Determination of storage solutions influence on human enamel by Raman spectroscopy

Buchwald, Tomasz; Okulus, Zuzanna

doi:10.1016/j.vibspec.2018.04.003

Cited by 7 publications

(7 citation statements)

References 21 publications

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“…The use of storage solutions such as formalin, sodium azide, or thymol-based mediums has been reported to affect the DDPen measurements [ 34 , 38 ]. The values of fluorescence emission intensity decrease with time of immersion in all storage solutions, except for artificial saliva and glutaraldehyde [ 39 ]. The storage solutions tend to remove the organic compounds from the enamel surface.…”

Section: Discussionmentioning

confidence: 99%

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Effectiveness of Visual-Tactile Examination and DIAGNOdent Pen in Detecting Early Enamel Caries and Its Remineralisation: An In Vitro Study

Rashid

Karobari

Halim

et al. 2022

BioMed Research International

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Background. The caries preventive effect of Colgate Duraphat® and GC Tooth Mousse Plus® has been widely studied, but the remineralisation potential of initial occlusal caries using these two remineralisation materials remains unclear. Aim. This study is aimed at evaluating and comparing the remineralisation of early enamel caries on the occlusal surface of permanent posterior teeth using ICDAS II caries scoring system and DIAGNOdent Pen (DDPen) after remineralisation with Colgate Duraphat® and GC Tooth Mousse Plus®. Materials and Methods. Extracted posterior teeth ( N = 120 ) with incipient occlusal caries were included in this study. The occlusal surface of each tooth was scored using DDPen and ICDAS II scoring before remineralisation. Then, remineralisation of the teeth of the experimental group was carried out using either CPP-ACP-F or fluoride varnish. After the remineralisation procedures, the occlusal surface of each tooth was again scored using DDPen and ICDAS II scoring. The teeth were then fixed in dental stone blocks and sectioned longitudinally for histological examination using a stereomicroscope. Statistical analysis was performed to calculate the sensitivity and specificity of DDPen and ICDAS II to detect remineralisation and compare with the gold standard histological examination. Results. According to ICDAS-II scores, a significant difference was noted in GC Tooth Mousse Plus® and Duraphat® study samples, whereas the difference between the pre-and post-remineralisation of the control group was not significant. According to the DDPen score criteria, a statistically significant difference was noted among all study groups; however, a greater significance level was noted in the GC Tooth Mousse Plus® and Duraphat® study samples compared with the control group. The Spearman’s rank correlation of ICDAS-II and DDPen with Downer’s histological score (gold standard) revealed a higher association of DDPen score (.738) as compared to ICDAS-II scores (.430). Conclusion. The study concluded that both ICDAS II and DDPen could detect remineralisation of early enamel occlusal caries. DDPen was more sensitive than ICDAS-II to detect remineralisation compared with the Downers histological scores.

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Section: Discussionmentioning

confidence: 99%

“…The storage solutions tend to remove the organic compounds from the enamel surface. It is important to note that distilled water does not remove the organic compounds after 30 days of immersion [ 39 ]. Immersion in 0.1% thymol solution has shown a decline compared to the laser fluorescence values [ 40 ].…”

Section: Discussionmentioning

confidence: 99%

Effectiveness of Visual-Tactile Examination and DIAGNOdent Pen in Detecting Early Enamel Caries and Its Remineralisation: An In Vitro Study

Rashid

Karobari

Halim

et al. 2022

BioMed Research International

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“…Cabe notar que previamente se debe hacer una desinfección mecánica y química efectiva de la raíz y la cámara pulpar de los especímenes, manteniendo la corona tan intacta como sea posible (8) y teniendo en cuenta que el tiempo de almacenamiento no debe exceder más de 4 semanas (20,21). Tiene una gran ventaja frente a los otros medios y es que no afecta la fluorescencia (11).…”

Section: Discussionunclassified

“…5. Almacenar en saliva artificial específica para este uso baja en calcio y fósforo para evitar remineralización inducida 4°C (10) por un tiempo 3 a 4 semanas (20,21) ó agua destilada 4°C (10) sin recambio hasta 4 semanas (11).…”

Section: Odontologíaunclassified

“…Esto representa un problema, ya que sí los dientes sobre los que se van estudiar color están muy blancos, seguramente no presentarán grandes diferencias postratamiento, lo que complica la lectura e interpretación de los resultados. Actualmente se proponen otros como, solución balanceada de Hank, etanol 70% y Azida de sodio 0,2% (3,11) pero presentan problemas de desinfección, cambios en las propiedades de los especímenes y toxicidad.…”

Section: Introductionunclassified

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Medios de almacenamiento de dientes para estudios de color

2020

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Todos los medios de almacenamiento usados para ensayos de investigación alteran las propiedades físicas y/o químicas de los dientes, especialmente el color dental. El objetivo es establecer cual el medio de almacenamiento más indicado para dientes que van a ser usados en estudios de color basados en una revisión sistematizada, y proponer un protocolo específico. Mediante las palabras clave ¨storage, medium¨, ¨teeth¨, ¨color¨, ¨stability¨, ¨saliva¨, ¨formalin¨, ¨thymol¨, ¨ethanol¨conectadas por AND o OR y consultando las bases de datos PubMed, ScienceDirect, Embase, Scopus, Scielo, Lilacs y Google Scholar, se seleccionaron artículos con nueve criterios de evaluación a tres niveles de evidencia (alto, medio y bajo). Se realizó el análisis, síntesis de la literatura y como producto se establecieron los parámetros para el protocolo de almacenamiento. El resultado final arrojó 8 artículos, dos con nivel de evidencia alto para la saliva natural y/o saliva artificial sin carboximetilcelulosa. Tres con nivel de evidencia medio para el timol 0,1%, cloramina T 0,5%, glutaraldehido 2% y formalina 10%. Tres con nivel de evidencia bajo para el etanol, óxido de etileno o hidróxido de potasio. El medio de almacenamiento más indicado es saliva humana seguida por saliva sintética sin carboximetilcelulosa y se estableció con estos parámetros el protocolo del almacenamiento.

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In vitro polarized Raman analysis for the evaluation of the efficacy of CPP-ACP remineralizing mousse in tooth hypomineralization

Franco

Cardoso-Martins

Arantes-Oliveira

et al. 2021

Results in Chemistry

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Determination of storage solutions influence on human enamel by Raman spectroscopy

Cited by 7 publications

References 21 publications

Effectiveness of Visual-Tactile Examination and DIAGNOdent Pen in Detecting Early Enamel Caries and Its Remineralisation: An In Vitro Study

Effectiveness of Visual-Tactile Examination and DIAGNOdent Pen in Detecting Early Enamel Caries and Its Remineralisation: An In Vitro Study

Medios de almacenamiento de dientes para estudios de color

In vitro polarized Raman analysis for the evaluation of the efficacy of CPP-ACP remineralizing mousse in tooth hypomineralization

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