Determination of testosterone propionate in human plasma by gas chromatography— mass spectrometry

Baba, Shigeo; Fujioka, Minoru; Shinohara, Yoshihiko; Furuta, Takahisa

doi:10.1016/0378-4347(85)80033-5

Cited by 17 publications

(12 citation statements)

References 15 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The estimated average half‐life of one day is in accordance with previously reported values . Furthermore, the detection time is comparable to a pharmacokinetic study reported by Baba et al ., in which 25 mg of testosterone propionate‐d3 was administrated intramuscularly to one healthy volunteer. De la Torre et al .…”

Section: Resultssupporting

confidence: 90%

“…When testosterone ester preparations are applied, either orally or as a depot injection, the ester diffuses slowly into the blood stream. Although the cleavage process by esterase enzymes starts immediately, a detectable proportion of the pro‐drug, the testosterone ester itself, is still detectable in blood . It has been demonstrated that the esters testosterone propionate and testosterone enanthate can be successfully detected in human blood subsequent to intramuscular injections with detection times up to 4 and 11 days, respectively.…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Detection of testosterone esters in blood

Forsdahl

Erceg

Geisendorfer

et al. 2015

Drug Testing and Analysis

View full text Add to dashboard Cite

Injections of synthetic esters of testosterone are among the most common forms of testosterone application. In doping control, the detection of an intact ester of testosterone in blood gives unequivocal proof of the administration of exogenous testosterone. The aim of the current project was to investigate the detection window for injected testosterone esters as a mixed substance preparation and as a single substance preparation in serum and plasma. Furthermore, the suitability of different types of blood collection devices was evaluated. Collection tubes with stabilizing additives, as well as non-stabilized serum separation tubes, were tested. A clinical study with six participants was carried out, comprising a single intramuscular injection of either 1000 mg testosterone undecanoate (Nebido(®)) or a mixture of 30 mg testosterone propionate, 60 mg testosterone phenylpropionate, 60 mg testosterone isocaproate, and 100 mg testosterone decanoate (Sustanon(®)). Blood was collected throughout a testing period of 60 days. The applied analytical method for blood analysis included liquid-liquid extraction and preparation of oxime derivatives, prior to TLX-sample clean-up and liquid chromatography-tandem mass spectrometry (LC-MS/MS) detection. All investigated testosterone esters could be detected in post-administration blood samples. The detection time depended on the type of ester administered. Furthermore, results from the study show that measured blood concentrations of especially short-chained testosterone esters are influenced by the type of blood collection device applied. The testosterone ester detection window, however, was comparable.

show abstract

Section: Resultssupporting

confidence: 90%

Section: Introductionmentioning

confidence: 99%

Detection of testosterone esters in blood

Forsdahl

Erceg

Geisendorfer

et al. 2015

Drug Testing and Analysis

View full text Add to dashboard Cite

show abstract

“…Moreover, comparing the sensitivity of the developed method to previously described assays detecting testosterone esters in plasma, the LODs are comparable [24,26] or lower. Moreover, comparing the sensitivity of the developed method to previously described assays detecting testosterone esters in plasma, the LODs are comparable [24,26] or lower.…”

Section: Limit Of Detection (Lod)mentioning

confidence: 86%

“…Some reports can be found in the literature on the analysis of such esters in human and animal blood plasma using mass spectrometry (MS), [23][24][25][26][27][28] in addition to several methods for detecting steroid esters in hair. [28][29][30][31][32][33][34][35][36] Occasionally, derivatization prior to liquid chromatography-tandem mass spectrometry (LC-MS/MS) analysis was employed.…”

Section: Introductionmentioning

confidence: 99%

Screening of testosterone esters in human plasma

Forsdahl

Vatne

Geisendorfer

et al. 2013

Drug Testing and Analysis

View full text Add to dashboard Cite

The detection of an intact ester of testosterone in plasma is leading towards unequivocal proof of the administration of exogenous testosterone. In the current study, a sensitive screening method for the detection of nine testosterone esters in human plasma was developed. By preparing oxime derivatives of intact testosterone esters, the sensitivity of the assay was increased. Furthermore, the method included liquid-liquid extraction (LLE) as sample clean-up, as well as online separation of the target analytes from the derivatization solution. The analysis was performed by liquid chromatography (LC) coupled to tandem mass spectrometry (MS/MS). The method developed herein is simple and rapid, and was validated according to World Anti-Doping Agency (WADA) guidelines.

show abstract

“…One publication on the validation of a bioanalytical method for testosterone analysis concluded that TU is stable in blood [9]. Other methods developed to measure testosterone in plasma samples were published during the last decades [10,11]; however, even if these methods gave acceptable performance, they were not developed using enzymatic inhibitors and did not avoid the potential conversion of the TU and DHTU into testosterone and DHT.…”

mentioning

confidence: 99%

Importance of Measuring Testosterone in Enzyme-Inhibited Plasma for Oral Testosterone Undecanoate Androgen Replacement Therapy Clinical Trials

Lachance

Dhingra²,

Bernstein³

et al. 2015

Future Sci. OA

View full text Add to dashboard Cite

Aim:Testosterone undecanoate (TU) is metabolized by nonspecific esterases in blood to testosterone (T). Typical clinical practice has been to analyze testosterone in human serum. The degradation of TU to testosterone was evaluated in conditions typically used in clinical studies.Methods & Results:Freshly collected whole blood was fortified with TU at known concentration. Serum was prepared and T concentration was determined by LC–MS/MS. It was observed that TU degrades extensively to T in human blood under conditions typical of harvesting serum causing overestimation of T concentration of up to 243%. These results were confirmed in a clinical study in which serum and plasma samples were compared.Conclusion:It was demonstrated that T must be analyzed in enzyme-inhibited plasma when TU is the administered medication.

show abstract

Determination of testosterone propionate in human plasma by gas chromatography— mass spectrometry

Cited by 17 publications

References 15 publications

Detection of testosterone esters in blood

Detection of testosterone esters in blood

Screening of testosterone esters in human plasma

Importance of Measuring Testosterone in Enzyme-Inhibited Plasma for Oral Testosterone Undecanoate Androgen Replacement Therapy Clinical Trials

Contact Info

Product

Resources

About