Determination of the Minimum Population Size of Pumas (<i>Puma concolor</i>) Through Fecal DNA Analysis in Two Protected Cerrado Areas in the Brazilian Southeast

Miotto, Renata Alonso; Rodrigues, F. R. N.; Ciocheti, Giordano; Galetti, Pedro Manoel

doi:10.1111/j.1744-7429.2007.00315.x

Cited by 53 publications

(66 citation statements)

References 41 publications

(110 reference statements)

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“…We collected all fresh fecal samples of felids encountered in the field regardless of the sample size, as there is considerable overlap in feces morphology and dimensions between jaguars and other cat species present in the RNV, particularly the puma (Puma concolor Linnaeus, 1771) and ocelot (Leopardus pardalis Linnaeus, 1758) (FARRELL et al, 2000;HAAG et al, 2009;MIOTTO et al, 2007). We identified the cat species by searching for guard hairs (hair ingested by specimens during self-cleaning and eliminated in the feces) and comparing them with a reference collection (futher details can be found in DEL DUQUE Jr., 2012).…”

Section: Sample Collection and Laboratory Proceduresmentioning

confidence: 99%

First record of intestinal parasites in a wild population of jaguar in the Brazilian Atlantic Forest

Srbek‐Araujo

Santos

Almeida

et al. 2014

Rev. Bras. Parasitol. Vet.

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Small and isolated wildlife populations may be more susceptible to disease, which makes illness an important issue to investigate regarding the conservation of large carnivores. Here, we present the results of the first investigation of intestinal parasites in one of the last remaining populations of jaguars in the Brazilian Atlantic Forest. We studied parasites from fecal samples using three different techniques for parasitological examination: floatation in saturated sodium chloride solution, sedimentation and formalin-ether centrifugation. Intestinal parasites were detected in 70% of the analyzed samples, and seven taxa (mean = 3.7 taxa/sample) were identified. All the groups of parasites that were identified have been recorded in previous jaguar studies. However, the records of Class Trematoda and nematodes Trichuridae are the first evidence of these groups of worms in free-ranging jaguars in Brazil. Although our results do not provide conclusive evidence on the health of this jaguar population, given its very small size (approximately 20 animals) we stress the need to properly understand the dynamics of disease in this wild population and to evaluate the risk of contracting new diseases from domestic species inhabiting the neighboring areas. These represent imperative actions for the successful conservation of this threatened population of jaguar.Keywords: Panthera onca, parasitology, wildlife, worms. ResumoPopulações pequenas e isoladas são mais suscetíveis a doenças, o que torna a investigação de enfermidades uma questão importante para a conservação de grandes carnívoros. O presente estudo apresenta os resultados da primeira investigação de parasitos intestinais em uma das últimas populações remanescentes de onça-pintada na Mata Atlântica brasileira. Os parasitos foram obtidos a partir de amostras fecais, sendo utilizadas três técnicas para exame parasitológico: flutuação em solução saturada de cloreto de sódio (Método de Willis), sedimentação (Método de HPJ) e centrifugação com formol-éter. Parasitos intestinais foram detectados em 70% das amostras analisadas, tendo sido identificados sete táxons (média = 3,7 taxa/amostra). Todos os grupos de parasitos identificados foram mencionados em estudos anteriores realizados com onças-pintadas. Entretanto, os registros de Trematoda e nematoides Trichuridae aqui obtidos representam a primeira evidência desses grupos de vermes em onças-pintadas de vida livre no Brasil. Embora os resultados obtidos não sejam conclusivos quanto à saúde da população estudada, em virtude do tamanho populacional reduzido (aproximadamente 20 indivíduos), recomenda-se a investigação da dinâmica de doenças, bem como a avaliação do risco de contração de novas doenças a partir do contato com animais domésticos provenientes do entorno da área estudada. Essas investigações são fundamentais para a conservação dessa população ameaçada de onças-pintadas.Palavras-chave: Panthera onca, parasitologia, vida silvestre, vermes.

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Section: Sample Collection and Laboratory Proceduresmentioning

confidence: 99%

First record of intestinal parasites in a wild population of jaguar in the Brazilian Atlantic Forest

Srbek‐Araujo

Santos

Almeida

et al. 2014

Rev. Bras. Parasitol. Vet.

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“…A study in the northeastern São Paulo State -Brazil, in two protected areas, used samples of faeces and analysis of a portion of the mitochondrial gene (cytochrome b) to determine the presence of pumas and estimate their minimal population (Miotto et al, 2007). The results indicated that the mtDNA was able to differentiate the faeces of pumas from other felines present in the region, such as ocelot (Leopardus pardalis), amplifying in 60% of the samples collected.…”

Section: Molecular Markers In Felinesmentioning

confidence: 98%

Molecular Markers and Genetic Diversity in Neotropical Felids

Barufatti¹,

Moreno-Cotulio²

2012

Analysis of Genetic Variation in Animals

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“…Among Brazilian studies, we found only one published paper that used this method, Miotto et al (2007). The cited study only managed to genotype 9 of 32 feline samples collected in the field.…”

Section: Introductionmentioning

confidence: 99%

Amplifiability of mitochondrial, microsatellite and amelogenin DNA loci from fecal samples of red brocket deer Mazama americana (Cetartiodactyla, Cervidae)

Oliveira

Duarte²

2013

Genet. Mol. Res.

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ABSTRACT. We tried to amplify mitochondrial, microsatellite and amelogenin loci in DNA from fecal samples of a wild Mazama americana population. Fifty-two deer fecal samples were collected from a 600-ha seasonal semideciduous forest fragment in a subtropical region of Brazil (21°20ꞌS, 47°17ꞌW), with the help of a detection dog; then, stored in ethanol and georeferenced. Among these samples 16 were classified as "fresh" and 36 as "non-fresh". DNA was extracted using the QIAamp ® DNA Stool Mini Kit. Mitochondrial loci were amplified in 49 of the 52 samples. Five microsatellite loci were amplified by PCR; success in amplification varied according to locus size and sample age. Successful amplifications were achieved in 10/16 of the fresh and in 13/36 of the non-fresh samples; a negative correlation (R = -0.82) was found between successful amplification and locus size. Amplification of the amelogenin locus was successful in 22 of the 52 samples. The difficulty of amplifying nuclear loci in DNA samples extracted Amplifiability of deer fecal DNA from feces collected in the field was evident. Some methodological improvements, including collecting fresh samples, selecting primers for shorter loci and quantifying the extracted DNA by real-time PCR, are suggested to increase amplification success in future studies.

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Determination of the Minimum Population Size of Pumas (Puma concolor) Through Fecal DNA Analysis in Two Protected Cerrado Areas in the Brazilian Southeast

Cited by 53 publications

References 41 publications

First record of intestinal parasites in a wild population of jaguar in the Brazilian Atlantic Forest

First record of intestinal parasites in a wild population of jaguar in the Brazilian Atlantic Forest

Molecular Markers and Genetic Diversity in Neotropical Felids

Amplifiability of mitochondrial, microsatellite and amelogenin DNA loci from fecal samples of red brocket deer Mazama americana (Cetartiodactyla, Cervidae)

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