Determination of Sugars on Paper Chromatograms with <i>p</i>-Anisidine Hydrochloride

Pridham, J. B.

doi:10.1021/ac60120a041

Cited by 135 publications

(29 citation statements)

References 3 publications

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“…Compounds were hydrolyzed with 7% sulfuric acid. The hydrolyzable sugars were identified by paper chromatography according to the method of Pridham (13). The C-glycosidic moieties of some compounds have not been completely characterized.…”

Section: Methodsmentioning

confidence: 99%

The Origin of Novel Flavonoids in Phlox Allotetraploids

Levy

Levin

1971

Proc. Natl. Acad. Sci. U.S.A.

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The flavonoids of two diploid species of Phlox and two of their allotetraploid derivatives were characterized. The tetraploids accumulate five flavonoids not observed in the parental species. Generally, novel compounds are less highly glycosidated than, but otherwise similar to, parental compounds that they partially or totally replace. We propose that hybridity and polyploidy have repressed or suppressed the activity of certain ancestral genes responsible for the production of glycosidating enzymes. Accordingly, the novel compounds are merely the accumulated or modified precursors of parental compounds.

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Section: Methodsmentioning

confidence: 99%

The Origin of Novel Flavonoids in Phlox Allotetraploids

Levy

Levin

1971

Proc. Natl. Acad. Sci. U.S.A.

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“…In the complex, glucose is bound to peptide and glycosidically to carotenoid since unhydrolysed material is anisidine phthalate negative. The amino acid analysis showed a wider variety of amino acids with less exact molar ratios compared to the carotenoid-glucopeptide of Sarcina flava (Thirkell & Hunter, 1969). This bound carotenoid represents one form in which pigment exists in the membrane where all such pigment is localized.…”

Section: Discussionmentioning

confidence: 99%

“…An absorption spectrum was recorded from 210 to 600 nm. and the following were also determined: ( a ) dry weight and ash, (b) protein by the Folin-Lowry method using serum albumin as standard or by the quantitative ninhydrin method (Moore & Stein, ~954) with norleucine as standard, (c) (Pridham, 1956) or 2 % (w/v) ninhydrin in acetone. …”

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confidence: 99%

“…Homogeneity was tested by examining (a) sedimentation patterns in a Beckman analytical ultracentrifuge, (b) resolution on columns of (i) various grades of Sephadex, (ii) Biogel A15 (4 % agarose), and (c) resolution by disc-gel electrophoresis on 7.5 % cyanogiini C40 gels at pH 4.7. An estimate of the molecular weight was obtained by (a) (Pridham, 1956) or 2 % (w/v) ninhydrin in acetone.…”

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confidence: 99%

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A water-soluble Carotenoid-glycopeptide from Sarcina morrhuae

Thirkell

Hunter

1970

Journal of General Microbiology

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Free pigments have been isolated from Sarcina morrhuae by Nandy & Sen ( I 967), and their results have been confirmed. When no more pigment could be extracted, the bacterial remnants were still pigmented. The present work was undertaken to see how the remaining pigment was bound.and 2-5 % agar no. 3 at 34' for 3 weeks, Pigment was removed by repeated ultrasonication in methanol and then repeated saponification with 10 "/, (wiv) methanolic KOH (at room temperature under an inert atmosphere and in the dark). When no further pigment was extracted, the residue was stirred at 40' in distilled water and a redorange solution recovered.The solution was desalted using Sephadex C75 followed by ultrafiltration in an Amicon high pressure cell. Homogeneity was tested by examining (a) sedimentation patterns in a Beckman analytical ultracentrifuge, (b) resolution on columns of (i) various grades of Sephadex, (ii) Biogel A15 (4 % agarose), and (c) resolution by disc-gel electrophoresis on 7.5 % cyanogiini C40 gels at pH 4.7. An estimate of the molecular weight was obtained by (a) behaviour on dialysis (molecular weight cut-off ~O O O ) , (b) retention on columns of various Sephadexes and on 4 "/o agarose (standardized for both Stokes radius and molecular weight). An absorption spectrum was recorded from 210 to 600 nm. and the following were also determined: ( a ) dry weight and ash, (b) protein by the Folin-Lowry method using serum albumin as standard or by the quantitative ninhydrin method (Moore & Stein, ~954) with norleucine as standard, (c) (Pridham, 1956) or 2 % (w/v) ninhydrin in acetone.

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“…Accordingly, those fractions found to consist chiefly of one or another sugar were selected and the products characterized as described below. The proportion of the pentoses present in each fraction was approximated visually from the intensity and area of the spots developed on the chromatograms and electrophoretograms with p-anisidine hydrochloride spray, a spray suited to the estimation of sugars (15). Related to the known weight of each fraction and the amount of arabinose separated earlier as the crystalline glycoside, these evaluations suggested that the ratio of arabinosexylose-lyxose-ribose in the reaction mixture was about Methyl a-D-1yxopyranoside.-The mixture of glycosides obtained by isomerization of methyl arabinoside was chromatographed on a cellulose powder column using solvent E, a series of fractions being collected and examined chromatographically.…”

Section: Identijication Of the Reaction Productsmentioning

confidence: 99%

HYDROGENOLYSIS OF CARBOHYDRATES: V. ISOMERIZATION OF METHYL β-L-ARABOPYRANOSIDE

Perlin¹,

Rudloff²,

Tulloch³

1958

Can. J. Chem.

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Ireatment of methyl 8-L-arabopyranoside under hydrogenolytic conditions with copper chromium oxide catalyst induces extensive isonierization. Products formed include glycosides of D-ribose, D-xyIose, D-lyxose, and L-lyxose which together represent about a n 80-90% conversion of starting material. Isolation of these compounds shows that carbons 2 , 3 , and 4 undergo isorl~erization, but no evidence has been obtained of anomerization. These findings possibly explain the formation of an optically inactive mixture of cis-and trans-tetrahydropyran-3,-1-diol u~lder slightly more drastic reaction conditions.

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Determination of Sugars on Paper Chromatograms with p-Anisidine Hydrochloride

Cited by 135 publications

References 3 publications

The Origin of Novel Flavonoids in Phlox Allotetraploids

The Origin of Novel Flavonoids in Phlox Allotetraploids

A water-soluble Carotenoid-glycopeptide from Sarcina morrhuae

HYDROGENOLYSIS OF CARBOHYDRATES: V. ISOMERIZATION OF METHYL β-L-ARABOPYRANOSIDE

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