Determination of the geographical origin of Trachinotus ovatus and Pampus argenteus in China by multi-element and stable isotope analysis

Ni, Xinyu; Li, Xuyan; Ran, Guojing; Chen, Jiali; Jiang, Xinwei; Sun, Jianxia; Bai, Weibin

doi:10.1016/j.foodchem.2022.133457

Cited by 10 publications

(7 citation statements)

References 25 publications

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“…Similar variations in minerals were observed in food collected from various locations. 7,11 The identified markers by the VIP score are partially in agreement with the results of the heat map (Table 1 and Figure 2). Overall, our findings demonstrated that the adapted strategies were effective in identifying the cultivation sites of the BMSs and thus could be further applied for discrimination of other aromatic and medicinal seeds.…”

Section: Comparison Of the Bms Samples Based On Multi-element Composi...supporting

confidence: 85%

“…6,7 Liquid chromatographic coupled with mass spectrometry (LC-MS) and inductively coupled plasma mass spectrometry (ICP-MS) are among the powerful analytical methodologies applied for food authentication. [7][8][9][10][11][12][13][14] ICP-MS and LC-MS can screen for an area of cultivation of agricultural products by determining numerous elements and metabolites, allowing identification of chemical markers. Chemometric techniques of qualitative and quantitative data acquired from various analytical tools have also been employed for tracing the geographical origin of agricultural produces.…”

Section: Introductionmentioning

confidence: 99%

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Metabolic and elemental profiling as potential discriminating features among the black mahlab seeds (Monechma ciliatum) grown in three different regions

Mariod,

Tahir

2024

Phytochemical Analysis

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IntroductionBlack mahlab (Monechma ciliatum) seed is a rich source of metabolites and minerals and is usually believed to have a similar composition between different areas of cultivation. Until now, no studies have assessed changes in black mahlab seeds (BMSs) to determine those constituents that help to discriminate them according to geographical origin.ObjectivesThe present study attempted to compare the metabolomics and elemental profiles of BMSs of different geographical origins and identified the potential markers using ultrahigh‐performance liquid chromatography quadrupole Orbitrap tandem mass spectrometry (UHPLC‐Q‐Orbitrap‐MS2), and inductively coupled plasma mass spectrometry (ICP‐MS) techniques and established the chemometric model to identify the potential markers and discriminate them according to cultivation sites.Material and methodsIn this work, data from metabolites analysis by UHPLC‐Q‐Orbitrap‐MS2 and multi‐elemental data obtained from ICP‐MS were combined with chemometrics for tracing the geographical origin of BMSs. Principal component analysis (PCA) was used to evaluate the overall grouping of samples. In contrast, partial least squares‐discriminant analysis (PLS‐DA) and orthogonal partial least squares discriminant analysis (OPLS‐DA) were employed for authentication.ResultsPLS‐DA and OPLS‐DA models were fully validated (R2Y and Q2 values > 0.5). Variable importance of various projections was applied to obtain valuable data about differential elements (seven markers were identified) and metabolites (23 markers were identified) with high discrimination potential. The outcomes presented in this study serve as an appropriate framework for developing novel discrimination approaches in food origin screening.

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Section: Comparison Of the Bms Samples Based On Multi-element Composi...supporting

confidence: 85%

Section: Introductionmentioning

confidence: 99%

Metabolic and elemental profiling as potential discriminating features among the black mahlab seeds (Monechma ciliatum) grown in three different regions

Mariod,

Tahir

2024

Phytochemical Analysis

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“…Vibrio harveyi is the main threat to the large-scale farming of Trachinotus ovatus , an important commercial fish in China ( 30 , 31 ). In the present study, TroIGFBP5b was cloned and identified, and its different expression patterns were examined.…”

Section: Introductionmentioning

confidence: 99%

Insulin-like growth factor binding protein 5b of Trachinotus ovatus and its heparin-binding motif play a critical role in host antibacterial immune responses via NF-κB pathway

Zhou²,

Du³

et al. 2023

Front. Immunol.

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IntroductionInsulin-like growth factor binding protein 5 (IGFBP5) exerts an essential biological role in many processes, including apoptosis, cellular differentiation, growth, and immune responses. However, compared to mammalians, our knowledge of IGFBP5 in teleosts remains limited.MethodsIn this study, TroIGFBP5b, an IGFBP5 homologue from golden pompano (Trachinotus ovatus) was identified. Quantitative real-time PCR (qRT-PCR) was used to check its mRNA expression level in healthy condition and after stimulation. In vivo overexpression and RNAi knockdown method were performed to evaluate the antibacterial profile. We constructed a mutant in which HBM was deleted to better understand the mechanism of its role in antibacterial immunity. Subcellular localization and nuclear translocation were verified by immunoblotting. Further, proliferation of head kidney lymphocytes (HKLs) and phagocytic activity of head kidney macrophages (HKMs) were detected through CCK-8 assay and flow cytometry. Immunofluorescence microscopy assay (IFA) and dual luciferase reporter (DLR) assay were used to evaluate the activity in nuclear factor-κB (NF-κβ) pathway.ResultsThe TroIGFBP5b mRNA expression level was upregulated after bacterial stimulation. In vivo, TroIGFBP5b overexpression significantly improved the antibacterial immunity of fish. In contrast, TroIGFBP5b knockdown significantly decreased this ability. Subcellular localization results showed that TroIGFBP5b and TroIGFBP5b-δHBM were both present in the cytoplasm of GPS cells. After stimulation, TroIGFBP5b-δHBM lost the ability to transfer from the cytoplasm to the nucleus. In addition, rTroIGFBP5b promoted the proliferation of HKLs and phagocytosis of HKMs, whereas rTroIGFBP5b-δHBM, suppressed these facilitation effects. Moreover, the in vivo antibacterial ability of TroIGFBP5b was suppressed and the effects of promoting expression of proinflammatory cytokines in immune tissues were nearly lost after HBM deletion. Furthermore, TroIGFBP5b induced NF-κβ promoter activity and promoted nuclear translocation of p65, while these effects were inhibited when the HBM was deleted.DiscussionTaken together, our results suggest that TroIGFBP5b plays an important role in golden pompano antibacterial immunity and activation of the NF-κβ signalling pathway, providing the first evidence that the HBM of TroIGFBP5b plays a critical role in these processes in teleosts.

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“…Research endeavors have been conducted to ascertain the provenance and traceability of agricultural products using stable isotopes and elements combined with chemometrics: Cassiae Semen tea [21], Chinese garlic [18], Trachinotus ovatus and Pampus argenteus [22], avocado [9], durian [23], rice [24], apple juice concentrates [25], wine [26,27], and Mediterranean mussels [28]. Recently, Tanaka et al [29] used neodymium isotope ratio ( 143 Nd/ 144 Nd) to track the origin of Ruditapes philippinarum (clam) shells, and the results showed shell ƐNd is a powerful tool to discriminate the geographic location of clams.…”

Section: Introductionmentioning

confidence: 99%

Towards Verifying the Imported Soybeans of China Using Stable Isotope and Elemental Analysis Coupled with Chemometrics

Zhou,

Xiong,

et al. 2023

Foods

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Verifying the geographical origin of soybeans (Glycine max [Linn.] Merr.) is a major challenge as there is little available information regarding non-parametric statistical origin approaches for Chinese domestic and imported soybeans. Commercially procured soybean samples from China (n = 33) and soybeans imported from Brazil (n = 90), the United States of America (n = 6), and Argentina (n = 27) were collected to characterize different producing origins using stable isotopes (δ2H, δ18O, δ15N, δ13C, and δ34S), non-metallic element content (% N, % C, and % S), and 23 mineral elements. Chemometric techniques such as principal component analysis (PCA), linear discriminant analysis (LDA), and BP–artificial neural network (BP-ANN) were applied to classify each origin profile. The feasibility of stable isotopes and elemental analysis combined with chemometrics as a discrimination tool to determine the geographical origin of soybeans was evaluated, and origin traceability models were developed. A PCA model indicated that origin discriminant separation was possible between the four soybean origins. Soybean mineral element content was found to be more indicative of origin than stable isotopes or non-metallic element contents. A comparison of two chemometric discriminant models, LDA and BP-ANN, showed both achieved an overall accuracy of 100% for testing and training sets when using a combined isotope and elemental approach. Our findings elucidate the importance of a combined approach in developing a reliable origin labeling method for domestic and imported soybeans in China.

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Determination of the geographical origin of Trachinotus ovatus and Pampus argenteus in China by multi-element and stable isotope analysis

Cited by 10 publications

References 25 publications

Metabolic and elemental profiling as potential discriminating features among the black mahlab seeds (Monechma ciliatum) grown in three different regions

Metabolic and elemental profiling as potential discriminating features among the black mahlab seeds (Monechma ciliatum) grown in three different regions

Insulin-like growth factor binding protein 5b of Trachinotus ovatus and its heparin-binding motif play a critical role in host antibacterial immune responses via NF-κB pathway

Towards Verifying the Imported Soybeans of China Using Stable Isotope and Elemental Analysis Coupled with Chemometrics

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