The Orbit Response Matrix method is applied to experimentally determine the Cooler ring optics at the Indiana University Cyclotron Facility (IUCF). We have carried out two experiments in Oct. and Nov. 1996 to measure the orbit response of the IUCF Cooler Ring. An analysis software was adopted from the NSLS, BNL[1]. In our analysis, strength error in quadrupoles and steering dipoles, and amplifier gain in BPMs are included as fitting parameters. However, effects of the linear and non-linear coupling are excluded in our preliminary analysis. Since the resolution of our BPM system is of the order of 10 µm, we will address the effect of BPM resolution on the applicability of the orbit response matrix method in proton storage rings.