Determination of testosterone and corticosterone in feathers using liquid chromatography-mass spectrometry

Bílková, Zuzana; Adámková, Marie Kotasová; Albrecht, Tomáš; Šimek, Zdeněk

doi:10.1016/j.chroma.2018.12.069

Cited by 8 publications

(7 citation statements)

References 28 publications

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“…A significant difference between the two methods is an additional derivatisation step in the latter that aims to address some reliability issues with cortisone measurements, whilst SPE could be automated in the former. As can be seen in Table 17: , the sensitivity of the method described by Bílková et al (2019) compares favourably with that of the Koren et al (2012a) method. Mi et al (2014) (reference 18009 in Annex B -) describes a method to measure simultaneously a total of 26 steroids, including testosterone and five other androgens in eggs from hen, quail, duck and pigeon.…”

Section: B321 Hypothalamic-pituitary-gonad Axis Hormones Androgensmentioning

confidence: 92%

“…This systematic evidence map uncovered two LC-MS/MS methods to measure androgens in feathers. Koren et al (2012a) (reference 2229 in Annex B -) determined testosterone and corticoids in the feathers of wild house sparrows, whereas Bílková et al (2019) (reference 7209 in Annex B -) recently optimised an analytical method that also simultaneously determines testosterone and cortisone from feather samples collected from the flanks of seven different species of passerine birds. A significant difference between the two methods is an additional derivatisation step in the latter that aims to address some reliability issues with cortisone measurements, whilst SPE could be automated in the former.…”

Section: B321 Hypothalamic-pituitary-gonad Axis Hormones Androgensmentioning

confidence: 99%

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Data collection in support of the Endocrine Disruption (ED) assessment for non‐target vertebrates

Martin

Ermler

McPhie

et al. 2020

EFS3

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To harmonise vertebrate OECD Test Guidelines for endocrine disruption testing between mammalian and non-mammalian test species, additional Estrogen, Androgen, Thyroid and Steroidogenesis (EATS) modality endpoints in non-mammalian models need to be assessed. These would mean for example the addition of hormonal measurements in fish, birds and amphibians. Furthermore, a better reporting of gross pathology findings for birds would also be considered advantageous for the assessment of endocrine disrupting properties. To facilitate adoption of additional measures, guidance on how to perform, report and evaluate these new endpoints is required. In this report, a variety of methods including a systematic evidence map, an extensive literature review and a survey of ecotoxicology laboratories were adopted to collect data on the topic. The systematic evidence map uncovered a range of methods for measuring sex and thyroid hormones in fish, birds and amphibians, although methods for measuring sex-hormones in fish, were by far, the most frequently encountered in the literature and laboratory survey. However, there are still considerable gaps in knowledge for: optimum sample timing for hormonal measurement (diurnal, developmental stage, etc.), issues with inherent variability, low sample volume (plasma/serum), test species selection, possible impacts of housing/diet/stress. The extensive literature review revealed that although gross pathology and histopathology have been used to investigate the effects of endocrine disrupting chemicals in birds, there are no standardised methods for assessment or interpretation, although relative weight of endocrine organs is frequently used as a gross pathology metric. Reports of how histopathology was assessed varied considerably. From the survey, few contract laboratories are experienced in conducting the avian test guideline and these types of pathology techniques. Recommendations for future work with non-mammalian taxa include: investigating the optimal time (or timings) for measuring hormones, developing non-invasive hormone measuring techniques, gaining knowledge of baseline/control hormonal data, and further developing guidance on conducting and assessing gross pathology and histopathology in birds.

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Section: B321 Hypothalamic-pituitary-gonad Axis Hormones Androgensmentioning

confidence: 92%

Section: B321 Hypothalamic-pituitary-gonad Axis Hormones Androgensmentioning

confidence: 99%

Data collection in support of the Endocrine Disruption (ED) assessment for non‐target vertebrates

Martin

Ermler

McPhie

et al. 2020

EFS3

View full text Add to dashboard Cite

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“…O-(3-trimethylammonium propyl) hydroxylamine bromide is a quaternary aminooxy (QAO) reagent, which has an aminooxy (-ONH 2 ) functional group as a carbonyl reactive group, and also processes a permanent positive charge, giving a better ionization efficiency in ESI-MS. As compared with HA, the signal-to-noise (S/N) ratio of QAO reagent for testosterone analysis is about 10 times higher than that of HA [93]. Furthermore, the use of QAO reagent can improve the reliability of hormone analysis in the complex matrix due to the formation of chromatographically separated derivative isomers [94].…”

Section: Hydroxylaminesmentioning

confidence: 99%

Progress and Challenges in Quantifying Carbonyl-Metabolomic Phenomes with LC-MS/MS

2021

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Carbonyl-containing metabolites widely exist in biological samples and have important physiological functions. Thus, accurate and sensitive quantitative analysis of carbonyl-containing metabolites is crucial to provide insight into metabolic pathways as well as disease mechanisms. Although reversed phase liquid chromatography electrospray ionization mass spectrometry (RPLC-ESI-MS) is widely used due to the powerful separation capability of RPLC and high specificity and sensitivity of MS, but it is often challenging to directly analyze carbonyl-containing metabolites using RPLC-ESI-MS due to the poor ionization efficiency of neutral carbonyl groups in ESI. Modification of carbonyl-containing metabolites by a chemical derivatization strategy can overcome the obstacle of sensitivity; however, it is insufficient to achieve accurate quantification due to instrument drift and matrix effects. The emergence of stable isotope-coded derivatization (ICD) provides a good solution to the problems encountered above. Thus, LC-MS methods that utilize ICD have been applied in metabolomics including quantitative targeted analysis and untargeted profiling analysis. In addition, ICD makes multiplex or multichannel submetabolome analysis possible, which not only reduces instrument running time but also avoids the variation of MS response. In this review, representative derivatization reagents and typical applications in absolute quantification and submetabolome profiling are discussed to highlight the superiority of the ICD strategy for detection of carbonyl-containing metabolites.

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“…Two MS/MS transitions of each pteridine were used for quantitative LC-MS/MS analyses. The conditions of triple quadrupole instrument optimised for analyses of pteridines were as follows: drying gas temperature 290 °C, drying gas flow 11 L/min, nebulizer gas pressure 45 psi, sheath gas temperature 350 °C, sheath gas flow 10 L/min, capillary voltage 3 500 V 70 . SRM transition of MS/MS analyses of pteridines are summarized in Table 6.…”

Section: Lc-ms/ms Analysis Liquid Chromatograph Agilent 1290 Infinitmentioning

confidence: 99%

Identification and age-dependence of pteridines in bed bugs (Cimex lectularius) and bat bugs (C. pipistrelli) using liquid chromatography-tandem mass spectrometry

Křemenová

Balvín

Otti

et al. 2020

Sci Rep

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Determining the age of free-living insects, particularly of blood-sucking species, is important for human health because such knowledge critically influences the estimates of biting frequency and vectoring ability. Genetic age determination is currently not available. Pteridines gradually accumulate in the eyes of insects and their concentrations is the prevailing method. Despite of their stability, published extractions differ considerably, including for standards, for mixtures of pteridines and even for light conditions. This methodological inconsistency among studies is likely to influence age estimates severely and to hamper their comparability. Therefore we reviewed methodological steps across 106 studies to identify methodological denominators and results across studies. Second, we experimentally test how different pteridines vary in their age calibration curves in, common bed (Cimex lectularius) and bat bugs (C. pipistrelli). Here we show that the accumulation of particular pteridines varied between a) different populations and b) rearing temperatures but not c) with the impact of light conditions during extraction or d) the type of blood consumed by the bugs. To optimize the extraction of pteridines and measuring concentrations, we recommend the simultaneous measurement of more than one standard and subsequently to select those that show consistent changes over time to differentiate among age cohorts.

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Determination of testosterone and corticosterone in feathers using liquid chromatography-mass spectrometry

Cited by 8 publications

References 28 publications

Data collection in support of the Endocrine Disruption (ED) assessment for non‐target vertebrates

Data collection in support of the Endocrine Disruption (ED) assessment for non‐target vertebrates

Progress and Challenges in Quantifying Carbonyl-Metabolomic Phenomes with LC-MS/MS

Identification and age-dependence of pteridines in bed bugs (Cimex lectularius) and bat bugs (C. pipistrelli) using liquid chromatography-tandem mass spectrometry

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