Determination of the Antioxidant Capacity of Herbal Extracts Using a Biosensor Based on Laccase Trametes Versicolor

Abstract

The polyphenolic compounds are antioxidant molecules having potentially beneficial implications to human health, so presently there is a strong interest in finding natural sources of antioxidants. Therefore, this work presents the results of the spectrophotometric studies using the DPPH radical method for the determination of the antioxidant capacity equivalent to caffeic acid in different extracts of Kohleria deppeana (topoya), Sedum morganianum (cola de borrego) and Alternanthera repens (tianguispepetla).The spectrophotometric method is contrasted with an electrochemical method using a biosensor based on the enzyme Laccase Trametes Versicolor immobilized on a polymeric membrane of polyvinyl alcohol.