Determination of the insect growth regulator methoprene in wheat grain and milling fractions using an enzyme immunoassay

Hill, A. V.; Mei, Joanne V.; Yin, Chih Ming; Ferguson, Bruce S.; Skerritt, John H.

doi:10.1021/jf00010a040

Cited by 26 publications

(9 citation statements)

References 12 publications

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“…After 4 washes with PBS-0.05 % Tween, 200 µL/well of HRP-goat-anti-rabbit immunoglobulins (Dako, Carpinteria, CA) at 3.25 µg/L in 1 % BSA-PBS-Tween were added and incubated 30 min. After 4 washes, 150 µL of H 2 O 2 -3,3',5,5'-tetramethylbenzidine (Hill et al 1991) were incubated 30 mm, and after addition of 50 µL/well 1.25 M H 2 SO 4 , to stop the reaction the absorbance was read at 450 nm. Solidphase antibody competitive assays were also performed on plates coated with 100 µL/well of 10 ug/mL antibody in 0.05 M sodium carbonate buffer, pH 9.6.…”

Section: Methodsmentioning

confidence: 99%

Laboratory and Field Enzyme-Immunoassays for Diazinon and Their Application to Residue Analysis in Lanolin, Water, and Fruit Juice

Beasely

McAdam

Larkin³

et al. 1997

Bulletin of Environmental Contamination and Toxicology

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Section: Methodsmentioning

confidence: 99%

Laboratory and Field Enzyme-Immunoassays for Diazinon and Their Application to Residue Analysis in Lanolin, Water, and Fruit Juice

Beasely

McAdam

Larkin³

et al. 1997

Bulletin of Environmental Contamination and Toxicology

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“…Enzyme Immunoassay (EIA) Procedure. Since detailed instructions accompany the assay materials (available from Millipore Corp., Bedford, MA) and microtiter strip preparation has been described in recent work with wheat (Hill et al, 1991), only a brief synopsis will be given here. Tobacco (1.0 g, ground) is extracted for 30 min with agitation using 24 mL of 90% (v/v) aqueous acetonitrile.…”

Section: Methodsmentioning

confidence: 99%

Validation of an enzyme immunoassay for analysis of methoprene residues on tobacco

Heckman¹,

Ferguson²,

Fan³

et al. 1992

J. Agric. Food Chem.

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“…The conjugate concentrations used were the lowest concentrations that provided an optical density (OD) in the assay of 0.7-1.2 and are listed in Table 1. After washing the microwells five times with distilled water, color was developed by the addition of 150 µL/well 3,3′,5′-tetramethylbenzidine (Sigma)/peroxidebased substrate (Hill et al, 1991) for 30 min. Color development was stopped by addition of 50 µL/well 1.25 M sulfuric acid, and OD values were read at 450 nm using a microplate reader, interfaced with a personal computer and data fitted to a four-parameter logistic plot using SOFTmax software (Molecular Devices, Menlo Park, CA).…”

Section: Utilization Of Bis(p-chlorophenyl)ethanolmentioning

confidence: 99%

Development of a Panel of Immunoassays for Monitoring DDT, Its Metabolites, and Analogues in Food and Environmental Matrices

Beasley

Phongkham

Daunt

et al. 1998

J. Agric. Food Chem.

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A panel of antisera was prepared using analogues and derivatives of metabolites of the organochlorine insecticide, p,p′-DDT as haptens. The assays developed exhibited differing cross-reactions for different DDT analogues and metabolites, and the choice of hapten for the detecting enzyme conjugate had almost as much effect on assay specificity and sensitivity as the structure of the hapten used for antibody production. Those assays developed using hapten I, based on esters of bis(pchlorophenyl)acetic acid (DDA), typically detected DDA with greater sensitivity than p,p′-DDT or p,p′-DDE. The most sensitive assay for p,p′-DDT (lower limit of detection of 0.3 µg/L) was obtained using an immunogen based on bis(p-chlorophenyl)ethanol (hapten IV), although a significant crossreaction with dichlorodiphenyltrichloroethane (DDD) and DDE was obtained. The most specific assay for p,p′-DDT was obtained using an immunogen (hapten VI) that includes all elements of the DDT structure, except that one of the p-chloro groups was replaced by β-alanine carboxamide for coupling to carrier proteins. Antibodies based on a similar DDE hapten (V) exhibited specificity for p,p′-DDE over p,p′-DDT. Greater specificity and sensitivity for dicofol were obtained by using an immunogen derived from ester hydrolysis of chlorbenzilate (hapten II). The assays provided methods for detection of p,p′-DDT plus p,p′-DDE either by using the antibody raised to hapten IV with conjugate based on hapten Ib or by using the assay based on hapten V, with treatment of samples with warm alcoholic KOH, which converted DDT to DDE. Some of the immunoassays were applied to the detection of DDT and DDE in water, soil, and selected foods.

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Determination of the insect growth regulator methoprene in wheat grain and milling fractions using an enzyme immunoassay

Cited by 26 publications

References 12 publications

Laboratory and Field Enzyme-Immunoassays for Diazinon and Their Application to Residue Analysis in Lanolin, Water, and Fruit Juice

Laboratory and Field Enzyme-Immunoassays for Diazinon and Their Application to Residue Analysis in Lanolin, Water, and Fruit Juice

Validation of an enzyme immunoassay for analysis of methoprene residues on tobacco

Development of a Panel of Immunoassays for Monitoring DDT, Its Metabolites, and Analogues in Food and Environmental Matrices

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