Determination of the insecticide diflubenzuron in mushrooms by kinetic method and high-performance liquid chromatographic method

Abstract

In the present study, a new sensitive and simple kinetic-spectrophotometric method for the determination of the insecticide diflubenzuron [1-(4-chlorophenyl)-3-(2,6-diflubenzoil)urea] is proposed. The method is based on the inhibited effect of diflubenzuron on the oxidation of sulphanilic acid (SA) by hydrogen peroxide in phosphate buffer in presence Cu(II) ion. Diflubenzuron was determined with linear calibration graph in the interval from 0.31 to 3.1 μg mL⁻¹ and from 3.1 to 31.0 μg mL⁻¹. The optimized conditions yielded a theoretical detection limit of 0.18 μg mL⁻¹ corresponding to 0.036 mg kg(-1)mushroom sample based on the 3S(b) criterion. The RSD is 5.03-1.83 % and 2.81-0.71 % for the concentration interval of diflubenzuron 0.31-3.1 μg mL⁻¹ and 3.1-31.0 μg mL⁻¹, respectively. The reaction was followed spectrophotometrically at 370 nm. The kinetic parameters of the reaction are reported, and the rate equations are suggested. The developed procedure was successfully applied to the rapid determination of diflubenzuron in spiked mushroom samples of different mushroom species. The HPLC method was used like a comparative method to verify results.

Development and application of kinetic-spectrophotometric method for analysis of diflubenzuron in soil samples using SPE followed by HPLC method

“…In the previous work our laboratory developed and validated two kinetic methods for DFB determination. The first work reported DFB determination in the range 0.31 -3.10 μg/cm 3 and the application in mushroom samples (Grahovac et al, 2010). The second kinetic method reported DFB determination in the interval from 0.102 to 3.40 μg/cm 3 .…”

Development of a kinetic spectrophotometric method for insecticide diflubenzuron determination in water and baby food samples

“…So far, there has been only one report on determination of DFB by a kinetic method. Grahovac et al developed and validated a kinetic method for DFB determination in the range 0.31 -3.1 μg mL -1 [31]. This method was applied for determination of DFB in mushrooms with good recovery.…”