Determination of the absolute quantum yield of fluorescent solutions

The photophysics of LOV-based fluorescent proteins — new tools for cell biology

“…It should also be mentioned that we were unable to find a publication that actually determined the fluorescence quantum yield of FMN. Van den Berg and coworkers 58 cite a Φ F of 0.26 for FMN with reference to 59 , were FMN is not mentioned whereas Holzer and colleagues 60 refer to a Φ F of 0.26 with reference to 56 , where the Φ F of riboflavin, but not FMN was determined. In this study we measured Φ F = 0.246 ± 0.002 for FMN, which is identical to the value we measured for riboflavin (Φ F = 0.247 ± 0.007).…”

“…However, the comparison of the fluorescence spectra of a fluorescent dye with known Φ F (standard) and that of the fluorophore under investigation is error prone, since it requires proper correction for differences in absorption and fluorescence spectra. The instrumental setup with the integrating sphere that has been used here was first verified by measuring the quantum yield (ΦF) of the well characterized fluorescent dye fluorescein in 0.1M NaOH(aq) (published ΦF between 0.91 and 0.95 55 ; measured ΦF = 0.931 ± 0.008), as well as riboflavin (published Φ F = 0.26 56 ; measured Φ F = 0.247 ± 0.007) and flavin adenine dinucleotide (published Φ F = 0.03 57 ; measured Φ F = 0.032 ± 0.0004). All three determined fluorescence quantum yields (low, intermediate and high) are in excellent agreement with the literature data and prove the high accuracy of measurements with an integrating sphere.…”

Membrane-Anchored DNA Assembly for Energy and Electron Transfer

“…19 Fluorescence melting: Melting curves were measured on a Varian Eclipse spectrofluorimeter equipped with a programmable multi-cell temperature block. The temperature was cycled between 10 and 55°C at 0.3°C/min.…”

Biology and the Exploration of Mars

“…1961]: the ratio of the intensities of the x-and y-polarized components of the emission as a function of the wavelength of the exciting light which is x-polarized; (4) the absolute quantum yield [Weber, 1957]: the fractional number of photons emitted per photon absorbed; (5) the excited state lifetime [Steingraber and Herlman, 1963]: the time interval in which the intensity of emission falls to 1/ e of its initial value.…”