Determination of the Concentration of Diallyl Trisulfide in Rat Whole Blood Using Gas Chromatography with Electron-Capture Detection and Identification of Its Major Metabolite with Gas Chromatography Mass Spectrometry

Abstract

A simple, rapid, and sensitive procedure has been developed using gas chromatography with electron-capture detection to measure diallyl trisulˆde levels in rat blood. Blood samples were acidiˆed, and the analyte was extracted with hexane, and then degradation was stopped with acetonitrile before gas chromatographic separation. Two calibration curves were linear over the range of 10-500 ng/ml and 0.2-20 mg/ml, with typical r values of 0.9986 and 0.9993, respectively. The structure of its major metabolite was conˆrmed using combined gas chromatography-mass spectrometry. The limit of detection was less than 10 ng/ml, and the assay was highly reproducible, giving peaks with excellent chromatographic properties. The method is suitable for pharmacokinetic and therapeutic purposes.

Mitochondria-mediated apoptosis by diallyl trisulfide in human prostate cancer cells is associated with generation of reactive oxygen species and regulated by Bax/Bak

“…15, 16, 18, 19 and the present study) is evident at concentrations (10 -40 Amol/L) that are well within the pharmacologically achievable range based on a recent pharmacokinetic study (43). The peak plasma concentration of DATS in rats following treatment with 10 mg DATS was shown to be about 31 Amol/L (43).…”

Forkhead Box Q1 Is a Novel Target of Breast Cancer Stem Cell Inhibition by Diallyl Trisulfide

“…For example, ALDH1 activity is inhibited by about 50% in the presence of 5 M DATS (this study), whereas a much higher concentration is necessary to induce apoptosis in proliferating mammary tumor cells (19). Nevertheless, the DATS concentration required to inhibit bCSC is likely achievable because the maximum blood concentration was shown to be ϳ31 M in rats after a single intravenous injection of 10 mg of DATS (45).…”

Activation of a novel ataxia-telangiectasia mutated and Rad3 related/checkpoint kinase 1–dependent prometaphase checkpoint in cancer cells by diallyl trisulfide, a promising cancer chemopreventive constituent of processed garlic

“…The DATS-mediated cell growth inhibition and cell cycle arrest is observed at concentrations (10,20, and 40 Amol/L) within the range pharmacologically achievable based on a recent pharmacokinetic study (59). The peak plasma concentration of DATS in rats following treatment with 10 mg DATS has been shown to be about 31 Amol/L (59).…”