Determination of the effect of calcineurin inhibitors on the rat's immune system after KLH immunisation

Development and Validation of a Canine T-Cell-Dependent Antibody Response Model for Immunotoxicity Evaluation

“…The assessment of TDAR in canines exposed to immunosuppressive compounds has not been previously described, however studies in rats (Gore et al, 2004;Roman et al, 2004) evaluated the effects of immunosuppressive compounds on the KLH specific antibody responses. Differences in the magnitude of IgG and IgM specific responses were observed, with the magnitude of the IgG suppression being considerably greater that the IgM.…”

“…The in vivo primary T-cell dependent antibody response (TDAR) has been recommended to assess the acquired immune response in species used in preclinical toxicology studies. The measurement of primary responses (IgM and IgG) to T-cell dependent antigens such as keyhole limpet hemocyanin (KLH) has been described in mice (Moynihan et al, 2000), rats (Moraska and Fleshner, 2001;Smith et al, 2003;Gore et al, 2004;Roman et al, 2004), dogs (Mates and Hinton, 1976;Weissman et al, 1992;Jones et al, 1993), rabbits (Mates and Hinton, 1976), pigs (Bruininx et al, 2000), non-human primates (Martin et al, 2004), and humans (Korver et al, 1984;Snyder et al, 1990). Various routes of administration have been described: intraperitoneal (mice and rats), intramuscular (pigs and non-human primates), intrapulmonary (dogs and rabbits), and subcutaneous (rats and humans).…”

“…Reports of evaluation of TDAR in the presence of potential immunomodulators have been described for rats (Koller and Exon 1983;Conner et al, 2001;Smith et al, 2003;Gore et al, 2004;Roman et al, 2004) and humans (Amlot et al, 1986); however, studies evaluating the effect of immunosuppressive compounds on the TDAR to KLH in canines have not been reported. Hence, the objectives of these studies were to evaluate different doses of KLH, routes of immunization and kinetics of the IgM and IgG response to KLH.…”

Head-down tilt posture elicits transient lymphocyte mobilization from the iliac, but not mesenteric, lymph nodes of rats

“…The data were measured and analyzed using Lysys II software and BD Cell Quest, respectively (Becton Dickinson); 10 4 cells were examined for each sample. In addition, we analyzed the cellular components of collected lymph from the iliac lymph nodes in the control and HDT rats after immunization with a mixture of a keyhole limpet hemocyanin (KLH, Thermo Scientific) and an adjuvant (TiterMax Gold, TiterMax) (9,21,28,35). The KLH (100 g/part) and adjuvant (100 l/part) were subcutaneously injected into both sides of the groin in 5-wk-old male Wistar rats.…”

An in vivo role of bone morphogenetic protein-6 in aldosterone production by rat adrenal gland

“…The animals received normal food containing 0.24% sodium (MF, Oriental Yeast, Tokyo, Japan), with water provided ad libitum. Three-week-old male SD rats were actively immunized with rat BMP-6 (mature domain: QSQDVSRGSSASDYN, 15 amino acids) antigen conjugated with keyhole limpet hemocyanin (KLH) at the C-terminus (SigmaGenosys Japan, Ishikari) as reported previously [12][13][14]. SD rats were initially immunized with 0.1 mg/animal of either KLH antigen alone (control-KLH, n = 6) or mature BMP-6-conjugated KLH (BMP-6-KLH, n = 6) on day 0 and then boosted with 0.1 mg/animal of each antigen at weeks 2, 4 and 6.…”