Determination of the dietary lysine requirement by measuring plasma free lysine concentrations in rainbow trout Oncorhynchus mykiss after dorsal aorta cannulation

Yun, Hyeonho; Park, Gunjun; Ok, Imho; Katya, Kumar; Hung, Silas S.O.; Bai, Sungchul C.

doi:10.1186/s41240-016-0004-1

Cited by 16 publications

(14 citation statements)

References 38 publications

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“…This value is higher than the values reported for other commonly cultured fishes (Wilson, ). Examples include rainbow trout Oncorhynchus mykiss, 60.1 and 61.0 g/kg dietary protein (Ketola, ; Yun et al., ), stinging catfish Heteropneustes fossilis , 61 g/kg dietary protein (Khan, ), catla Catla catla, 62 g/kg dietary protein (Ravi & Devaraj, ), Japanese sea bass, Lateolabrax japonicus 58‐61 g/kg dietary protein (Mai et al., ), grouper Ephinephelus coioides 55.6 g/kg dietary protein (Luo et al., ), Indian major carp Cirrhinus mrigala 57.5 g/kg dietary protein (Ahmed & Khan, ), beluga Huso huso 55 g/kg dietary protein (Hoseini, Hosseini, & Soudagar, ). The variation in the estimated lysine requirements reported for different fish species is probably due to a number of factors including dietary protein source, age, strains, essential amino acids profile and dietary energy content (De Silva, Gunasekera, & Gooley, ; Forster & Ogata, ); fish size, levels of other nutrients and experimental conditions (Kim, Kayes, & Amundson, ); response criteria and the mathematical model used to estimate the requirement value (Mai et al., ; Zhou et al., ).…”

Section: Discussionmentioning

confidence: 99%

Dietary lysine requirement of juvenile dusky kob, Argyrosomus japonicus

2017

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To determine dietary lysine requirement of dusky kob, Argyrosomus japonicus, six isonitrogenous and isoenergetic diets (431 g/kg crude protein, 141 g/kg lipid and 20 kJ/kg) were formulated with graded levels of crystalline L‐lysine (18–42 g/kg of the dry diet). The protein source in the basal diet comprised fishmeal and soya, where a combination of L‐aspartic and L‐glutamic acids was maintained at a ratio of 1:1, and all diets were supplemented with a mixture of crystalline essential amino acids to simulate the amino acid profile in dusky kob. Dietary treatments were randomly assigned to triplicate groups of 12 fish (4.5 ± 0.2 g, mean weight; 66.5 ± 1.1 mm, total length ± SD), which were fed to apparent satiation three times daily for 12 weeks. The fish fed dietary L‐lysine at 21, 29 and 33 g/kg dry diet showed the highest specific growth rates (SGR) and the lowest feed conversion ratio. For most amino acids, retention in the body of the fish increased with an increase in dietary lysine from 18 to 21 g/kg, and it reached a maximum somewhere between 21 and 33 g/kg, where after amino acid retention decreased with increasing dietary lysine. Based on SGR and using segmented broken‐line analysis, the dietary L‐lysine requirement of juvenile dusky kob was estimated at 31.7 ± 1.6 g/kg dry diet corresponding to 73.5 g lysine per kg protein.

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Section: Discussionmentioning

confidence: 99%

Dietary lysine requirement of juvenile dusky kob, Argyrosomus japonicus

2017

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“…The fish were euthanized by 100 mg l −1 high dose of MS 222 (Yun et al . ) (Sigma ® , Germany) before dissection. The intestinal tracts were removed from the abdominal cavity, and the content was squeezed out and harvested separately (Wu et al .…”

Section: Methodsmentioning

confidence: 99%

Effects of different diets on the intestinal microbiota and immunity of common carp ( Cyprinus carpio )

Yang

Luo

et al. 2019

J Appl Microbiol

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Aims: An 8-week trial was performed to evaluate the differences in the intestinal microbiota and immune function of common carp (Cyprinus carpio)fed different diets. Methods and Results: The fish (initial weight 492Á6 AE 30 g, n = 270) were randomly divided into three groups with three replicates and were fed earthworms (group A), earthworms + duckweed (group M) or duckweed (group P) respectively. The diversity and dominant microbiota of the intestinal bacteria were detected by denaturing gradient gel electrophoresis, and the abundance of dominant bacteria was quantified by qPCR. Additionally, the activities of some nonspecific immune enzymes and antioxidant enzymes were determined. The results showed that higher diversity and abundance of intestinal microbiota were observed in group M and group P (P < 0Á05). Based on the intestinal microbiota, Cetobacterium was only detected in the intestines of common carp in group A, and Bacillus was identified in groups M and P. Additionally, a higher abundance of Bacteroidetes and Firmicutes was also found in the intestine in group P than in group A (P < 0Á05). Interestingly, the higher activities of immune enzymes were detected in intestine of common carp in group M, such as acid phosphatase, phosphatase (AKP), lysozyme, total antioxidant capacity, superoxide dismutase, catalase, glutathione peroxidase. In addition, the lower level of metabolites were also detected, such as nitric oxide and malondialdehyde. Conclusions: Our results indicate that the intestinal microbiota and intestinal immunity of common carp were affected by diet. Meanwhile, the results show that a mixed diet can promote and improve the immune function of the omnivorous carp intestine, which suggests that paddy fields might be more suitable for the intestinal health and animal welfare of omnivorous fishes because they contain plant and animal diets. Significance and Impact of the Study: As an ecological aquaculture strategy, the rice-fish mode has attracted attention among farmers, researchers and even consumers, especially for the cultivation of common carp in paddy fields. In paddy fields, fish can eat plant-and animal-based diets. However, it is not clear whether common carp feeding on a mixed diet in paddy fields have better intestinal health. This experiment is one of only a few studies performed from the perspective of intestinal micro-organisms and immunity to successfully study the effects of different natural diets on adult common carp. This study also provides a theoretical basis for healthy breeding of common carp in paddy fields.

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“…The circulating level of essential and non-essential amino acids in fish is mainly influenced by the quality of the administered feed (YUN & al [24]; YUN & al [25]) and the voluntary intake rate. Fish are poikilothermic organisms and any changes in medial parameters lead to behavioral and physiological changes (SARAVANAN & al [26]; HOLT & JØRGENSEN [27]).…”

Section: Resultsmentioning

confidence: 99%

Amino acid levels from blood plasma of rainbow trout raised in different seasons and farming systems

Cocan¹,

Constantinescu²,

Răducu³

et al. 2019

Rom Biotechnol Lett.

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Climate change and large variations in medial parameters over the last decades, question the future of salmonid farming. For this, recirculating aquatic systems (RAS) have been developed, that artificially provide medial parameters at optimal values. To optimize these aquatic systems, fish are reared at high densities (50-100 kg / m 3 of water). From this point of view, due to high growth densities, the quality and biological value of trout meat can be depreciated. The results of our study show a relatively constant level of essential amino acids (EAA) and non-essential (NEAA) regardless of season for rainbow trout raised in RAS compared to those raised in the classic system. With small exceptions, both for EAA and NEAA, their values regardless of the season were higher in the case of RAS compared to the classic growth system. High level of valine (Val E Autumn 168.40 ± 1.14 μg / ml) is highlighted for the EAA compared with the level obtained in winter in the classical system (Val C Winter 105.40 ± 1.72 μg / ml). In NEAA, very high values were obtained for alanine (Ala E Autumn 117.60 ± 0.56 μg / ml) as compared to the same amino acid values in the winter season in the classical growth system (Ala C Winter 74.53 ± 0.67 μg / ml). The seasonal variations of EAA and NEAA in classical trout are due to environmental conditions, especially temperature, which influence the voluntary ingestion of feed, trout being poikilotherm organisms. Optimal media parameters in recirculating systems contribute to a higher biological value of trout, highlighted by the increased and constant level of EAA and NEAA.

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Determination of the dietary lysine requirement by measuring plasma free lysine concentrations in rainbow trout Oncorhynchus mykiss after dorsal aorta cannulation

Cited by 16 publications

References 38 publications

Dietary lysine requirement of juvenile dusky kob, Argyrosomus japonicus

Dietary lysine requirement of juvenile dusky kob, Argyrosomus japonicus

Effects of different diets on the intestinal microbiota and immunity of common carp ( Cyprinus carpio )

Amino acid levels from blood plasma of rainbow trout raised in different seasons and farming systems

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