Determination of succinylacetone in dried blood spots and liquid urine as a dansylhydrazone by liquid chromatography tandem mass spectrometry

Al-Dirbashi, Osama Y.; Rashed, Mohamed S.; Brink, H.J. ten; Jakobs, Cornelis; Filimban, Najlaa; Al-Ahaidib, Lujane Y.; Jacob, Mohan V.; Al-Sayed, Moeen; Al‐Hassnan, Zuhair N.; Faqeih, Eissa

doi:10.1016/j.jchromb.2005.12.010

Cited by 36 publications

(34 citation statements)

References 22 publications

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“…The extracts can be directly injected onto the LC-MS/MS system for analysis, or dried for reconstitution using an MS-friendly solvent prior to analysis. Derivatization is one of the common approaches for enhanced MS/MS detection sensitivity for trace analytes (Al-Dirbashi et al, 2006, 2008Eyles et al, 2009;Higashi et al, 2008;Lai et al, 2001;Magera et al, 2006). However, one should be aware that derivatization might be a source of assay error due to unwanted reactions, e.g.…”

Section: Dbs Sample Extractionmentioning

confidence: 99%

Dried blood spot sampling in combination with LC‐MS/MS for quantitative analysis of small molecules

Li¹,

Tse²

2009

Biomedical Chromatography

530

425

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The collection of whole blood samples on paper, known as dried blood spot (DBS), dates back to the early 1960s in newborn screening for inherited metabolic disorders. DBS off ers a number of advantages over conventional blood collection. As a less invasive sampling method, DBS off ers simpler sample collection and storage and easier transfer, with reduced infection risk of various pathogens, and requires a smaller blood volume. To date, DBS-LC-MS/MS has emerged as an important method for quantitative analysis of small molecules. Despite the increasing popularity of DBS-LC-MS/MS, the method has its limitations in assay sensitivity due to the small sample size. Sample quality is often a concern. Systematic assessment on the potential impact of various blood sample properties on accurate quantifi cation of analyte of interest is necessary. Whereas most analytes may be stable on DBS, unstable compounds present another challenge for DBS as enzyme inhibitors cannot be conveniently mixed during sample collection. Improvements on the chemistry of DBS card are desirable. In addition to capturing many representative DBS-LS-MS/MS applications, this review highlights some important aspects of developing and validating a rugged DBS-LC-MS/MS method for quantitative analysis of small molecules along with DBS sample collection, processing and storage.

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Section: Dbs Sample Extractionmentioning

confidence: 99%

Dried blood spot sampling in combination with LC‐MS/MS for quantitative analysis of small molecules

Li¹,

Tse²

2009

Biomedical Chromatography

530

425

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“…SA was from Sigma-Aldrich (St Louis, MO, USA) and 13 C 4 -SA was synthesized according to the published procedure (Al-Dirbashi et al, 2006). Dansylhydrazine (Dns-H) was purchased from Fluka Chemie (Buchs, Switzerland).…”

Section: Methodsmentioning

confidence: 99%

“…SA was also determined in specimens from patients with established HT1 (n = 11). Sample pretreatment was exactly as described in our previous report (Al-Dirbashi et al, 2006). In brief, SA eluted from a single 3/16 inch disk of the DBS using HCl (0.5 mL, 0.5 mol/L) was extracted into 1 mL of ethylacetate and the solvent was evaporated.…”

Section: Methodsmentioning

confidence: 99%

“…To achieve this objective, we and others have recently reported tandem mass spectrometry (MS/MS) or high-performance liquid chromatography tandem mass spectrometry (HPLC-MS/MS) methods for SA quantification in dried blood spots (DBS), the preferred and commonly received specimen in newborn screening laboratories (Allard et al, 2004;Al-Dirbashi et al, 2006;Magera et al, 2006;Johnson et al, 2007). In this work, we describe a further improvement in the chromatographic process compared with our previous method (Al-Dirbashi et al, 2006) that allowed for a fast one-minute assay of SA in a biological matrix with no compromise on the specificity and sensitivity. This was achieved by utilizing a short narrow-bore column packed with very small particles (1.7 μm) in combination with a mobile phase delivery system that operates at high back pressures, a recent technology termed ultra-performance liquid chromatography (UPLC).…”

Section: Introductionmentioning

confidence: 99%

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Improved method to determine succinylacetone in dried blood spots for diagnosis of tyrosinemia type 1 using UPLC‐MS/MS

Al-Dirbashi

Rashed

Jacob

et al. 2008

Biomedical Chromatography

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We describe an improved diagnostic method for tyrosinemia type 1 based on quantifying succinylacetone in dried blood spots by ultra-performance liquid chromatography tandem mass spectrometry. Succinylacetone extracted from a single 3/16 inch disk of specimen collection paper containing a dried blood spot was derivatized with dansylhydrazine, separated on an Acquity UPLC BEH C(18) column (2.1 x 50 mm, 1.7 microm) and detected by electrospray ionization tandem mass spectrometry. Succinylacetone derivative eluted at 0.6 min with a complete run time of 1 min. Using a 13C4 labeled succinylacetone as an internal standard, the calibration plot was linear up to 100 micromol/L with a detection limit (S/N = 3) of 0.2 micromol/L. Intra-day (n = 13) and inter-day (n = 10) variations were better than 10%. The cutoff level of succinylacetone in dried blood spots from healthy infants obtained by the current method was 0.63 micromol/L (n = 151). In dried blood spots from patients with established tyrosinemia type 1 (n = 11), concentration of succinylacetone was 6.4-30.8 micromol/L.

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“…Because of nonspecific elevations of tyrosine levels in transient tyrosinemia, and methionine in hepatopathies or in babies on hypoallergenic alimentation, test specificity is also limited. Attempts have been made to introduce succinyl acetone and homocysteine in routine screening [68,69,184,185]. However, these require either preparation of separate samples (succinyl acetone) or extra analytical procedures (homocysteine) and do not ideally meet economic screening criteria.…”

Section: Tyrosinemia Type I and Homocystinuriamentioning

confidence: 99%

Newborn screening for inborn errors of metabolism and endocrinopathies: an update

Fingerhut

Olgemöller

2008

Anal Bioanal Chem

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Determination of succinylacetone in dried blood spots and liquid urine as a dansylhydrazone by liquid chromatography tandem mass spectrometry

Cited by 36 publications

References 22 publications

Dried blood spot sampling in combination with LC‐MS/MS for quantitative analysis of small molecules

Dried blood spot sampling in combination with LC‐MS/MS for quantitative analysis of small molecules

Improved method to determine succinylacetone in dried blood spots for diagnosis of tyrosinemia type 1 using UPLC‐MS/MS

Newborn screening for inborn errors of metabolism and endocrinopathies: an update

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