Determination of Starch in Plant Tissues

Ripe banana fruits were subjected to dip treatment for 4 h using chemicals, plant extracts or their combinations, and shelf life of treated fruits stored at room temperature was monitored by assaying fruit firmness. The treatment of fruit with 1% garlic clove extract or 10% onion bulb extract and adjusting the pH to 4.5 extended the shelf life by 4-5 days when compared with untreated fruit. Treatment with a combination of 1% garlic extract and 1 mm sodium metabisulphite and adjusting the pH to 4.5 prolonged the shelf life of ripe banana to 13-14 days when compared with 6-7 days for the untreated fruit. The treatment delayed the fruit softening by diminishing the rate of starch and pectin degradation. The rate of increase in b-amylase and polygalacturonase activities along with softness was also retarded in treated fruits when compared with control fruits.

show abstract

“…In alcohol‐insoluble fraction, starch was estimated according to the method of Pucher et al. (1948).…”

Section: Methodsmentioning

confidence: 99%

Garlic extract plus sodium metabisulphite enhances shelf life of ripe banana fruit

Sanwal¹,

Payasi

2007

Int J of Food Sci Tech

View full text Add to dashboard Cite

show abstract

“…Starch was extracted with perchloric acid and precipitated with iodine/potassium iodine (I 2 KI) (Pucher et al 1948). Re-dissolved starch was hydrolysed with sulphuric acid and phenol, resulting in an orange solution (Dubois et al 1956).…”

Section: Chemical Analysesmentioning

confidence: 99%

Response to recurrent disturbance in two co-occurring resprouter heath species: the ecological consequences of withstanding herbivores

Paula

Ojeda

2011

Plant Ecol

View full text Add to dashboard Cite

Resprouting woody plants are vulnerable to large mammal herbivores in the early stages of regeneration after disturbance (e.g., fire, slashing), because herbivory reduces the storage of belowground starch reserves and may thus compromise regeneration success. Defence from herbivory may incur a cost to resprouter plants in terms of lengthening the time to recovery of pre-disturbance starch levels. Erica scoparia and E. australis (Ericaceae), two resprouter woody species that co-occur in Mediterranean heath-communities of the Strait of Gibraltar, differ in the way they cope with herbivory: E. scoparia is browsing-tolerant and E. australis is a browsingavoider. Our experimental field study demonstrated that exposure to a moderate density of ungulate herbivores markedly lowered starch re-storage after resprouting in E. scoparia, but not in E. australis. Nevertheless, time to recover pre-disturbance starch levels was shorter in the tolerant E. scoparia, even under exposure to herbivores. The slower starch recovery rate of E. australis is a likely outcome of its resource-demanding browsing-avoidance mechanism. Differences in starch recovery rates after disturbance between the two Erica species partly explains their ecological segregation at the landscape scale.

show abstract

“…Analysis of lipid content and fatty acid composition plant was performed on both the wild-type and egy1-2 hypocotyls according to the method described by Xiao et al (2005). Starch and glucose analysis of hypocotyl segments followed a method described by Pucher et al (1948). Potato starch was used as the positive control.…”

Section: Recombinant Binary Vectors and Transformationmentioning

confidence: 99%

EGY1 plays a role in regulation of endodermal plastid size and number that are involved in ethylene-dependent gravitropism of light-grown Arabidopsis hypocotyls

Guo

Gao

et al. 2007

Plant Mol Biol

View full text Add to dashboard Cite

Egy1 was isolated as an ethylene-dependent gravitropism-deficient Arabidopsis mutant. Molecular studies reveal that EGY1 gene encodes a 59-kDa plastid-targeted metalloprotease. It is actively expressed in hypocotyl tissue and targets to endodermal and cortex plastid. Its protein level is up-regulated by both ethylene and light. CAB protein accumulation and chlorophyll level is severely reduced in hypocotyls and endodermal cells, respectively. Sucrose is able to restore the severely reduced starch and lipid contents as well as the deficient endodermal plastid size found in light-grown egy1 hypocotyls yet it fails to rescue the reduced plastid number and chlorophyll level in egy1 endodermal cells. The loss-of-function egy1 mutation results in a smaller size (1.9 +/- 0.3 microm in diameter) and less number (5 +/- 1) of plastids in endodermal cells, which are nearly 50% of the wild-type. EGY1 is specially required for the development of full-size endodermal plastid in seedlings that are grown on sucrose-free media under light. It plays a direct role in controlling the light-induced chlorophyll production, grana formation and plastid replication in endodermal cell. However, it plays an indirect role in regulation of endodermal plastid size. It is likely that the ethylene-dependent gravitropism-deficient phenotype of egy1 hypocotyls may result from the smaller size and less number of endodermal plastids. Gravicurvature assays performed on ethylene-insensitive mutants, etr1-1, etr2-1, ers2-1, ein4-1 and ein2-5, have clearly demonstrated the necessary role for ethylene in vigorous gravitropism of light-grown hypocotyls. The degree of ethylene-dependent gravicurvature is positively correlated with the combined state of endodermal plastid mass and number. Neither ethylene nor EGY1-regulated full-size endodermal plastid is sufficient for promotion of vigorous hypocotyl gravitropism. Presence of 4 full-size plastids per endodermal cell together with ethylene pretreatment of hypocotyls becomes sufficient to trigger vigorous gravicurvature in light-grown seedlings. A model is therefore proposed to address the role of EGY1 in regulation of endodermal plastid size and number as well as the stimulatory effect of ethylene on hypocotyl gravitropism.

show abstract

Determination of Starch in Plant Tissues

Cited by 54 publications

References 9 publications

Garlic extract plus sodium metabisulphite enhances shelf life of ripe banana fruit

Garlic extract plus sodium metabisulphite enhances shelf life of ripe banana fruit

Response to recurrent disturbance in two co-occurring resprouter heath species: the ecological consequences of withstanding herbivores

EGY1 plays a role in regulation of endodermal plastid size and number that are involved in ethylene-dependent gravitropism of light-grown Arabidopsis hypocotyls

Contact Info

Product

Resources

About