This microbiological study aimed to examine the sensitivity of periodontal pathogens to bioeffective cell juice of Siberian fir of various concentrations. MATERIAL AND METHODS: A number of periodontal pathogens were cultivated in the presence of bioeffective Siberian fir cell juice in the following proportions: 1:5, 1:10, and 1:15. Incubation for up to 3 days was conducted in a reverse-spinner RTS-1 bioreactor (BioSan, Latvia) with automatic analysis of the optical density (OD) of the culture at a wavelength () of 850 nm. OD was measured in McFarland units (mcf). The assessment of culture growth control was based on the analysis of the growth phases of periodontal pathogens: adaptive (lag phase), exponential (log phase), stationary, and withering away. The following clinical isolates of microorganisms were used: Streptococcus constellatus, Staphylococcus aureus, Fusobacterium nucleatum, and Aggregatibacter actinomycetemcomitans. RESULTS: The microbiological study of the sensitivity of periodontal pathogens (such as S. constellatus, S. aureus, F. nucleatum, and A. actinomycetemcomitans) to bioeffective cell juice of Siberian fir showed a growth slowdown of microorganisms in the presence of fir juice and inhibition of the growth of a culture of periodontal pathogens when co-cultivated with fir juice. A significant decrease in the OD of cultures of all studied periodontal pathogens was observed at a fir juice concentration of 1:10 and 1: 5. At these concentrations, there was no growth of S. aureus culture, and the degree of decrease in the average OD of other periodontal pathogens reached 27.1% (A. actinomycetemcomitans), 31.8% (F. nucleatum), and 33.6% (S. constellatus).