Determination of sugars by liquid chromatography and amperometric detection with a cuprous oxide modified electrode

Abstract

Determination of sugars by liquid chromatography and amperometric detection with a cuprous oxide modified electrode Huang, X.; Pot, J.J.; Kok, W.T. Disclaimer/Complaints regulationsIf you believe that digital publication of certain material infringes any of your rights or (privacy) interests, please let the Library know, stating your reasons. In case of a legitimate complaint, the Library will make the material inaccessible and/or remove it from the website. Please Ask the Library: http://uba.uva.nl/en/contact, or a letter to: Library of the University of Amsterdam, Secretariat, Singel 425, 1012 WP Amsterdam, The Netherlands. You will be contacted as soon as possible. SummaryA chemically modified electrode was applied as a working electrode for the amperometric detection of sugars in liquid chromatography. A mixture of cuprous oxide and conductive carbon cement was used as electrode material. Cuprous oxide acted as a catalyst for the oxidation of sugars. Cyclic voltammetry was used to examine the oxidation of the sugars at the electrode surface. The stability and sensitivity of a cuprous oxide/conductive carbon cement (20/80 %, w/w) electrode were tested in flow injection analysis experiments. For liquid chromatography, sugars were separated on an ion-exclusion column with 0.01 M H2SO4 as mobile phase. After the separation, 0.2 M NaOH was added post-column and the sugars were determined at 550 mV vs. Ag/AgC1. Calibration was linear in the range of 10 -6. 10 -3 M with limits of detection of approximately 1 9 10 .6 M. The application of the modified electrode for the determination of sugars in fruit beverages and dairy products following chromatographic separation is shown.

Amperometric Detection of Carbohydrates by Capillary Electrophoresis with a Cuprous Oxide Modified Sol-Gel Carbon Composite Electrode

“…With an applied potential of 0.65 V, the limits of detection (LOD), based on a SyN 3, were below 1.3 mM. Detection limits with this electrode are comparable with those obtained with other electrodes under similar conditions [7,13]. The sensitivity of CCE is comparable to the copper electrode [4].…”

“…The choice of the catalysts has been mainly focused on transition metal oxides and complexes [7, 9±11]. Among the catalysts investigated, cuprous oxide has appeared to possess high catalytic activity for the oxidation of carbohydrates [12,13].…”