Determination of Substrate Preference in Phosphatidylserine Decarboxylation by Liquid Chromatography–Electrospray Ionization Mass Spectrometry

Kevala, Jillonne H.; Kim, Hee‐Yong

doi:10.1006/abio.2001.5076

Cited by 32 publications

(27 citation statements)

References 29 publications

(19 reference statements)

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“…So the substrate specificity of the decarboxylase explains at least in part the preferential synthesis of PtdEtn species with long-chain polyunsaturated fatty acids at the sn-2 position. This observation is in contrast to earlier reports, where no clear preference of PSD for polyunsaturated fatty acids was detected in in vitro studies (35,36). We have no ready explanation for this discrepancy except that different tissue, cell lines, and methodologies were used.…”

Section: Preferential Synthesis Of Polyunsaturated Ptdetn Species Viacontrasting

confidence: 99%

The CDP-ethanolamine Pathway and Phosphatidylserine Decarboxylation Generate Different Phosphatidylethanolamine Molecular Species

Bleijerveld

Brouwers

Vaandrager

et al. 2007

Journal of Biological Chemistry

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In mammalian cells, phosphatidylethanolamine (PtdEtn) is mainly synthesized via the CDP-ethanolamine (Kennedy) pathway and by decarboxylation of phosphatidylserine (PtdSer). However, the extent to which these two pathways contribute to overall PtdEtn synthesis both quantitatively and qualitatively is still not clear. To assess their contributions, PtdEtn species synthesized by the two routes were labeled with pathway-specific stable isotope precursors,

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Section: Preferential Synthesis Of Polyunsaturated Ptdetn Species Viacontrasting

confidence: 99%

The CDP-ethanolamine Pathway and Phosphatidylserine Decarboxylation Generate Different Phosphatidylethanolamine Molecular Species

Bleijerveld

Brouwers

Vaandrager

et al. 2007

Journal of Biological Chemistry

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“…Fluidity of the membrane or membrane curvature affected by PE and CL are parameters that may influence biophysical properties of the membrane environment. Also the acyl chain length of specific phospholipid species could have an effect (Dolis et al 1996;Heikinheimo and Somerharju 1998;Kevala and Kim 2001). A balanced concentration of phospholipids, sterols, and sphingolipids appears to be important for membrane fusion or fission.…”

Section: Discussionmentioning

confidence: 99%

Lipid Transport between the Endoplasmic Reticulum and Mitochondria

Flis

Daum

2013

Cold Spring Harbor Perspectives in Biology

165

127

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Mitochondria are partially autonomous organelles that depend on the import of certain proteins and lipids to maintain cell survival and membrane formation. Although phosphatidylglycerol, cardiolipin, and phosphatidylethanolamine are synthesized by mitochondrial enzymes, phosphatidylcholine, phosphatidylinositol, phosphatidylserine, and sterols need to be imported from other organelles. The origin of most lipids imported into mitochondria is the endoplasmic reticulum, which requires interaction of these two subcellular compartments. Recently, protein complexes that are involved in membrane contact between endoplasmic reticulum and mitochondria were identified, but their role in lipid transport is still unclear. In the present review, we describe components involved in lipid translocation between the endoplasmic reticulum and mitochondria and discuss functional as well as regulatory aspects that are important for lipid homeostasis.

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“…This methodology has recently been applied to broader biochemical studies (e.g., Hamilton et al, 2000;Kevala & Kim, 2001;Kim, Akbar, & Kim, 2001;Igbavboa et al, 2002;Murthy et al, 2002). Other investigators have applied the HPLC-ESI/MS or ESI/MS/MS to quantitate a specific class or group of lipid molecular species.…”

Section: Quantitation Of Cellular Lipidome By a Hplc-esi/ms Approachmentioning

confidence: 98%

Shotgun lipidomics: Electrospray ionization mass spectrometric analysis and quantitation of cellular lipidomes directly from crude extracts of biological samples

Han

Gross

2004

Mass Spectrometry Reviews

1,038

1,131

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Lipidomics, after genomics and proteomics, is a newly and rapidly expanding research field that studies cellular lipidomes and the organizational hierarchy of lipid and protein constituents mediating life processes. Lipidomics is greatly facilitated by recent advances in, and novel applications of, electrospray ionization mass spectrometry (ESI/MS). In this review, we will focus on the advances in ESI/MS, which have facilitated the development of shotgun lipidomics and the utility of intrasource separation as an enabling strategy for utilization of 2D mass spectrometry in shotgun lipidomics of biological samples. The principles and experimental details of the intrasource separation approach will be extensively discussed. Other ESI/MS approaches towards the quantitative analyses of global cellular lipidomes directly from crude lipid extracts of biological samples will also be reviewed and compared. Multiple examples of lipidomic analyses from crude lipid extracts employing these approaches will be given to show the power of ESI/MS techniques in lipidomics. Currently, modern society is plagued by the sequelae of lipid-related diseases. It is our hope that the integration of these advances in multiple disciplines will catalyze the development of lipidomics, and such development will lead to improvements in diagnostics and therapeutics, which will ultimately result in the extended longevity and an improved quality of life for humankind.

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Determination of Substrate Preference in Phosphatidylserine Decarboxylation by Liquid Chromatography–Electrospray Ionization Mass Spectrometry

Cited by 32 publications

References 29 publications

The CDP-ethanolamine Pathway and Phosphatidylserine Decarboxylation Generate Different Phosphatidylethanolamine Molecular Species

The CDP-ethanolamine Pathway and Phosphatidylserine Decarboxylation Generate Different Phosphatidylethanolamine Molecular Species

Lipid Transport between the Endoplasmic Reticulum and Mitochondria

Shotgun lipidomics: Electrospray ionization mass spectrometric analysis and quantitation of cellular lipidomes directly from crude extracts of biological samples

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