Determination of some polyphenolic compounds from<i>Allium</i>species by HPLC-UV-MS

Pârvu, Marcel; Toiu, Anca; Vlase, Laurian; Parvu, Elena Alina

doi:10.1080/14786410903309484

Cited by 42 publications

(32 citation statements)

References 10 publications

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“…The main ingredients of the MHT are known as follows: alkaloids (e.g., ephedrine and pseudoephedrine) from Ephedrae Herba [11], coumarin (e.g., coumarin, cinnamic acid, and cinnamaldehyde) from Cinnamomi Ramulus [12, 13], triterpene saponins (e.g., glycyrrhizin) and flavonoids (e.g., liquiritin and liquiritigenin) from Glycyrrhizae Radix et Rhizoma [14], cyanoglucosides (e.g., amygdalin) from Armeniacae Semen [11], phenols (e.g., 6-gingerol and 6-shogaol) from Zingiberis Rhizoma Crudus [15], and phenylpropanoids (e.g., ferulic acid) and flavonoids (e.g., kaempferol) from Allii Radix [16]. Among the various ingredients, we try simultaneous determination of the nine components ephedrine HCl, amygdalin, liquiritin apioside, liquiritin, coumarin, cinnamic acid, cinnamaldehyde, glycyrrhizin, and 6-gingerol in MHT water extract by a HPLC–PDA method.…”

Section: Discussionmentioning

confidence: 99%

Ma Huang Tang Suppresses the Production and Expression of Inflammatory Chemokines via Downregulating STAT1 Phosphorylation in HaCaT Keratinocytes

Lim

Seo

Jin

et al. 2016

Evidence-Based Complementary and Alternative Medicine

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Ma huang tang (MHT) is a traditional herbal medicine comprising six medicinal herbs and is used to treat influenza-like illness. However, the effects of MHT on inflammatory skin diseases have not been verified scientifically. We investigated determining the inhibitory effects of MHT against inflammation responses in skin using HaCaT human keratinocyte cells. We found that MHT suppressed production of thymus and activation-regulated chemokine (TARC/CCL17), macrophage-derived chemokine (MDC/CCL22), regulated on activation of normal T-cell expressed and secreted (RANTES/CCL5), and interleukin-8 (IL-8) in tumor necrosis factor-α (TNF-α) and interferon-γ- (IFN-γ-) stimulated HaCaT cells. Consistently, MHT suppressed the mRNA expression of TARC, MDC, RANTES, and IL-8 in TNF-α and IFN-γ-stimulated cells. Additionally, MHT inhibited TNF-α and IFN-γ-stimulated signal transducer and activator of transcription 1 (STAT1) phosphorylation in a dose-dependent manner and nuclear translocation in HaCaT cells. Our finding indicates that MHT inhibits production and expression of inflammatory chemokines in the stimulated keratinocytes by downregulating STAT1 phosphorylation, suggesting that MHT may be a possible therapeutic agent for inflammatory skin diseases.

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Section: Discussionmentioning

confidence: 99%

Ma Huang Tang Suppresses the Production and Expression of Inflammatory Chemokines via Downregulating STAT1 Phosphorylation in HaCaT Keratinocytes

Lim

Seo

Jin

et al. 2016

Evidence-Based Complementary and Alternative Medicine

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“…[18,19,20]. Results of some researchers showed the importance of phenolic compounds in species of the genus Allium [21,22,23]. Evaluation of the biological effects of plant allows a significant contribution to medicinal plant study and their pharmaceutical applications.…”

Section: Introductionmentioning

confidence: 98%

Biological Effects, Total Phenolic Content and Flavonoid Concentrations of Fragrant Yellow Onion (Allium flavum L.)

Ćurčić

Stanković²,

Radojević³

et al. 2012

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The antioxidant, antibacterial and antiproliferative activities, total phenolic content and concentrations of flavonoids of A. flavum extracts were determined. The total phenolic content was determined with Folin-Ciocalteu reagent and it ranged between 42.29 to 80.92 mg GA/g. The concentration of flavonoids in various extracts of A. flavum was determined using spectrophotometric method with aluminum chloride and obtained results varied from 64.07 to 95.71 mg RU/g. The antioxidant activity was monitored spectrophotometrically and expressed in terms of IC50 (μg/ml), and its values ranged from 64.34 to 243.34 μg/ml. The highest phenolic content and capacity to neutralize DPPH radicals were found in acetone extract. Antibacterial efficacy was defined by determining minimum inhibitory and minimum bactericidal concentrations using microdilution method. Significant antibacterial activity, especially for ethyl acetate extract, was observed. The best activity was showed against G+ bacteria, Staphylococcus aureus ATCC 25923 and Bacillus subtilis, while Escherichia coli was one of the least sensitive bacteria. Antiproliferative activity of the methanolic extract on HCT- 116 cell line was determined by MTT assay. Results showed that A. flavum has good antiproliferative activity with IC50 values of 28.29 for 24 h and 35.09 for 72 h. Based on these results, A. flavum is a potential source of phenols as natural antioxidant, antibacterial and anticancer substance of high value. Phenolic content of extracts depend on the solvents used for extraction.

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“…The pharmacological effects are caused by diallyl sulphides (diallyl monosulphide, diallyl disulphide, diallyl trisulphide, diallyl tetrasulphide), polyphenolic compounds, vitamin C and carotenoids (Vlase et al, 2013). In opinion of Parvu et al (2010) the main polyphenolic compounds of chives are p-coumaric acid, ferulic acid, isoquercitrin and rutin.…”

Section: Introductionmentioning

confidence: 99%

Evaluation and comparison of the content of total polyphenols and antioxidant activity of selected species of the genus Allium

Lenková¹,

Bystrická²,

Tóth³

et al. 2016

j. cent. eur. agric.

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The species of the genus Allium are very important crops for human health. They contain many health beneficial substances, such as polyphenols (especially flavonoids), sulphur compounds, vitamins, mineral substances and substances with antioxidant activity. This work has focused on the comparison of total phenolic content and antioxidant activity of selected species of the genus Allium -garlic (Allium sativum L.), chives (Allium schoenoprasum L.), ramson (Allium ursinum L.) and red, yellow and white onion (Allium cepa L.). Samples of plant material were collected at the stage of full maturity in the area of Nitra. Total polyphenols content was determined using the spectrophotometric method of Folin-Ciocalteu agents. Determined the content of total polyphenols were in the range 444.3 -1591 mg*kg -1 . Total polyphenols content in the observed crops declined in the following order: chives > red onion > garlic > yellow onion > ramson > white onion. Antioxidant activity was measured by the spectrophotometric method using a compound DPPH (2.2-diphenyl-1-picryhydrazyl). Determined the value of antioxidant activity ranged 12.29 -76.57%. Antioxidant activity observed in crops declined in the following order: chives > ramson > red onion > yellow onion > garlic > white onion. In all the analysed crop plants was confirmed by the strong dependence of the antioxidant activity and the total content of polyphenolic substances. AbstraktDruhy z rodu Allium sú veľmi dôležité plodiny pre ľudské zdravie. Obsahujú množstvo dôležitých zdraviu prospešných látok, medzi ktoré patria polyfenoly (hlavne flavonoidy), sírne zlúčeniny, vitamíny, minerálne látky a látky s antioxidačnou aktivitou. Táto práca bola zameraná na porovnanie obsahu celkových polyfenolov a antioxidačnej aktivity vo vybraných druhoch z rodu Allium -cesnak (Allium sativum L.), pažítka (Allium schoenoprasum L.), cesnak medvedí (Allium ursinum L.) a červená, žltá a biela cibuľa (Allium cepa L.). Vzorky rastlinného materiálu boli zhromažďované v štádiu plnej zrelosti v lokalite Nitra. Obsah celkových polyfenolov bol stanovený spektrofotometrickou metódou s použitím Folin-Ciocalteuovho činidla. Stanovené obsahy celkových polyfenolov sa pohybovali v rozmedzí 444.3 -1591 mg*kg -1 . Obsah celkových polyfenolov v sledovaných plodinách klesal v nasledujúcom poradí: pažítka > červená cibuľa > cesnak > žltá cibuľa > cesnak medvedí > biela cibuľa. Antioxidačná aktivita bola meraná spektrofotometrickou metódou za použitia zlúčeniny DPPH (2.2-difenyl-1-pikryhydrazyl). Stanovené hodnoty antioxidačnej aktivity sa pohybovali v rozmedzí 12.29 -76.57%. Antioxidačná aktivita v sledovaných plodinách klesala v nasledujúcom poradí: pažítka > cesnak medvedí > červená cibuľa > žltá cibuľa > cesnak > biela cibuľa. Vo všetkých analyzovaných plodinách bola potvrdená silná závislosť medzi antioxidačnou aktivitou a celkovým obsahom polyfenolických látok.

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Determination of some polyphenolic compounds fromAlliumspecies by HPLC-UV-MS

Cited by 42 publications

References 10 publications

Ma Huang Tang Suppresses the Production and Expression of Inflammatory Chemokines via Downregulating STAT1 Phosphorylation in HaCaT Keratinocytes

Ma Huang Tang Suppresses the Production and Expression of Inflammatory Chemokines via Downregulating STAT1 Phosphorylation in HaCaT Keratinocytes

Biological Effects, Total Phenolic Content and Flavonoid Concentrations of Fragrant Yellow Onion (Allium flavum L.)

Evaluation and comparison of the content of total polyphenols and antioxidant activity of selected species of the genus Allium

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