Determination of specific anti-leptospiral immunoglobulins M and G in sera of experimentally infected dogs by solid-phase enzyme-linked immunosorbent assay

Hartman, E.G.; Houten, M. van; Donk, J.A. van der; Frik, J. F.

doi:10.1016/0165-2427(84)90026-6

Cited by 22 publications

(10 citation statements)

References 12 publications

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“…Vaccination titres do not disturb the serodiagnosis of acute leptospirosis because, as a rule, dogs suffering from acute leptospirosis demonstrate a relatively high anti-leptospiral IgM titre together with a low or negative antileptospiral IgG titre (Hartman et al, 1984). Such titres were found only during the time between the first and the booster vaccination (in primarily vaccinated dogs).…”

Section: Discussionmentioning

confidence: 99%

“…Serological examination of the 8 beagles before the primary vaccination experiment demonstrated no agglutination titres equal to or exceeding 20; ELISA IgG titres were<20 and ELISA IgM titres were ~40 in all dogs and should be considered to be negative (Hartman et al, 1984). IgG response.…”

Section: Primary Vaccinationmentioning

confidence: 93%

“…The aim of the present investigation was to determine the production of specific anti-leptospiral IgM and IgG after vaccination of dogs against leptospirosis using the ELISA technique, which has been shown to be a very useful tool for this purpose (Hartman et al, 1984).…”

Section: -2427/84/$0300mentioning

confidence: 99%

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Humoral immune response of dogs after vaccination against leptospirosis measured by an IgM- and IgG-specific ELISA

Hartman

Houten

Frik

et al. 1984

Veterinary Immunology and Immunopathology

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Hartman, E.G., van Houten, M., Frik, J.F. and van der Donk, J.A., 1984. Humoral immune response of dogs after vaccination against leptospirosis measured by an IgM-and IgG-specific ELISA. Vet. Immunol. Immunopathol., 7: 245-254.An IgM-and IgG-specific ELISA was used to measure the antibody response stimulated in dogs by vaccination with a leptospiral bacterin containing chemically inactivated Leptospira interrogans serotype icterohaemorrhagiae and serotype canicola leptospires. All dogs produced anti-leptospiral IgM and IgG. The IgM production was of the primary response type after each vaccination (primary vaccination, booster vaccination and annual revaccination). A substantial antileptospiral IgG response could be demonstrated only after the first booster vaccination and the annual revaccination. Annual revaccination resulted in a higher and much longer persisting IgG response than did the first booster vaccination. A revision of the vaccination scheme is suggested.

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Section: Discussionmentioning

confidence: 99%

Section: Primary Vaccinationmentioning

confidence: 93%

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Humoral immune response of dogs after vaccination against leptospirosis measured by an IgM- and IgG-specific ELISA

Hartman

Houten

Frik

et al. 1984

Veterinary Immunology and Immunopathology

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“…Analyzing reasons of discrepancy between ELISA and MAT results it can be stated that one of them can be higher sensitivity of ELISA (Adler et al 1980, Hartman et al 1984, Ribotta et al 2000). Another reason can be connected with detection by ELISA antibodies specific to other Leptospira serogroups then these, to which belong serovars used in MAT.…”

Section: Discussionmentioning

confidence: 99%

Occurrence of leptospiral infections in swine population in Poland evaluated by ELISA and microscopic agglutination test

Wasiński¹,

Pejsak²

2010

Polish Journal of Veterinary Sciences

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Swine are one of significant reservoirs and sources of Leptospira infections for man. Serological screenings help to effectively control the epidemiological situation in swine herds and to prevent transmission of Leptospira from animals to man. The purpose of this study was to investigate, by the use of serological methods, the prevalence of infections caused by selected Leptospira serogroups in swine population in Poland.A total of 7112 swine serum samples were examined. The samples were collected from January to October 2008 and came from 280 counties situated in all 16 provinces of Poland. All sera were examined preliminary by enzyme-linked immunosorbent assay (ELISA) using heat-stable antigenic preparation. The samples positive or doubtful in ELISA were investigated by microscopic agglutination test (MAT) with use of serovars Icterohaemorrhagiae, Pomona, Canicola, Sejroe, Tarassovi and Grippotyphosa.Of the collected sera examined by ELISA 73 (1.02%) samples were positive, 85 (1.20%) -doubtful and 6954 -negative. Among ELISA-positive and doubtful sera 64 samples (coming from 14 provinces) were recognized in MAT as positive. Among MAT positive samples 42.19% of sera demonstrated titres with serovar Pomona, 32.81% -with Sejroe, 14.06% -with Icterohaemorrhagiae, 6.25% -with Tarassovi, 3.13% -with Grippotyphosa and 1.56% with Canicola.

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“…The test was read on a Dynatech Microelisa Minireader MR590 (wavelength 490 rim). Ig class-specific antibody titres were expressed as the log 2 of i/I0 of the reciprocal of the highest sample dilution giving an optical density of at least 0.2 (Hartman et al, 1984b).…”

Section: Immunization Proceduresmentioning

confidence: 99%

K88 variants K88ab, K88ac and K88ad in oral vaccination of different porcine adhesive phenotypes. Immunological aspects

Bijlsma

Houten

Frik

et al. 1987

Veterinary Immunology and Immunopathology

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Bijlsma, I.G.W., van Houten, M., Frik, J.F., and Ruitenberg, E.J., 1987. K88 variants K88ab, K88ac and K88ad in oral vaccination of different porcine adhesive phenotypes. Immunological aspects. Vet.lmmunol.lmmunopathol.,16: 235-25D.Sows of different adhesive phenotypes were vaccinated orally during the last 4 weeks of gestation with K88-positive Escherichia coli. Sows susceptible to adhesion by the K88 variant of the vaccination strain produced a significant IgA-class specific anti-K88 response in colostrum and milk and post-farrowing serum. Indications for an IgM and IgG-class specific anti-K88 response were also found in this group but only in milk. In sows resistant to adhesion by the K88 variant of the vaccination strain only an IgA-class specific anti-KS8 antibody response was found in mammary secretions and in post-farrowing sera, but titres did not reach the high values of the former group. The response in the second group was attributed to the frequent administration of large quantities of K88-positive E.coli which to some extent can be compared with a colonization effect. Specificity for the serological components of the K88 variants was detectable in colostral IgA of sows susceptible to the vaccination strain only.

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Determination of specific anti-leptospiral immunoglobulins M and G in sera of experimentally infected dogs by solid-phase enzyme-linked immunosorbent assay

Abstract: Hartman, E.G., van Houten, M., van der Donk, J.A. and Frik, J.F., 1984.Determination of specific anti-leptospiral immunoglobulins M and G in sera of experimentally infected dogs by solid-phase enzyme-linked immunosorbent assay. Vet. Immunol. Immunopathol., 7: 43-51.

Cited by 22 publications

References 12 publications

Humoral immune response of dogs after vaccination against leptospirosis measured by an IgM- and IgG-specific ELISA

Humoral immune response of dogs after vaccination against leptospirosis measured by an IgM- and IgG-specific ELISA

Occurrence of leptospiral infections in swine population in Poland evaluated by ELISA and microscopic agglutination test

K88 variants K88ab, K88ac and K88ad in oral vaccination of different porcine adhesive phenotypes. Immunological aspects

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