Determination of skin wound age by using cytokines as potential markers

Birincioğlu, İsmail; Akbaba, Murat; Alver, Ahmet; Kül, Seval; Özer, Erdal; Turan, Nurşen; Şentürk, Ayşe; İnce, İmran

doi:10.1016/j.jflm.2016.08.011

Cited by 18 publications

(17 citation statements)

References 43 publications

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“…He commented that it can help as a beneficial method to estimate vitality and aging of wounds, especially during early post‫ـ‬traumatic period before the leukocytic reactions. On another hand this is against what was found by [14] who stated that no wound groups showed a high increase in IL‫1ـ‬b level in comparison to control group. The difference was not significant from statistical view.…”

Section: Discussioncontrasting

confidence: 73%

“…IL-1β copies are tightly regulated, stimulated by stimulation by TIR or IL-1R stimulation (the first signal required to produce IL-1β) As previously described, IL-1β precursors are bisected in its active form by caspase-1, after inflammatory activation with the NLR (NOD like receptor: NOD for nucleotide-binding domain, Lucien rich repeat containing receptors) pathway (signal 2) [6] . The secretion mechanisms of IL-1β and IL-18 in extracellular space are unclear, but proposed pathways include exocytosis of secretory lysosomes, shedding of membrane micro-vesicles or exososomes, or transfer by membrane vectors, such as ASC (alanine serine cysteine amino-acids transport system) transporters [7] .…”

Section: Introductionmentioning

confidence: 99%

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Mast cell, IL-1 Beta and IL-6 for Wound timing and Vitality in Forensic Practice

Jebur¹,

Hashim²,

Al-Obeidi³

2020

IJFMT

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the application of immunohistochemistry has opened a new era for examining the age of the wound by forensic specialists, The aim of our study was to illustrate the use of immunohistochemical marker of mast cells activation (mast cell tryptase "MCT") as a reliable and promising factor of wounds vitality and to Identify the role of pro-inflammatory cytokines: IL 1 beta and IL 6 as parameters of wound age determination.The study was done in the Medico-Legal Directorate of Forensic Medicine (MLD) in Baghdad. The specimens were sera and skin tissues for immunohistochemistry examination, and staining procedures and immunohistochemistry (MCT) ELISA sandwich plate will measure the levels of interleukins. Total autopsy cases were 88 and divided into study group (SG) and control group (CG) .We found that density of Mast cells in sample group was significantly high and higher infiltration that correlates with time passing in the study group as compared by other group in dermis of the sample and control lesions and positive relationship between levels of IL 1‫ـ‬beta & IL‫6ـ‬ level and time progress of wound..

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Section: Discussioncontrasting

confidence: 73%

Section: Introductionmentioning

confidence: 99%

Mast cell, IL-1 Beta and IL-6 for Wound timing and Vitality in Forensic Practice

Jebur¹,

Hashim²,

Al-Obeidi³

2020

IJFMT

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“…At the initial stages of healing, TNF- α plays a key role in the recruitment of inflammatory cells to the site of the lesion [65] and still appears to interfere with type I collagen degradation in normal skin fragments, increasing the collagenolytic activity of MMP-1 [22]. In culture of human fibroblasts, it was demonstrated that TNF- α moderately inhibits both collagen gene transcription and synthesis [66].…”

Section: Discussionmentioning

confidence: 99%

In Situ Cytokine Expression and Morphometric Evaluation of Total Collagen and Collagens Type I and Type III in Keloid Scars

Silva

Tiveron

Silva

et al. 2017

Mediators of Inflammation

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Keloids are characterized by excessive collagen deposition and growth beyond the edges of the initial injury, and cytokines may be related to their formation. The objective of this study was to evaluate the collagen fibers, analyze in situ expression of cytokines in keloid lesions, and compare to the control group. Results showed that there was a predominance of women and nonwhite and direct black ancestry. Keloid showed a significant increase in total and type III collagen. Significantly, the expression of mRNA for TGF-β in keloid was increased, the expressions of IFN-γ, IFN-γR1, and IL-10 were lower, and IFN-γR1 and TNF-α had no statistical difference. Correlations between collagen type III and TGF-β mRNA expression were positive and significant, IFN-γ, IFN-γR1, and IL-10 were negative and significant, and TNF-α showed no statistical difference. We conclude that there was a significant increase of total collagen in keloid and predominance of collagen type III compared to the controls, showing keloid as an immature lesion. There is a significant increase in TGF-β mRNA in keloid lesions, and a significant decrease in IFN-γ and IL-10, suggesting that these cytokines are related to keloid lesions.

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“…Two studies (28,29) used In Situ Labeling of DNA fragments (ISEL) to detect DNA fragments. The other two studies used ELISA (45,47), and the other two used immunofluorescence analysis (51,52). Finally, a study (54) used Cytochemistry analysis, and another study (63) used enzyme histochemical analysis (Table 3).…”

Section: Laboratory Methodsmentioning

confidence: 99%

“…A more recent study ( 47 ) indicates IL-6 and TNF-α as early phase markers indicating a wound age of <30 min. However, they consider that the usefulness of IL-1β and EGF should be reevaluated.…”

Section: Wound Characterization In Post-mortem Forensic Studiesmentioning

confidence: 99%

Forensic Impact of the Omics Science Involved in the Wound: A Systematic Review

et al. 2022

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Background: In forensic autopsies, examining the wounds is one of the most critical aspects to clarify the causal relationship between the cause of death and the wounds observed on the corpse. However, on many occasions, it is difficult to differentiate antemortem injuries from post-mortem injuries, mainly when they occur very close to the moment of death. At present, various studies try to find biomarkers and clarify the molecular mechanisms involved in a wound due to the high variability of conditions in which they occur, thus being one of the most challenging problems in forensic pathology. This review aimed to study the omics data to determine the main lines of investigation emerging in the diagnosis of vital injuries, time of appearance, estimation of the age and vitality of the wound, and its possible contributions to the forensic field.Methods: A systematic review of the human wound concerning forensic science was carried out by following PRISMA guidelines.Results: This study sheds light on the role of omics research during the process of wounding, identifying different cytokines and other inflammatory mediators, as well as cells involved in the specific stage of the wound healing process, show great use in estimating the age of a wound. On the other hand, the expression levels of skin enzymes, proteins, metal ions, and other biomarkers play an essential role in differentiating vital and post-mortem wounds. More recent studies have begun to analyze and quantify mRNA from different genes that encode proteins that participate in the inflammation phase of a wound and miRNAs related to various cellular processes.Conclusions: This study sheds light on the role of research in the molecular characterization of vital wounds, heralding a promising future for molecular characterization of wounds in the field of forensic pathology, opening up an important new area of research.Systematic Review Registration: URL: https://www.crd.york.ac.uk/prospero/#myprospero, Identifier: CRD42021286623.

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Determination of skin wound age by using cytokines as potential markers

Cited by 18 publications

References 43 publications

Mast cell, IL-1 Beta and IL-6 for Wound timing and Vitality in Forensic Practice

Mast cell, IL-1 Beta and IL-6 for Wound timing and Vitality in Forensic Practice

In Situ Cytokine Expression and Morphometric Evaluation of Total Collagen and Collagens Type I and Type III in Keloid Scars

Forensic Impact of the Omics Science Involved in the Wound: A Systematic Review

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