Determination of sulfonamides in meat with dummy-template molecularly imprinted polymer-based chemiluminescence sensor

Li, Zhao Bin; Liu, Jing; Liu, Juxiang; Wang, Zhan Hui; Wang, Jian Ping

doi:10.1007/s00216-019-01792-4

Cited by 24 publications

(9 citation statements)

References 35 publications

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“…2 It is well-known that the core of an immunoassay is the used recognition reagent. Up to now, many materials including polyclonal antibodies, 3 monoclonal antibodies, 4,5 IgY, 6 recombinant antibodies, 7,8 aptamers, 9 and molecularly imprinted polymers 10 have been used as recognition reagents for detection of SAs. However, all of these recognition reagents are produced with one molecule as the hapten or template, so they cannot recognize all the SA species.…”

Section: ■ Introductionmentioning

confidence: 99%

Development of a Dihydropteroate Synthase-Based Fluorescence Polarization Assay for Detection of Sulfonamides and Studying Its Recognition Mechanism

Liu

Wang

2021

J. Agric. Food Chem.

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In this study, the dihydropteroate synthase of Staphylococcus aureus was obtained, and its recognition mechanisms for 31 sulfonamide drugs were studied. Results showed that their core structure matched well with the binding pocket of paraaminobenzoic acid, and all the sulfonamide side chains were out of the binding pocket. Hydrogen bonds and hydrophobic interactions were the main intermolecular forces, and the key amino acids were Gly171 and Lys203. The binding sites in sulfonamide molecules were mainly around the para-aminobenzenesulfonamide part. This enzyme was used to develop a fluorescence polarization assay for detection of these drugs in chicken muscles. The change trends of half of inhibition concentrations and crossreactivities for the 31 drugs were identical with the receptor−ligand affinities. The limits of detection were in the range of 2.0−38.5 ng/g, and one assay could be finished within several minutes. Therefore, this method could be used for multiscreening of sulfonamide residues in meat samples.

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Section: ■ Introductionmentioning

confidence: 99%

Development of a Dihydropteroate Synthase-Based Fluorescence Polarization Assay for Detection of Sulfonamides and Studying Its Recognition Mechanism

Liu

Wang

2021

J. Agric. Food Chem.

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“…As shown in Figure 9A, it can be seen that the inhibition curve of the matrix-matched coumaphos was similar to that of the coumaphos standard, indicating a minimal matrix. Therefore, the extracted matrix has no effect on the representative compound, and the subsequent tests in real milk samples can be carried out (Xia et al, 2018;Zhang et al, 2018;Li et al, 2019a).…”

Section: Optimization Of Assay Parametersmentioning

confidence: 99%

“…Molecularly imprinted polymers (MIP), known as plastic antibodies, have been used to synthesize molecular recognition materials with specific adsorption to specific target molecules (Li et al, 2019a). Moreover, MIP synthesis is convenient and efficient, and the synthesized MIP can be reused many times.…”

Section: Introductionmentioning

confidence: 99%

Chemiluminescence sensors based on molecularly imprinted polymers for the determination of organophosphorus in milk

Pan

Liu

et al. 2022

Journal of Dairy Science

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As a food adapted to all kinds of people, milk has a high nutritional value. Because milk is a complex biological matrix, detecting illegal compounds is often difficult. As a common pesticide, organophosphorus (OP) residues caused by nonstandard use may be ignored, which is a threat to milk quality. In this study, using coumaphos as template molecule, the synthesized molecularly imprinted polymer (MIP) can specifically recognize 7 kinds of OP. Then, the MIP was used as an identification element to prepare a chemiluminescence sensor on a 96-well microplate for the determination of OP residues in milk samples. Due to the 4-(imidazol-1-yl)phenol-enhanced luminol-H 2 O 2 system, the sensitivity of the system is very high; the detection limits of 7 OP including coumaphos, fenthion, chlorpyrifos, parathion, diazinon, fenchlorphos, and fenitrothion were 1 to 3 pg/mL, and the half maximal inhibitory concentrations were 1 to 20 ng/mL. The intraday recoveries of 7 OP were in the range of 86.1 to 86.5%, and the interday recoveries were in the range of 83.6 to 94.2%. Furthermore, the sensor can be reused up to 5 times. Therefore, the MIP-based chemiluminescence sensor can be used as a routine tool to detect OP residues in milk samples.

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“…MD-based investigations of other aspects of the pre-polymerization stage include studies of the structural stability of protein epitopes for template screening [ 414 , 415 ], mapping potential monomer interaction sites of a protein target, followed by docking of acrylamide-derived monomers and post-docking interaction energy calculations [ 416 ], studies of template interactions with Dengue virus as a support matrix to create larger binding sites [ 417 ], a series of reports attempting to correlate structural and physical properties of dummy templates and ligands with rebinding properties [ 418 , 419 , 420 , 421 , 422 , 423 , 424 , 425 , 426 ] and coarse-grained simulations studying the effect of composition on material properties and template interaction [ 427 ]. Additionally, large-scale MD simulations were performed in an attempt to mimic chromatography in a virtual capillary [ 428 ].…”

Section: The Pre-polymerization Stagementioning

confidence: 99%

The Use of Computational Methods for the Development of Molecularly Imprinted Polymers

et al. 2021

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Recent years have witnessed a dramatic increase in the use of theoretical and computational approaches in the study and development of molecular imprinting systems. These tools are being used to either improve understanding of the mechanisms underlying the function of molecular imprinting systems or for the design of new systems. Here, we present an overview of the literature describing the application of theoretical and computational techniques to the different stages of the molecular imprinting process (pre-polymerization mixture, polymerization process and ligand–molecularly imprinted polymer rebinding), along with an analysis of trends within and the current status of this aspect of the molecular imprinting field.

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Determination of sulfonamides in meat with dummy-template molecularly imprinted polymer-based chemiluminescence sensor

Cited by 24 publications

References 35 publications

Development of a Dihydropteroate Synthase-Based Fluorescence Polarization Assay for Detection of Sulfonamides and Studying Its Recognition Mechanism

Development of a Dihydropteroate Synthase-Based Fluorescence Polarization Assay for Detection of Sulfonamides and Studying Its Recognition Mechanism

Chemiluminescence sensors based on molecularly imprinted polymers for the determination of organophosphorus in milk

The Use of Computational Methods for the Development of Molecularly Imprinted Polymers

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