Determination of sodium and potassium ions in patients with SARS-Cov-2 disease by ion-selective electrodes based on polyelectrolyte complexes as a pseudo-liquid contact phase

Pershina, Liubov V.; Grabeklis, Andrei R.; Isankina, L. N.; Skorb, Ekaterina V.; Nikolaev, Konstantin G.

doi:10.1039/d1ra04582b

Cited by 5 publications

(3 citation statements)

References 52 publications

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“…Potential measurements between the reference and working electrodes were carried out using a Potentiostat/Galvanostat SP50 (Electrochemical Instruments, Russia) in a standard two-electrode cell at room temperature (23 °C) in a solution of 1 M KCl with Gly.…”

Section: Methodsmentioning

confidence: 99%

Universal Method Based on Layer-by-Layer Assembly for Aptamer-Based Sensors for Small-Molecule Detection

Rukhlyada,

Matytcina,

Baldina

et al. 2023

Langmuir

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Development of a fast and accurate pesticide analysis system is a challenging task, as a large amount of commonly used pesticide has negative effects on humans' health. Detection of pesticide residues is crucial for food safety management and environmental protection. Aptamers�short single-stranded oligonucleotides (RNA or DNA) selected by aptamer selection method SELEX�can selectively bind to their target pesticide molecules with high affinity. Thus, in the present study, we developed an electrochemical biosensor based on aptamers to detect the commonly used pesticide, glyphosate. Carbon fibers were used as the platform to assemble polyelectrolyte layers with the incorporated aptamers selectively binding with glyphosate molecules for electrochemical detection. The best limit of detection of 0.3 μM was achieved at open-circuit potential measurements, which is comparable to the current need in detection of glyphosate. The developed method can be implemented into existing systems for the determination of pesticides on farms to control residual concentrations of glyphosate in soil and water.

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Section: Methodsmentioning

confidence: 99%

Universal Method Based on Layer-by-Layer Assembly for Aptamer-Based Sensors for Small-Molecule Detection

Rukhlyada,

Matytcina,

Baldina

et al. 2023

Langmuir

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“…Ion permeability measurements. The PEI–PSS coacervate membrane was prepared and tested against permeability of K + ions on a K + -selective electrode, fabrication of which is described in detail in [ 42 ]. Briefly, the electrochemical cell was based on carbon fiber and consisted of working (WE) and reference electrodes (RE).…”

Section: Methodsmentioning

confidence: 99%

Periodic Self-Assembly of Poly(ethyleneimine)–poly(4-styrenesulfonate) Complex Coacervate Membranes

et al. 2022

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Coacervation is a self-assembly strategy based on the complexation of polyelectrolytes, which is utilized in biomedicine and agriculture, as well as automotive and textile industries. In this paper, we developed a new approach to the on-demand periodic formation of polyelectrolyte complexes through a Liesegang-type hierarchical organization. Adjustment of reaction conditions allows us to assemble materials with a tunable spatiotemporal geometry and establish materials’ production cycles with a regulated periodicity. The proposed methodology allows the membrane to self-assemble when striving to reach balance and self-heal after exposure to external stimuli, such as potential difference and high pH. Using chronopotentiometry, K+ ion permeability behavior of the PEI–PSS coacervate membranes was demonstrated. The periodically self-assembled polyelectrolyte nanomembranes could further be integrated into novel energy storage devices and intelligent biocompatible membranes for bionics, soft nanorobotics, biosensing, and biocomputing.

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“…Studies have reported that the level of cholinesterase will decrease in the acute stage of severe SARS-CoV-2 infection. Pershina et al [44] constructed a carbon-fiber-based potentiometric biosensor using polyelectrolyte multilayers to detect the ion concentration in the human biofluid of patients with SARS-CoV-2. Polyethyleneimine/polystyrene sulfonate complex has hygroscopicity and can retain ion clusters of inorganic salts, which allows the adhesion of hydrophobic ion selective membrane and produces Nernst response in miniature sensor system.…”

Section: Potentiometric Biosensorsmentioning

confidence: 99%

Perspective Chapter: Novel Diagnostics Methods for SARS-CoV-2

Yang¹,

Li²

2023

Infectious Diseases

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A novel coronavirus of zoonotic origin (SARS-CoV-2) has recently been recognized in patients with acute respiratory disease. COVID-19 causative agent is structurally and genetically similar to SARS and bat SARS-like coronaviruses. The drastic increase in the number of coronavirus and its genome sequence has given us an unprecedented opportunity to perform bioinformatics and genomics analysis on this class of viruses. Clinical tests such as PCR and ELISA for rapid detection of this virus are urgently needed for early identification of infected patients. However, these techniques are expensive and not readily available for point-of-care (POC) applications. Currently, lack of any rapid, available, and reliable POC detection method gives rise to the progression of COVID-19 as a horrible global problem. To solve the negative features of clinical investigation, we provide a brief introduction of the various novel diagnostics methods including SERS, SPR, electrochemical, magnetic detection of SARS-CoV-2. All sensing and biosensing methods based on nanotechnology developed for the determination of various classes of coronaviruses are useful to recognize the newly immerged coronavirus, i.e., SARS-CoV-2. Also, the introduction of sensing and biosensing methods sheds light on the way of designing a proper screening system.

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Determination of sodium and potassium ions in patients with SARS-Cov-2 disease by ion-selective electrodes based on polyelectrolyte complexes as a pseudo-liquid contact phase

Abstract: Supramolecular assemblies based on polyelectrolyte complexes made it possible to create complex interfaces with predictable properties. Polyelectrolyte complexes serve as a pseudo-liquid contact in ion-selective electrodes.

Cited by 5 publications

References 52 publications

Universal Method Based on Layer-by-Layer Assembly for Aptamer-Based Sensors for Small-Molecule Detection

Universal Method Based on Layer-by-Layer Assembly for Aptamer-Based Sensors for Small-Molecule Detection

Periodic Self-Assembly of Poly(ethyleneimine)–poly(4-styrenesulfonate) Complex Coacervate Membranes

Perspective Chapter: Novel Diagnostics Methods for SARS-CoV-2

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