Determination of Sulfide in Spring and Wastewater by a New Kinetic Spectrophotometric Method

Emami, Marjan; Mousavi, Mir F.; Barzegar, Mohsen

doi:10.1002/jccs.200400078

Cited by 5 publications

(1 citation statement)

References 26 publications

(12 reference statements)

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“…A stock solution of 10000 ppm to be utilized was prepared by dissolving 7.5 g (wv -1 ) of Sodium sulfide (Na 2 S.9H 2 O), in 1000 ml. From the stock solution, standard working simulated solutions of 200, 300 and 500 ppm were made through appropriate serial dilution (Emami, et al 2004). To each of the three out of four Erlenmeyer flasks containing 150 ml NB and 20 ml inoculum in a different concentration of sulfide in the range of 200, 300 and 500 ppm was added.…”

Section: Biodegradation Studiesmentioning

confidence: 99%

Biological Sulfide Oxidation and Its Impact on Cell Biomass Synthesis by Mesophilic Bacterium Bacillus Cerues (Atcc 14579)

Ahmad¹,

Aziz²,

Khushairi³

et al. 2017

JCEIB

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Inhibitory effects of hydrogen sulfide could either be due to toxicity to living tissue or precipitation of sulfide salts which inhibit its utilization for cell growth. Growth, is undoubtedly affected by certain conditions, among which are nutrient types and availability, as well as the operational parameters of the fermenting medium. In this study, Bacillus cereus (ATCC 14579) was tested for potential sulfide biodegradation in a suspended growth medium of orbital shake flasks using a single milieu composition under defined operational parameters. Growth and sulfide oxidation efficiency were measured spectrophotometrically under optimum physical conditions of pH, temperature, acclimatization time and agitation. Sulfide reduction was overwhelmingly recorded at three different sulfide loading rates of 200 ppm S2- L-1 d- 1, 300 ppm S2- L-1 d-1 and 500 ppm S2- L-1 d-1 with corresponding appreciable cell growth measured at OD600 nm. Results indicated that it was possible to realise sulfide removal efficiency of 95% to 99% using this strain in an orbital shake flask within 24 hrs, as well as 65% to 78% within the first 6 hrs of inoculation. Overall, sulfide wasreduced by 95% in 200 ppm and 300 ppm, while 99% in 500 ppm, respectively. While, the corresponding exponential cell growth recorded was 3.91, 3.80 and 3.61 in 200 ppm, 300 ppm and 500 ppm, respectively. This also translate to cell biomass synthesis (cell dry weight) of 0.61 g/L, 0.58 g/L and 0.50 g/L in 200 ppm, 500 ppm and 300 ppm. Based on this finding, it was clear that this inoculum can utilize different sulfide concentration for growth and biosynthesis; thus can be employed to treat sulfide contaminated wastewater in a suspended growth form under simple nutrient composition and operational conditions.

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Section: Biodegradation Studiesmentioning

confidence: 99%

Biological Sulfide Oxidation and Its Impact on Cell Biomass Synthesis by Mesophilic Bacterium Bacillus Cerues (Atcc 14579)

Ahmad¹,

Aziz²,

Khushairi³

et al. 2017

JCEIB

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A comparison between determination of trace amounts of sulfide in the presence and absence of micelle particles in natural waters (Qazvin, Iran): a kinetic spectrophotometric approach

Alizadeh

Mahjoub

2015

Environ Monit Assess

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A new sensitive kinetic spectrophotometric method described for the determination of trace amounts of sulfides based on the addition reaction of sulfide ions with malachite green has been investigated in aqueous and micellar media at 25 °C. The variables affecting the rate of the reaction were investigated, and the optimum conditions were established. Under the optimum experimental conditions, decreases in the absorbance of malachite green at 615 nm in the absence and 630 nm in the presence of micelle particles, their λ max, were proportional to the concentrations of sulfide ions at the first 15 and 25 s from initiation of the reaction. The working curve was linear over the concentration range 50-1200 ng mL(-1) of sulfide ions with a fixed time method at the first 15 and 25 s from initiation of the reaction in aqueous medium and 25-1750 ng mL(-1) with a fixed time method at the first 15 s and 25-1500 ng mL(-1) for primitive 25 s in micellar medium. For the proposed kinetic method, the experimental and theoretical limit of detection (LOD) and limit of quantification (LOQ) in the presence and absence of micelle particles were obtained and tabulated at Δt = 15 and 25 s. The effective range concentration was achieved from the plot of Ringbom in both media and reported. Different surfactants, such as nonionic surfactant (Triton-X100), anionic surfactant sodium dodecyl sulfate (SDS), and cationic surfactant cetyltrimethylammonium bromide (CTAB) and cetylpyridinium chloride (CPC), were investigated and Triton X-100 selected as a suitable surfactant. To valuable vision into the reaction pathways, pseudo-first-order condition was applied and different kinetic parameters like ΔG (≠), ΔE (≠), ΔS (≠), and ΔH (≠) computed. The 2:1 stoichiometry of malachite green to sulfide ions was indicated by the results of mole ratio and Job's method of continuous variation. The effect of different environments on the interfering of various ions on sulfide determination with the suggested method was studied, and final results have been presented. To investigate the usefulness of the different media of the proposed method, sulfide ions were determined in natural waters such as river and spring samples without any purification or using masking reagents.

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Selective spectrofluorimetric determination of sulfide ion using manganese doped ZnS quantum dots as luminescent probe

Rajabi

Shamsipur

Khosravi

et al. 2013

Spectrochimica Acta Part A: Molecular and Biomolecular Spectros

109

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Determination of Sulfide in Spring and Wastewater by a New Kinetic Spectrophotometric Method

Cited by 5 publications

References 26 publications

Biological Sulfide Oxidation and Its Impact on Cell Biomass Synthesis by Mesophilic Bacterium Bacillus Cerues (Atcc 14579)

Biological Sulfide Oxidation and Its Impact on Cell Biomass Synthesis by Mesophilic Bacterium Bacillus Cerues (Atcc 14579)

A comparison between determination of trace amounts of sulfide in the presence and absence of micelle particles in natural waters (Qazvin, Iran): a kinetic spectrophotometric approach

Selective spectrofluorimetric determination of sulfide ion using manganese doped ZnS quantum dots as luminescent probe

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