Determination of the antileukemic drug mitoguazone and seven other closely related bis(amidinohydrazones) in human blood serum by high-performance liquid chromatography

Koskinen, Mikko; Elo, Hannu; Lukkari, P.; Riekkola, Marja Liisa

doi:10.1016/0378-4347(96)00126-0

Cited by 3 publications

(3 citation statements)

References 37 publications

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“…Guanylhydrazones are widely studied molecules of great scientific interest due to their broad biological potential, which includes antihypertensive, antidiabetic, antimalarial, trypanocidal, anti-bacterial and antitumoral activities [ 1 , 2 , 3 , 4 , 5 , 6 , 7 , 8 , 9 , 10 ]. Andreani et al synthesized and evaluated the biological activity of some new guanylhydrazones against a series of lung, breast and glioma tumors.…”

Section: Introductionmentioning

confidence: 99%

“…UHPLC is based on the use of pumps that can operate at higher pressures and columns with smaller particles, with diameters lower than 2.2 µm, allowing higher efficiency and resolution and shorter run times [ 19 ]. There are some analytical methods for the quantification of commercially available synthetic guanylhydrazones by HPLC [ 9 , 20 , 21 ], although there are few methods for the quantification of the recently synthesized ones, but there are no analytical methods for the analysis of the guanylhydrazones reported in this work.…”

Section: Introductionmentioning

confidence: 99%

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Development and Validation of HPLC-DAD and UHPLC-DAD Methods for the Simultaneous Determination of Guanylhydrazone Derivatives Employing a Factorial Design

et al. 2017

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Guanylhydrazones are molecules with great pharmacological potential in various therapeutic areas, including antitumoral activity. Factorial design is an excellent tool in the optimization of a chromatographic method, because it is possible quickly change factors such as temperature, mobile phase composition, mobile phase pH, column length, among others to establish the optimal conditions of analysis. The aim of the present work was to develop and validate a HPLC and UHPLC methods for the simultaneous determination of guanylhydrazones with anticancer activity employing experimental design. Precise, exact, linear and robust HPLC and UHPLC methods were developed and validated for the simultaneous quantification of the guanylhydrazones LQM10, LQM14, and LQM17. The UHPLC method was more economic, with a four times less solvent consumption, and 20 times less injection volume, what allowed better column performance. Comparing the empirical approach employed in the HPLC method development to the DoE approach employed in the UHPLC method development, we can conclude that the factorial design made the method development faster, more practical and rational. This resulted in methods that can be employed in the analysis, evaluation and quality control of these new synthetic guanylhydrazones.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Development and Validation of HPLC-DAD and UHPLC-DAD Methods for the Simultaneous Determination of Guanylhydrazone Derivatives Employing a Factorial Design

et al. 2017

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“…Some authors had studied other guanylhydrazones by HPLC [33,34]. Other analytic non-thermal techniques have been used to identify and quantify samples with HPLC and FTIR [35,36].…”

Section: Introductionmentioning

confidence: 99%

Thermal characterization of some guanylhydrazones by thermal and nonthermal techniques

galvão

Brito

Dantas

et al. 2014

J Therm Anal Calorim

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2-[(3,5-di-tert-butyl-4-hydroxyphenyl)methylene]-hydrazinecarboximidamide (WE010), 2-([1,1 0 -biphenyl]-4-ylmethylene)-hydrazinecarboximidamide (WE014), and 2-[(3,4-dichlorophenyl)methylene]-hydrazinecarboximidamide (WE017) are guanylhydrazone derivatives widely studied biologically and chemically; however, there are no studies regarding their thermal behaviors. The present study aims to apply the thermal analyses: differential scanning calorimetry (DSC), differential scanning calorimetry coupled to the photovisual system (DSC-photovisual), and thermogravimetry (TG), to characterize the guanylhydrazones, as well as HPLC and FTIR. The DSC curve of WE010 shows a melting process with T onset at 190°C and peak at 193.5°C (DH 41.0 J g -1 ). Due to the symmetry of the melting peaks obtained by DSC, it is possible to calculate the purity of the sample (98.87 %). The DSC curve of WE014 shows the melting process in the range of 208-213°C, with a melting peak at 211°C (DH 61 J g -1 ).The DSC curve of WE017 showed T onset at 215°C and peak temperature of 219°C (DH 55 J g -1 ). The TG curve of WE010, WE014, and WE017 presents initial decomposition temperatures of 186.95, 197.31, and 195.44°C, respectively. The DSC-photovisual confirmed the results of DSC and TG. The HPLC determined the purities of the samples and confirmed the results of DSC. The FTIR confirmed the thermal data. Thus, the use of thermal analysis is an important tool for the characterization of molecules with therapeutic potential contributing to the characterization and evaluation of their stability as well as nonthermal technique with complementary tool.

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Rapid and sensitive quantitation of the antiproliferative agent mitoguazone in small volumes of plasma by high-performance liquid chromatography with ultraviolet detection

Cheng

Lin

Chou

2003

Journal of Chromatography B

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Determination of the antileukemic drug mitoguazone and seven other closely related bis(amidinohydrazones) in human blood serum by high-performance liquid chromatography

Cited by 3 publications

References 37 publications

Development and Validation of HPLC-DAD and UHPLC-DAD Methods for the Simultaneous Determination of Guanylhydrazone Derivatives Employing a Factorial Design

Development and Validation of HPLC-DAD and UHPLC-DAD Methods for the Simultaneous Determination of Guanylhydrazone Derivatives Employing a Factorial Design

Thermal characterization of some guanylhydrazones by thermal and nonthermal techniques

Rapid and sensitive quantitation of the antiproliferative agent mitoguazone in small volumes of plasma by high-performance liquid chromatography with ultraviolet detection

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