Determination of superoxide dismutase and SOD-mimetic activities by a chemical system: Co2/H2O2/lucigenin

Zhidkova, Tatyana V.; Проскурнина, Е. В.; Парфенов, Э. А.; Vladimirov, Yu. A.

doi:10.1007/s00216-011-5070-8

Cited by 12 publications

(5 citation statements)

References 26 publications

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“…(1) The enzyme-like activity of EGO samples in the system of Co(II), H 2 O 2 , and luminol: Besides H 2 O 2 , this system is a source of SAR and hydroxyl radicals [ 67 ] (procedure 9). Previously, we developed a method using this system to assess the activity of superoxide dismutase (SOD) and SOD mimics [ 68 ]. Here, we use luminol as an enhancer sensitive to all formed free radical species, including SAR [ 69 , 70 ], to assess total ROS.…”

Section: Resultsmentioning

confidence: 99%

Green and Sustainable Ultrasound-Assisted Anodic Electrochemical Preparation of Graphene Oxide Dispersions and Their Antioxidant Properties

et al. 2023

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A fast method for preparing aqueous graphene oxide (GO) dispersions by electrochemical oxidation of a graphite anode without preliminary intercalation with oxidizing agents is proposed. Ultrasonic probing was used in the modulation mode of ultrasonic waves (work/rest) for more efficient graphite oxidation–exfoliation. It is shown that the 4/2 s mode of ultrasonic modulation is the most effective due to the probe material’s low corrosion while maintaining the optimum synthesis temperature not exceeding 30–35 °C and achieving the best characteristics of the resulting product. Three cases of anodic oxidation of graphite to obtain graphene oxide were considered: (1) a combined cathode–anode compartment, (2) a split cathode–anode salt-bridged compartment, and (3) separated anode compartment with a 3.5 kDa dialysis membrane. It was determined that the approach to synthesis with a divided cathode–anode compartment makes it possible to obtain GO sheets with fewer defects compared to chemical methods or methods with a combined cathode–anode compartment and makes it possible to control the oxidation degree of the material (C:O ratio) by varying the current density. The prepared samples showed good stability for more than six months. The spectral and morphological characteristics were studied. Using chemiluminometry in the luminol/Co(II)/H2O2 system, the antioxidant properties concerning three key reactive oxygen species (H2O2, superoxide anion radical, and hydroxyl radical) were demonstrated. It was also shown that the prepared GO dispersions do not induce lipid and phospholipid peroxidation.

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Section: Resultsmentioning

confidence: 99%

Green and Sustainable Ultrasound-Assisted Anodic Electrochemical Preparation of Graphene Oxide Dispersions and Their Antioxidant Properties

et al. 2023

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“…There is a large number of various modifications of the chemiluminescent method [ 109 , 110 , 111 , 112 , 113 , 114 ]. The most common modification of the chemiluminescent method for determining total antioxidant capacity is based on the use of luminol (LH 2 ) as an activator of chemiluminescence.…”

Section: Methods To Evaluate Integrated Antioxidant Propertiesmentioning

confidence: 99%

Study of Antioxidant Properties of Agents from the Perspective of Their Action Mechanisms

Ivanova

Газизуллина

2020

Molecules

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The creation and analysis of a large variety of existing methods for the evaluation of integrated antioxidant properties are quite relevant in connection with a range of biological mechanisms of the antioxidants (AO) action. In this work, the existing methods are correlated with mechanisms of antioxidant action. It is shown that the results obtained by various methods are mainly incomparable. This can be connected with the implementation of various mechanisms of antioxidant action in methods. The analysis of the literature data presented in this review indicates the difficulty of creating a universal method and the feasibility of using integrated approaches based on the use of several methods that implement and combine various mechanisms of the chemical conversion of antioxidants. This review describes methods for studying the chelating ability of antioxidants, except for methods based on electron and hydrogen atom transfer reactions, which are currently not widely covered in modern literature. With the description of each mechanism, special attention is paid to electrochemical methods, as the interaction of active oxygen metabolites of radical and non-radical nature with antioxidants has an electron/proton/donor-acceptor nature, which corresponds to the nature of electrochemical methods and suggests that they can be used to study the interaction.

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“…It functions as the first line of defence, and can protect cells from oxidative damage caused by reactive oxygen species by converting the superoxide radical into oxygen and hydrogen peroxide, thereby protecting cells from oxidation (Arockiaraj et al 2014;Lifeng et al 2014). Moreover, SOD can also prevent the superoxide radical from integrating to some other molecules, such as superoxide can react with nitrogen oxide to peroxynitrite, initiating lipid peroxidation (Zhidkova et al 2011).…”

Section: Introductionmentioning

confidence: 99%

Changes in superoxide dismutase activity postpartum from Laoshan goat milk and factors influencing its stability during processing

Chen

et al. 2018

Italian Journal of Animal Science

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The aim of the study was to evaluate superoxide dismutase (SOD) activity from partum to 165 days postpartum of Laoshan dairy goat milk and factors affecting the stability of SOD by the autoxidation of pyrogallol method. The results indicated that the SOD activity in colostrum milk was significantly higher than that in mature milk; and it first decreased and then increased for mature milk. There was a significant decline in SOD activity from 98.54 U/mL at 65 C for 30 min treatment to 39.04 U/mL at 100 C for 5 min treatment. It indicated that SOD activity was not entirely lost, and SOD had strong resistance to high temperature. Maximal SOD activity was recorded at pH 6.5 whereas pH greater or lesser than this resulted in decreased activity. In addition, the change of SOD activity under alkaline conditions was less than that under acidic conditions. SOD activities were 105.12 and 67.08 U/mL when the rotational speeds were 45 and 400 g, respectively. Thus, SOD activity decreased as rotational speed increased. The SOD activity increased when the calcium ion concentration was increased, which ranged from 0.2 to 0.6 mg/ mL. SOD activity was significantly increased by 26% in the first 4 days and decreased after 4 days in the process of fermentation. And the activity of SOD for the fifth day decreased 20% over that of the fourth day. These data comprehensively demonstrate a range of conditions change that significantly affects the integrity, activity and function of SOD in goat milk.

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Determination of superoxide dismutase and SOD-mimetic activities by a chemical system: Co2/H2O2/lucigenin

Cited by 12 publications

References 26 publications

Green and Sustainable Ultrasound-Assisted Anodic Electrochemical Preparation of Graphene Oxide Dispersions and Their Antioxidant Properties

Green and Sustainable Ultrasound-Assisted Anodic Electrochemical Preparation of Graphene Oxide Dispersions and Their Antioxidant Properties

Study of Antioxidant Properties of Agents from the Perspective of Their Action Mechanisms

Changes in superoxide dismutase activity postpartum from Laoshan goat milk and factors influencing its stability during processing

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