Determination of Tetracyclines in Milk by Graphene-Based Solid-Phase Extraction and High-Performance Liquid Chromatography

A new composite nanofiber of polyacrylonitrile doped with copper benzene-1,3,5tricarboxylate metal-organic framework was fabricated by electrospinning and used as a microsorbent in the solid-phase extraction of trace tetracycline. The chemical structure of the adsorbent was studied by X-ray diffraction, scanning electron microscopy, Fourier transform infrared spectroscopy, Brunauer-Emmett-Teller surface area analysis, and Barrett-Joyner-Halenda pore size and volume analysis techniques. The significant parameters of the method including desorption solvent kind and volume, adsorbent mass, pH, and salt percentage were investigated. At the optimized conditions, the linear range was 8-1000 μg/L with a determination coefficient (R 2 ) of 0.9954. The limits of detection and quantification were 2.40 and 8.00 μg/L, respectively. The inter-and intraday precisions were 4.7 and 3.4%, respectively. The developed extraction method was followed by high-performance liquid chromatography and applied for the determination of tetracycline in human blood plasma, and good relative recoveries (97.3-104.5%) were obtained. K E Y W O R D Santibiotics, electrospun composite nanofibers, high-performance liquid chromatography, metal-organic frameworks, polyacrylonitrile 1500

Section: Resultsmentioning

confidence: 99%

Electrospun metal‐organic framework/polyacrylonitrile composite nanofibrous mat as a microsorbent for the extraction of tetracycline residue in human blood plasma

Arabsorkhi

Sereshti

Abbasi

2019

“…This method showed a linearity range of 2–1000 μg/mL, LOD and LOQ from 10 to 20 and 20 to 50 μg/kg, respectively. High recovery factors (between 82.3 and 103.6%) and a capacity to reuse the SPE cartridge for at least 50 times were qualities of this approach reporting the potential of graphene as a sorbent material .…”

Section: Off‐line Techniquesmentioning

confidence: 96%

The role of graphene‐based sorbents in modern sample preparation techniques

Toffoli

Maciel

Fumes

et al. 2017

The application of graphene-based sorbents in sample preparation techniques has increased significantly since 2011. These materials have good physicochemical properties to be used as sorbent and have shown excellent results in different sample preparation techniques. Graphene and its precursor graphene oxide have been considered to be good candidates to improve the extraction and concentration of different classes of target compounds (e.g., parabens, polycyclic aromatic hydrocarbon, pyrethroids, triazines, and so on) present in complex matrices. Its applications have been employed during the analysis of different matrices (e.g., environmental, biological and food). In this review, we highlight the most important characteristics of graphene-based material, their properties, synthesis routes, and the most important applications in both off-line and on-line sample preparation techniques. The discussion of the offline approaches includes methods derived from conventional solid-phase extraction focusing on the miniaturized magnetic and dispersive modes. The modes of microextraction techniques called stir bar sorptive extraction, solid phase microextraction, and microextraction by packed sorbent are discussed. The on-line approaches focus on the use of graphene-based material mainly in on-line solid phase extraction, its variation called in-tube solid-phase microextraction, and on-line microdialysis systems.

“…HPLC is the most commonly used method in the detection of antibiotic residues because of high separation and satisfactory quantitation performance [4–6]. Owing to the complexity of the food matrix composition and relatively low concentration of TCs residues, an effective matrix separation, purification, and preconcentration procedure are usually required prior to detection [7], such as SPE [8, 9], LLE [10, 11], and matrix solid‐phase dispersion (MSPD) [2, 12]. Nevertheless, large consumption of organic solvent, tedious procedures, and low enrichment factors are inevitably coupled with these methodologies.…”

Section: Introductionmentioning

confidence: 99%

Preparation of hydrophilic molecularly imprinted solid‐phase microextraction fiber for the selective removal and extraction of trace tetracyclines residues in animal derived foods

Lü

et al. 2020

The present work reported a novel hydrophilic and selective solid-phase microextraction fiber by improved multiple co-polymerization method immobilization of tetracycline molecularly imprinted polymer on a stainless steel wire and directly coupled with high-performance liquid chromatography for sensitive determination of trace tetracyclines residues in animal derived foods. The developed molecularly imprinted polymer coated solid-phase microextraction fibers were characterized through scanning electron microscopy, Fourier transfer infrared spectroscopy, thermogravimetric analysis, and adsorption experiments, the fiber with cross-linked and porous structure was observed and high thermal and chemical stability. The maximum adsorption capacity of this fiber with good selectivity reached 2.35 µg/mg in aqueous matrices, and showed good repeatability (relative standard deviation ≤ 6.6%, n = 5) and satisfying reproducibility between fiber to fiber (relative standard deviation ≤ 7.8%, n = 5). Under the optimized solid-phase microextraction conditions, satisfactory linearity (5-1000 µg/L) and detection limits (0.38-0.72 µg/kg, S/N = 3) for all the tetracyclines were obtained. The practicality of this method was proved by adding tetracycline, oxytetracycline at three levels to milk, chicken, and fish samples with good recoveries of 77.3-104.4%. K E Y W O R D Shigh-performance liquid chromatography, hydrophilic molecularly imprinted polymers, solid-phase microextraction, tetracyclines 2172