Determination of the Activity of Lipoxygenase (Lipoxidase)

Grossman, Shlomo; Zakut, Rina

doi:10.1002/9780470110454.ch5

Cited by 106 publications

(27 citation statements)

References 92 publications

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“…Antioxidant activity was determined by measuring the inhibition by a 1% polysaccharide solution of the autooxidation of linoleic acid at 37 • C for 24 h. A stock solution of 3 × 10 −2 M linoleic acid in Tween 20 was prepared as follows (Grossman & Zakut, 1979): 3 nmoles of linoleic acid were added to 50 mL distilled water containing 1 mL Tween 20. 3 to 5 mL 1 N NaOH were then added to clarify the resulting emulsion, and the volume was adjusted to 100 mL with distilled water.…”

Section: Antioxidant Activitymentioning

confidence: 99%

Antioxidant activity of the polysaccharide of the red microalga Porphyridium sp

et al. 2005

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The cells of the red microalga Porphyridium UTEX 637 are encapsulated within a sulfated polysaccharide whose external part (i.e., the soluble fraction) dissolves into the medium. It is thought that the main function of the polysaccharide is to protect the algal cells from the extreme environmental conditions, such as drought and high light, prevailing in their native sea-sand habitat. In this study, we evaluated the antioxidant properties of the watersoluble polysaccharide of Porphyridium sp. by determining the ability of a polysaccharide solution to inhibit: (1) autooxidation of linoleic acid, as determined by the standard thiobarbituric acid (TBA) and ferrous oxidation (FOX) assays; and (2) oxidative damage to 3T3 cells as determined by the dichlorofluorescein (DCFH) assay. In all three assays, the polysaccharide inhibited oxidative damage in a dose-dependent manner. Antioxidant activity was also exhibited by fractions of the polysaccharide obtained by sonication followed by separation on a reverse-phase HPLC with a C 8 semi-preparative column. It is suggested that the antioxidant activity of the sulfated polysaccharide protects the alga against reactive oxygen species produced under high solar irradiation, possibly by scavenging the free radicals produced in the cell under stress conditions and transporting them from the cell to the medium.

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Section: Antioxidant Activitymentioning

confidence: 99%

Antioxidant activity of the polysaccharide of the red microalga Porphyridium sp

et al. 2005

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“…Peroxyl radical was assayed in a system composed by linoleic acid/lipoxygenase/crocin following the rationale of Huang, Ou, and Prior (2005), with the modification of the peroxyl radical generation, here obtained from the reaction between linoleic acid as substrate and soybean lipoxygenase (Chamulitrat & Mason, 1989;Grossman & Zakut, 1979). This method was validated and compared with others in a previous work (Lo Scalzo, Todaro, & Rapisarda, 2012).…”

Section: Peroxyl Radicals Scavengingmentioning

confidence: 99%

Cooking influence on physico-chemical fruit characteristics of eggplant (Solanum melongena L.)

et al. 2016

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“…The aldehyde released was expressed as micrograms formaldehyde per gram of seed fresh weight. Lipoxygenase II (EC 1.13.11.13) enzyme activity was assayed as per the procedure described by Grossman and Zakut [7]. The results were expressed as U/min/g of seed fresh weight.…”

Section: Lipid Peroxidation Studiesmentioning

confidence: 99%

Biochemical Phenotyping of Soybean [Glycine max (L.) Merill] Genotypes to Establish the Role of Lipid Peroxidation and Antioxidant Enzymes in Seed Longevity

et al. 2013

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Thirty-three soybean [Glycine max (L.) Merill] genotypes, comprising 14 black-seeded and 19 yellow-seeded ones, were selected on the basis of their reported storability for the biochemical phenotyping to establish the role of lipid peroxidation and antioxidant enzymes in seed longevity. The present study revealed clear genotypic variability with respect to storability among different soybean genotypes. Good-storer genotypes with lower electrolyte leakage were characterized by smaller seed size with black testa color. The level of volatile aldehydes released and lipoxygenase II enzyme activity were higher in the yellow-seeded genotypes than in the black-seeded genotypes, though it increased in all during ageing. A sharp increase in the release of volatile aldehydes and lipoxygenase II activity, concomitant with the reduction in germination under uncontrolled laboratory conditions of storage indicated the role of lipid peroxidation in seed longevity behavior (r = -0.6638** and r = -0.7639**, respectively). No significant difference was noted in the mean hydroperoxide lyase activity of black and yellow-seeded genotypes. However, maintenance of high hydroperoxide lyase activity during storage resulted in higher release of volatile aldehydes and poor storability of seeds. Significantly higher antioxidant enzyme activity was recorded in the black-seeded genotypes than in the yellow-seeded ones, though there was a reduction in hydroperoxide lyase activity during storage in all the genotypes. The viability of black-seeded genotypes after storage for 1 year was better than the yellow-seeded genotypes.

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Determination of the Activity of Lipoxygenase (Lipoxidase)

Cited by 106 publications

References 92 publications

Antioxidant activity of the polysaccharide of the red microalga Porphyridium sp

Antioxidant activity of the polysaccharide of the red microalga Porphyridium sp

Cooking influence on physico-chemical fruit characteristics of eggplant (Solanum melongena L.)

Biochemical Phenotyping of Soybean [Glycine max (L.) Merill] Genotypes to Establish the Role of Lipid Peroxidation and Antioxidant Enzymes in Seed Longevity

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