Determination of the compressive modulus of elasticity of periodontal ligament derived from human first premolars

Limjeerajarus, Nuttapol; Sratong-on, Pimpet; Dhammayannarangsi, Phetcharat; Tompkins, Kevin; Kamolratanakul, Paksinee; Phannarus, Krisadi; Osathanon, Thanaphum; Limjeerajarus, Chalida Nakalekha

doi:10.1016/j.heliyon.2023.e14276

Cited by 3 publications

(2 citation statements)

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“…36,37 As for PDL, fibroblasts could modify ECM compositions and structures, thereby altering the width of PDL in response to mechanical force. 22,38 In the present study, comparison of PDL and DP at the single-cell level effectively ruled out the heterogeneity of cells resulting from their distinct origins and identified a population of fibroblasts unique to PDL. Importantly, mechanoresponsive ECM genes were highly expressed in this identified PDL-specific fibroblast subtype, implicating these cells as the mechanoresponsive effectors within the dental microenvironment.…”

Section: Discussionmentioning

confidence: 72%

“…The association between fibroblasts and mechanical responses has been investigated in a variety of fiber connective tissue, such as heart and bladder tissue 36,37 . As for PDL, fibroblasts could modify ECM compositions and structures, thereby altering the width of PDL in response to mechanical force 22,38 . In the present study, comparison of PDL and DP at the single‐cell level effectively ruled out the heterogeneity of cells resulting from their distinct origins and identified a population of fibroblasts unique to PDL.…”

Section: Discussionmentioning

confidence: 74%

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ScRNA‐seq links dental fibroblasts heterogeneity with mechanoresponsiveness

Dai

Wang

et al. 2023

J of Periodontal Research

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Background and objective: Periodontal ligament (PDL) and dental pulp (DP) share a common origin but have distinct biological and mechanical functions. To what extent the mechanoresponsive property of PDL can be attributed to its unique transcriptional profiles of cellular heterogeneity is unclear. This study aims to decipher cellular heterogeneity and distinct mechanoresponsive characteristics of odontogenic soft tissues and their underlying molecular mechanisms. Materials and Methods: A single-cell comparison of digested human periodontal ligament (PDL) and dental pulp (DP) was performed using scRNA-seq. An in vitro loading model was constructed to measure mechanoresponsive ability. Dual-luciferase assay, overexpression, and shRNA knockdown were used to investigate the molecular mechanism. Results: Our results demonstrate striking fibroblast heterogeneity across and within human PDL and DP. We demonstrated that a tissue-specific subset of fibroblasts existed in PDL exhibiting high expression of mechanoresponsive extracellular matrix (ECM) genes, which was verified by an in vitro loading model. ScRNA-seq analysis indicated a particularly enriched regulator in PDL-specific fibroblast subtype, Jun Dimerization Protein 2 (JDP2). Overexpression and knockdown of JDP2 extensively | 801 XU et al.

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