The distinctive aroma and taste of roasted coffee can b e due to varietal differences in green coffee b eans. The purpose of this research was to map the chemical characteristics of green coffee b eans of Gayo arab ica and Gayo rob usta using principal component analysis (PCA) approach. Other analyses include pH using pH meter, total fats b y Soxhlet method, total ash b y gravimetric method, total protein b y Kjeldahl method, total carb ohydrates b y difference, total solub le solids (TSS) b y refractometer, organic acids composition b y HPLC-UV/Vis, and total amino acids composition b y UHPLC-PDAD. Six green coffee b ean samples of Gayo arab ica and Gayo rob usta from different locations in Gayo, Aceh, as well as samples of arab ica and rob usta coffee b eans each from plantation in West Java, Central Java and East Java (as comparators) were used in this study. The results showed the chemical characteristics of Gayo arab ica and Gayo rob usta could b e mapped b y PCA b ased on the concentrations of fats, organic acids and carb ohydrates. The organic acid composition of Gayo arab ica was associated with malic acid, succinic acid and citric acid, while Gayo rob usta to acetic acid. The organic acids as well as TSS of the coffee b eans could map Gayo arab ica separately from Java arab ica. Gayo arab ica had higher organic acids, whereas Java arab ica had higher TSS. Gayo rob usta were characterized b y higher fats, ash and total amino acids, b ut lower TSS than Java rob usta. This study suggested the distinct characteristics of Gayo coffee b eans to their corresponding coffee b eans from Java.