Determination of the concentrations of oligosaccharides, complex type carbohydrates, and glycoproteins using the phenol-sulfuric acid method

Saha, Sanjoy Kumar; Brewer, C. Fred

doi:10.1016/0008-6215(94)84249-3

Cited by 292 publications

(110 citation statements)

References 15 publications

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“…Biantennary complex glycopeptides were isolated from human fibrinogen and human IgG as described earlier (Stanley, 1987). The concentrations of carbohydrates were measured by the phenolsulfuric acid method using appropriate mixture of Man, Glc, and Gal as the standard (Dubois et al, 1956;Saha and Brewer, 1994). Purity of the oligosaccharides was checked by 500-MHz 'H-NMR spectroscopy.…”

Section: Methodsmentioning

confidence: 99%

A Comparison of the Fine Saccharide‐Binding Specificity of Dioclea grandiflora Lectin and Concanavalin A

Gupta

Oscarson

Raju

et al. 1996

European Journal of Biochemistry

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The lectin from the seeds of Dioclea grandifora (DGL) is a Man/Glc-specific tetrameric protein with physical and saccharide-binding properties reported to be similar to that of the jack bean lectin concanavalin A (ConA). Unlike other plant lectins, both DGL and ConA bind with high affinity to the core trimannoside moiety, 3,6-di-O-(a-~-mannopyranosyl)-a-~-mannopyranoside, which is present in all asparagine-linked carbohydrates. In the present study, hemagglutination inhibition techniques have been used to investigate binding of DGL and ConA to a series of mono-and dideoxy analogs of methyl 3,6-di-0-(a-D-mannopyranosy1)-a-D-mannopyranoside and to a series of asparagine-linked oligomannose and complex oligosaccharides and glycopeptides. The results indicate that both DGL and ConA recognize epitopes on all three residues of the trimannoside: the 3-, 4-, and 6-hydroxyl groups of the a(1-6)Man residue, the 3-hydroxyl group of the a(1-3)Man residue, and the 2-and 4-hydroxyl groups of the central Man residue of the core trimannoside. However, unlike ConA, DGL does not bind to biantennary complex carbohydrates. This was confirmed by showing that biantennary complex glycopeptides do not bind to a DGL-Sepharose affinity column. Unlike ConA, DGL does not show enhanced affinity for a large Nlinked oligomannose carbohydrate (Man9 glycopeptide) relative to the trimannoside. Thus, DGL and ConA share similar epitope recognition of the core trimannoside moiety. However, they exhibit differences in their fine specificities for larger N-linked oligomannose and complex carbohydrates.Keywords: lectin; specificity ; cross-linking ; carbohydrate ; glycopeptide.The seed lectin from Dioclea grandifora (DGL) is a M a d Glc-binding protein isolated from the plant found in North East Brazil. The lectin is devoid of covalently linked carbohydrate, and is reported to be a tetramer with a molecular mass of 100 kDa (Moreira et al., 1983). Like other legume lectins, DGL requires Ca2+ and Mn2+ for its binding activity. The lectin possesses a high degree of sequence similarity with the jack bean lectin concanavalin A (ConA), another member of the Dioclea tribe, and differs in only 52 out of 237 residues (Richardson et al., 1984). Six of the seven residues that have been implicated as ligands for Ca2+ and Mn2+ are conserved, and the seven amino acid residues surrounding the shallow saccharide-binding NMRD, nuclear magnetic relaxation dispersion, the magnetic field dependence of nuclear magnetic relaxation rate (longitudinal) of mlvent protons; CHO, Chinese hamster ovary. 1984).

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Section: Methodsmentioning

confidence: 99%

A Comparison of the Fine Saccharide‐Binding Specificity of Dioclea grandiflora Lectin and Concanavalin A

Gupta

Oscarson

Raju

et al. 1996

European Journal of Biochemistry

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“…16 Total carbohydrate content in the fractions was determined by the phenol -sulfuric acid method. 17 …”

Section: Fractionation Of Acetone Seed Extractmentioning

confidence: 99%

In vitroandin silicointeraction of porcine α-amylase withVicia fabacrude seed extract and evaluation of antidiabetic activity

Choudhary

Mishra

2016

Bioengineered

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Diabetes mellitus is a group of metabolic disorder of glucose metabolism. The proper management of blood glucose level is an indicator in the treatment of this complex pathology. The aim of the present study was to evaluate the inhibitory potential of Vicia faba crude seed extracts on the activities of a-amylase. Phytochemical screening, FTIR and HPLC analysis confirmed the presence of a phenolic compound with percentage yield of 9.87% in the acetone extract. Acetone extract of seed had highest inhibitory potential against porcine a-amylase (IC 50 value of 2.94 mg/mL). Kinetic analysis revealed that the acetone and methanol extract displayed mixed mode of inhibition toward a-amylase. In-silico analysis was agreement with in-vitro studies in which phenolic compounds (catechin, epicatechin, gallic acid, and proanthocyanidin) showed more negative free energy against standard drug (acarbose) and bound with catalytic residues of a-amylase. These results might be due to the synergistic action of constituents present in seed extract or acting separately.

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“…Volatile suspended solids (VSS) represented as the biomass concentration was measured according to Standard Methods (APHA 1995). The sugar concentration in fermentative medium was determined according to the phenol-sulfuric acid method using sucrose as a standard (Saha & Brewer 1994).…”

Section: Methodsmentioning

confidence: 99%

Biohydrogen Productions from Hydrolysate of Water Hyacinth Stem (Eichhornia crassipes) Using Anaerobic Mixed Cultures

Pattra¹,

Sittijunda²

2017

JSM

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Response surface methodology (RSM) with central composite design (CCD) was applied to optimize key factors affecting hydrogen production (HP Kaedah permukaan tindak balas (RSM) dan pusat reka bentuk tergubah (CCD) digunakan untuk mengoptimumkan faktor utama yang mempengaruhi pengeluaran hidrogen (HP) daripada hidrolisat asid cair batang keladi bunting (WHS) melalui enapcemar anaerobik terawat-haba di dalam proses penapaian kumpulan. Faktor utama yang mempengaruhi kepekatan substrat dan pH awal dikaji. Keputusan menunjukkan bahawa kepekatan substrat dan pH awal telah memberi kesan

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Determination of the concentrations of oligosaccharides, complex type carbohydrates, and glycoproteins using the phenol-sulfuric acid method

Cited by 292 publications

References 15 publications

A Comparison of the Fine Saccharide‐Binding Specificity of Dioclea grandiflora Lectin and Concanavalin A

A Comparison of the Fine Saccharide‐Binding Specificity of Dioclea grandiflora Lectin and Concanavalin A

In vitroandin silicointeraction of porcine α-amylase withVicia fabacrude seed extract and evaluation of antidiabetic activity

Biohydrogen Productions from Hydrolysate of Water Hyacinth Stem (Eichhornia crassipes) Using Anaerobic Mixed Cultures

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