Determination of Tetracycline in Capsule Dosage Form

Abstract

Background: Tetracycline antibiotic is widely prescribed that encourages the finding of rapid and reliable protocol for the determination of tetracycline hydrochloride in hard gelatine capsules. Recently, the standard addition method is used commonly with the aid of a UV-visible (UV-vis) spectrophotometer for such purpose. In this work, the authors aim to quantify the percent assay of Apcycline-250 capsules utilizing the standard addition protocol. Methods: Twenty filled and emptied capsules were weighed and an equivalent amount of 50 mg of tetracycline hydrochloride was diluted, filtered, and an appropriate standard was spiked and prepared for assay. Results: The λmax was found to be 348 nm and the absorbance vs. concentration standard addition curve was plotted for tetracycline hydrochloride capsules. This curve shows good linearity (R2 = 0.9994). The limit of detection (LoD) and limit of quantification (LoQ) were observed as 0.007937 and 0.02646, respectively. While the percent assay was 103.2%. Conclusion: The authors conclude that the standard addition technique is a reliable method to conduct rapid, economical, and validated day-by-day analyses work of tetracycline hydrochloride hard gelatine capsules.