Determination of specific activities and kinetic constants of biotinidase and lipoamidase in LEW rat and Lactobacillus casei (Shirota)

Hayakawa, Kou; Guo, Lei; Terentyeva, Elena A.; Li, Xiaokang; Kimura, Hiroyuki; Hirano, Masahiko; Yoshikawa, Kazuyuki; Nagamine, Takahiko; Katsumata, Noriyuki; Ogata, Tsutomu; Tanaka, Toshiaki

doi:10.1016/j.jchromb.2006.07.006

Cited by 13 publications

(12 citation statements)

References 19 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…We have previously found that Ebios (dried yeast) contains high amount of bound-form biotin [17] and Lactobacillus casei (Shirota) in Yakult contains high V of bacterial biotinidase [9]. Then, we have hypothesized that simultaneous oral-administration of Ebios and Yakult could liberate free biotin within the gut, and biotin level in serum could be elevated.…”

Section: Discussionmentioning

confidence: 99%

“…Since the molecular weights of these hydrophobic membraneproteins (UHS-KAP, OR, PLXA) and ATX are less than 46,000, application of PDMD method after size-exclusion chromatographic (SEC) separation using non-ionic detergent [9] together with the PECderivatization of serum proteins [3] may become the powerful tool for the prompt biotechnological diagnosis.…”

Section: Discussionmentioning

confidence: 99%

“…Biotinidase (EC 3.5.1.12) activity in the serum was determined by an RP-HPLC method as described previously [9]. Thermal instability test was also performed by incubating the diluted serum by the 0.1 M sodium phosphate buffer (1 mM EDTA, 10% (v/v) glycerol, pH 6.8 [13]) for 4 h at 37°C [18].…”

Section: Biotinidase Activitymentioning

confidence: 99%

See 2 more Smart Citations

Changes in the Hydrophobic Proteins in Response to Biotin Administration in Serum of Infant Patients with Alopecia as Assessed by the Protein Micro-Sequencing Method

Hayakawa¹

2015

J Microb Biochem Technol

View full text Add to dashboard Cite

show abstract

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

Changes in the Hydrophobic Proteins in Response to Biotin Administration in Serum of Infant Patients with Alopecia as Assessed by the Protein Micro-Sequencing Method

Hayakawa¹

2015

J Microb Biochem Technol

View full text Add to dashboard Cite

show abstract

“…Therefore, human serum BIN has now been recognized as the dimer enzyme; i.e., 10-fold higher affinity for biocytin is obtainable in the dimer (Km = 0.48 μM) than a monomer (Km = 5.1 μM) , and we would like to designate this 10-fold increasing phenomenon in the affinity by the association of monomer as Fascio effect (bundle or sheaf in Italian). Therefore, our results in Lewis rats suggest that the demonstrated organ differences in BIN Kms are due to different subunit structures and glycochain structures, although the possibility of genetic differences among organs still remain (Hayakawa et al, 2006). Therefore, observed day-to-day cyclic rhythm of ca.…”

Section: Introductionmentioning

confidence: 68%

The Fascio Effect: Biotin and Sleep

Hayakawa¹,

Nagamine²

2016

IJB

Self Cite

View full text Add to dashboard Cite

An Italian maxim says "I bambini che dormono tanto crescono bene", and the English maxim also says that "All children need to sleep in order to grow and develop". In this review, we would like to demonstrate the truth of this proverb about sleep together with biotin (vitamin H) and lipoic acid (thioctic acid). Recently, we have found that D-biotin regulates the membrane biosynthesis in the cells. Previously, we have found that purified biotin-recycling enzyme biotinidase is also able to hydrolyze enkephalin and neuro-peptides. Thus, biotin state and sleep should be strongly linked to each other. In this report, we have investigated the expression of hormone receptors in some diseases using the new proteomics of protein-direct-microsequencing-deciphering (PDMD) method, and have indicated that having adequate sleep and nutrition is important to for healthy immunological and mental conditions in humans. It is also summarized that human breast milk is superior to bovine milk for human healthy babies to sleep and grow well.

show abstract

“…Biotinidase assay with biotinyl-6-aminoquinoline was superior to biotinyl-4-aminobenzoate, since there were less interfering peaks appeared in the HPLC chromatogram of 6-aminoquinoline than p-aminobenzoic acid. Therefore, lipoyl-6--aminoquinoline was devised as alternate synthesized substrate for lipoamidase activity assay [46][47] . The liberated 6-aminoquinoline was separated by HPLC and measured at an excitation wavelength of 350 nm and an emission wavelength of 550 nm by a fluorescence detector.…”

Section: Fig 1 Working Of Hypothesis Of the Metabolic Fate Of Externmentioning

confidence: 99%

Activity assay of Lipoamidase, an expected modulator of metabolic fate of externally administered lipoic acid

Wang

Motafakkerazad

Matsugo

et al. 2011

Inflammation and Regeneration

View full text Add to dashboard Cite

α-Lipoic acid (LA) is a cofactor functioning in four multienzymes: pyruvate dehydrogenase, α-ketoglutarate dehydrogenase, branched-chain α-keto acid dehydrogenase, and the glycine cleavage enzyme systems. In each enzyme, LA is covalently bound to the ε-amino group of lysine residues. LA has been used as a medicine for treating diabetic condition in Europe and is also currently a popular supplement resource, and thus the metabolic fate after orally intake of LA attracts much attention whether externally administered LA is incorporated into protein bound form because large fraction of administered dose is not fully explained yet. Lipoamidase is the enzyme catalyzing formation and breakage of amide bond between LA and lysine ε-amino groups in the protein and thus is expected to play critical role in LA metabolism and function. In order to know how the enzyme lipoamidase is involved in the fate of LA in the body, a simple assay method is requested for determining lipoamidase activity in tissues. The method using simple HPLC detection of newly synthesized fluorescent substrate, dansyl-α-lipoyllysine, is discussed together with other previously reported methods. Rec.2/26

show abstract

Determination of specific activities and kinetic constants of biotinidase and lipoamidase in LEW rat and Lactobacillus casei (Shirota)

Cited by 13 publications

References 19 publications

Changes in the Hydrophobic Proteins in Response to Biotin Administration in Serum of Infant Patients with Alopecia as Assessed by the Protein Micro-Sequencing Method

Changes in the Hydrophobic Proteins in Response to Biotin Administration in Serum of Infant Patients with Alopecia as Assessed by the Protein Micro-Sequencing Method

The Fascio Effect: Biotin and Sleep

Activity assay of Lipoamidase, an expected modulator of metabolic fate of externally administered lipoic acid

Contact Info

Product

Resources

About