Determination of sulfa antibiotic residues in river and particulate matter by field‐amplified sample injection‐capillary zone electrophoresis

Abstract

In the present research, field-amplified sample injection-CZE (FASI-CZE) coupled with a diode array detector was established to determine trace level sulfa antibiotic. Sulfathiazole, sulfadiazine, sulfamethazine, sulfadimethoxine, sulfamethoxazole, and sulfisoxazole were selected as analytes for the experiments. The background electrolyte solution consisted of 70.0 mmol/L borax and 60.0 mmol/L boric acid (including 10% methanol, pH 9.1). The plug was 2.5 mmol/L borax, which was injected into the capillary at a pressure of 0.5 psi for 5 s. Then the sample was injected into the capillary at an injection voltage of-10 kV for 20 s. The electrophoretic separation was carried out under a voltage of +19 kV. The capillary temperature was maintained at 20˚C throughout the analysis, and six sulfonamides were completely separated within 35 min. Compared with pressure injection-CZE, the sensitivity of FASI-CZE was increased by 6.25-10.0 times, and the LODs were reduced from 0.2-0.5 to 0.02-0.05 μg/mL. The method was applied to the determination of sulfonamides in river water and particulate matter samples. The recoveries were 78.59-106.59%. The intraday and interday precisions were 2.89-7.35% and 2.77-7.09%, respectively. This provides a simpler and faster method for the analysis of sulfa antibiotic residues in environmental samples.

Abundance and Dynamic Distribution of Antibiotic Resistance Genes in the Environment Surrounding a Veterinary Antibiotic Manufacturing Site

“…With the influence of temperature and pH of the environmental media, natural photosensitizers, etc., antibiotic drugs are prone to hydrolysis or photolysis [49]. One probable reason why antibiotic residues were not detected in the environmental media is that they had been Our previous studies [46,47] have shown that sulfonamides and β-lactams antibiotic residues were not detected in Min-xin river water and soil along the river [48]. This is likely due to that antibiotic drugs can be degraded in the environment.…”

“…Our previous studies [ 46 , 47 ] have shown that sulfonamides and β-lactams antibiotic residues were not detected in Min-xin river water and soil along the river [ 48 ]. This is likely due to that antibiotic drugs can be degraded in the environment.…”

“…Residues of sulfa antibiotic (sulfathiazole, sulfadiazine, sulfamethazine, sulfadimethoxine, sulfamethoxazole, and sulfisoxazole) in Min-xin river water were determined by field-amplified sample injection–CZE (FASI–CZE) coupled with a diode array detector, as previously reported [ 46 ]. The conditions for capillary electrophoresis analysis were as follows: the background electrolyte solution consisted of 70.0 mmol·L −1 borax and 60.0 mmol·L −1 boric acid (including 10% methanol, pH 9.1); the plug was 2.5 mmol·L −1 borax, which was injected into the capillary at a pressure of 0.5 psi for 5 s; then the sample was injected into the capillary at an injection voltage of −10 kV for 20 s; the electrophoretic separation was carried out under a voltage of +19 kV.…”

Multisensory Systems Based on Perfluorosulfonic Acid Membranes Modified with Polyaniline and PEDOT for Multicomponent Analysis of Sulfacetamide Pharmaceuticals

“…This dictates the need for its simultaneous determination with other members of this class. The techniques of HPLC liquid chromatography [5], thin layer chromatography [5], capillary electrochromatography [6], electrophoresis [7,8], and spectrophotometry [9] are used for the determination of 4-aminobenzenesulfonic acid derivatives. For the analysis of media with complex matrixes, including those containing several 4-aminobenzenesulfonic acid derivatives, hybrid methods combining extraction and chromatography with tandem mass-spectrometric [10][11][12] Polymers 2022, 14, 2545 2 of 21 or UV-detectors [13], extraction and electrophoresis with UV-detection [14], extraction and UV-Visible spectrometry [15,16] are commonly used.…”

Preparation of Fe/Ni Bimetallic Oxide Porous Graphene Composite Materials for Efficient Adsorption and Removal of Sulfonamides

“…Sulfonamides (SAs) are a kind of artificial synthetic antibiotics, which are mainly used to effectively prevent and treat various infectious diseases in human and animals. − However, the excessive or long-term use of SAs will lead to their wide spread emission in the environment, which will increase the resistance of aquatic microorganisms and then affect the human body through the food chain. , For example, causing the body flora imbalance, digestive system dysfunction, and even death. , This seriously threatens human health and the ecosystem. At present, the removal of antibiotics has become a worldwide environmental health problem.…”