Determination of six polyynes in Oplopanax horridus and Oplopanax elatus using polyethylene glycol modified reversed migration microemulsion electrokinetic chromatography

Abstract

A PEG-modified reversed migration MEEKC method was developed for simultaneous determination of six polyynes, including oplopandiol, falcarindiol, oplopandiol acetate, (11S, 16S, 9Z)-9,17-octadecadiene-12,14-diyne-1,11,16-triol,1-acetate, oplopantriol B, and oplopantriol A, in Oplopanax horridus and Oplopanax elatus. The running buffer containing 0.8% v/v ethyl acetate, 3.8% w/v SDS, 6.6% v/v n-butanol in 20 mM phosphate buffer (pH 2.5), followed by mixing with propan-2-ol at 30% v/v and PEG-1000 at 15% w/v, was applied in the analysis. The proposed method was successfully applied to determine the six polyynes in five samples of Oplopanax horridus and one of O. elatus. The result showed that the types and amounts of polyynes present were obviously different when comparing the two herbs. Besides, the developed PEG-modified reversed MEEKC method might be suitable for the analysis of hydrophobic analytes in herbal medicines.

Oplopanax horridus: Phytochemistry and Pharmacological Diversity and Structure‐Activity Relationship on Anticancer Effects

“…Polyynes have been mostly reported with high contents in both the stem and the root of O. horridus as potential anticancer and antimycobacterial (antituberculosis) natural products. Due to the limited number of analytical chemistry investigations that have been done, only six polyynes have thus far been quantified in the root of O. horridus [ 14 , 15 ]. Phenylpropanoids and lignan glycosides are the other two main natural products from the genus Oplopanax .…”

“…For analytical investigation of polyynes in O. horridus , an HPLC fingerprint method was developed to evaluate the stem and berry extracts of O. horridus , which showed that polyynes are not major components in the stem and berries of O. horridus [ 19 ]. For the quantification of polyynes in O. horridus , an online solid-phase extraction HPLC (SPE-HPLC) and a modified reversed migration microemulsion electrokinetic chromatography were validated to quantify polyynes and one polyene in O. horridus [ 14 , 15 ]. The contents of polyynes in the root bark of O. horridus range from 0.029% to 0.15% ( 1 ), 0.025% to 0.13% ( 2 ), 0.077% to 0.31% ( 3 ), 0.24% to 0.43% ( 4 ), 0.26% to 0.67% ( 5 ), and 0.26% to 0.67% ( 6 ), respectively.…”

Chemical profiling of root bark extract from Oplopanax elatus and its in vitro biotransformation by human intestinal microbiota

“…Polyynes have been found as the main constituents in the root of O. elatus ( Yang et al, 2014 ). Among them, falcarindiol and oplopandiol were determined to have very high contents in the air-dried root bark.…”

Microemulsion Electrokinetic Chromatography in Combination with Chemometric Methods to Evaluate the Holistic Quality Consistency and Predict the Antioxidant Activity of Ixeris sonchifolia (Bunge) Hance Injection

“…From 2000, the Chinese State Food and Drug Administration (SFDA) began to require that all injection preparations made from TCM or their raw materials should be standardized by chromatography fingerprint [ 14 ]. In recent years, MEEKC method was used for determination and separation analytes in complex natural products, such as flavonoids and phenolic acids [ 15 ], polyynes [ 16 ], tobacco alkaloids [ 17 ], curcuminoids [ 18 ] and catechins [ 19 ], but few publications have reported on TCM/herbal preparations fingerprint [ 7 ]. Furthermore, conventional chromatography fingerprint methods are mostly qualitative based on a simple comparison of similarity of the fingerprints, and often lack the quantitative assessment of the fingerprints [ 20 ].…”