Determination of some doping agents by overpressured thin-layer chromatography

Gulyas, Holger; Kemény, Gábor; Hollósi, I.; Pucsok, József Márton

doi:10.1016/s0021-9673(00)95062-3

Cited by 26 publications

(2 citation statements)

References 10 publications

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“…The OPLC1 system is based on the method of Gulyás et al, 13 which was originally designed for stimulant type doping agents. The system, as such, was tested to be suitable for a broader range of drugs by the present authors but, in the final version, chloroform was replaced by trichloroethylene to obtain more concise analyte fractions.…”

Section: Resultsmentioning

confidence: 99%

Toxicological Drug Screening by Overpressured Layer Chromatography

Ojanperä

Goebel

Vuori

1999

Journal of Liquid Chromatography & Related Technologies

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Two overpressured layer chromatography (OPLC) systems were developed for the screening of toxicologically relevant basic drugs in forensic and clinical contexts. The OPLC1 system was trichloroethylene -methylethylketone -n-butanol -acetic acidwater 17+8+25+6+4 and the OPLC2 system was butyl acetateethanol -tripropylamine -water 85+9.25+5+0.75 with presaturation. Both systems were tested on high performance silica gel plates. The R f values of the drugs were reproducible, the peak shapes were symmetrical, and the chromatographic systems showed low mutual correlation (r = 0.103). The separation numbers (SN) were 27.5 and 29.2 for OPLC1 and OPLC2, respectively, which are more than two times higher than those obtained with TLC systems in general. The combination of the systems was demonstrated to be feasible in the screening for drugs in autopsy urine samples, utilizing automated identification by hR f c /UV library search with combined dual-system reporting. Downloaded by [University of California Santa Cruz] at 13:28 03 December 2014 ORDER REPRINTS 162OJANPERÄ ET AL. ORDER REPRINTS OVERPRESSURED LAYER CHROMATOGRAPHY OF DRUGS 169The most concise fractions, in general, were obtained by using a more lipophilic carbonyl compound, butyl acetate, with a small amount of water and by adjusting the final mobile phase strength with an alcohol. CONCLUSIONSThis study is the first that introduces OPLC methods for comprehensive drug screening, showing that even compounds with large differences in polarity properties can be analyzed by single OPLC methods. However, this requires a very careful adjustment of the mobile phase components to avoid the formation of interfering fronts and to obtain a favorable analyte distribution. The present two methods possess separation numbers over two times higher than ordinary TLC methods and show little mutual correlation.In addition, the developing times are shorter than generally in TLC. Though the current instrumentation allows easy and well controlled operation, its main disadvantage is the relatively high price of factory-sealed OPLC plates.

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Section: Resultsmentioning

confidence: 99%

Toxicological Drug Screening by Overpressured Layer Chromatography

Ojanperä

Goebel

Vuori

1999

Journal of Liquid Chromatography & Related Technologies

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“…Since then, the technique has received even more acceptance, and the number of publications dealing with OPLC in food analysis, the quality control of pharmaceuticals, biomedical analysis and in the analysis of bioactive, plant-derived compounds has increased. Gulyas (1985) has employed OPLC to determine aflatoxins in peanut meal, cornflour and milk powder. Garami and Mincsovics (1989) reported the determination of quaternary ammonium compounds from maize by OPLC.…”

Section: Planar Chromatograpfiymentioning

confidence: 99%

New planar chromatographic techniques in natural product analysis

Erdelmeier

Körig

1991

Phytochemical Analysis

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Planar chromatography (PLC) is gaining importance in the analysis of natural products. Sophisticated instrumentation has become available in this field. Major progress in planar chromatography has been achieved through the development of forced-flow techniques. Progress has also been made in the capillary-controlled flow techniques with the use of modified development chambers. A large variety of stationary phases is available and strategies to optimize mobile phase compositions in PLC have been developed. The detection and quantitation in PLC has improved through direct coupling of PLC with spectroscopic methods with and the use of modern, computerized scanning densitometers.

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Developments of Forensic Toxicological Analysis of Methamphetamine

Frank

Sobol

1990

Forensic Science Progress

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Determination of some doping agents by overpressured thin-layer chromatography

Cited by 26 publications

References 10 publications

Toxicological Drug Screening by Overpressured Layer Chromatography

Toxicological Drug Screening by Overpressured Layer Chromatography

New planar chromatographic techniques in natural product analysis

Developments of Forensic Toxicological Analysis of Methamphetamine

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