Determination of sinigrin and glucoraphanin in Brassica species using a simple extraction method combined with ion-pair HPLC analysis

Rangkadilok, Nuchanart; Nicolas, Marc E.; Bennett, Richard N.; Premier, Robert; Eagling, David R; Taylor, P. W. J.

doi:10.1016/s0304-4238(02)00119-x

Cited by 60 publications

(43 citation statements)

References 33 publications

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“…5B) [45]. Brassica napus, a species resulting from interspecific hybridization between B. rapa and B. oleracea, was reported to be absent in glucoraphanin [63]. This is most likely as result of the AOP2 gene introduced from B. rapa.…”

Section: Discussionmentioning

confidence: 99%

Genome‐wide identification of glucosinolate synthesis genes inBrassica rapa

et al. 2009

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Section: Discussionmentioning

confidence: 99%

Genome‐wide identification of glucosinolate synthesis genes inBrassica rapa

et al. 2009

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“…These compounds have been considered as UV screens in young seedlings (Gitz, Liu, and McClure, 1998) and have been associated with seedling vigour, height and weight (Randhir and Shetty, 2003;Randhir and Shetty, 2005). Regarding B. oleracea seeds, previous studies with different varieties, other than costata, concerned its germination sensitivity to hypoxia (Finch-Savage, Cô me, Lynn, and Corbineau, 2005), the effect of its film-coating to control insect pests (Ester, Putter, and Bilsen, 2003), the determination of glucosinolates (Kaoulla, MacLeod, and Gil, 1980;MacLeod, MacLeod, and Reader, 1989;Rangkadilok et al, 2002aRangkadilok et al, , 2002, fatty acids (Ayaz et al, 2006) and sterols (Matsumoto, Shimizu, Asano, and Itoh, 1983).…”

Section: Introductionmentioning

confidence: 99%

Tronchuda cabbage (Brassica oleracea L. var. costata DC) seeds: Phytochemical characterization and antioxidant potential

et al. 2007

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Tronchuda cabbage (Brassica oleracea L. var. costata DC) seeds were studied for their chemical composition and antioxidant capacity. Thirteen phenolic compounds were characterized and quantified by reversed-phase HPLC-DAD-MS/MS-ESI and HPLC-DAD, respectively: two sinapoylgentiobiose isomers, three sinapoylglucose isomers, kaempferol-3-(sinapoyl)sophorotrioside-7-glucoside, sinapoylcholine, kaempferol-3,7-diglucoside-4 0 -(sinapoyl)glucoside, three disinapoylgentiobiose isomers, 1,2,2 0 -trisinapoylgentiobiose and 1,2-disinapoylglucose. Seven organic acids (aconitic, citric, ascorbic, malic, quinic, shikimic and fumaric acids) were also identified and quantified by HPLC-UV. The aqueous extract of tronchuda cabbage seeds was investigated for its capacity to act as a scavenger of DPPH Å radical and reactive oxygen species (superoxide radical, hydroxyl radical and hypochlorous acid), exhibiting antioxidant capacity in a concentration-dependent manner against all radicals. These results may be attributed to the high content of hydroxycinnamic derivatives and ascorbic acid.

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“…Além disso, o ataque de pragas pode alterar notadamente o nível de glicosinolatos nas folhas (Velasco et al, 2007). Rangkadilok et al (2002b) Tabela 3. Coeficiente de correlação (R) e teste T para R (p ≤ 0,05) entre os métodos utilizados para avaliar o consumo foliar e o dano provocado pelas lagartas de Plutella xylostella em folhas de couve e brócolis com sinigrina.…”

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Consumo foliar da traça-das-crucíferas em couve e brócolis tratados com sinigrina

et al. 2014

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A estimativa da área foliar, em função da sua redução causada pelo consumo por insetos filófagos, é uma metodologia básica em várias áreas da Entomologia, como, por exemplo, em estudos sobre resistência de plantas, entomologia econômica e ecologia nutricional. Um dos fatores que pode interferir na quantidade de área foliar consumida pelo fitófago é a presença de certos químicos nas folhas, como a sinigrina em brassicáceas. Dessa forma, foram aplicadas em folhas de couve e brócolis diversas concentrações de sinigrina (0,2, 0,4, 0,8, 1,6, e 3,2 mg/mL em solução a 5% de Tween20(r)), medindo-se o consumo da área foliar por lagartas de P. xylostella, o qual foi aferido pela porcentagem de redução da área, diferença de peso e escala visual de notas. Os resultados mostraram que as concentrações 0,2, 0,4 e 3,2 mg/mL de sinigrina aumentam o consumo em folhas de couve e diminuem em folhas de brócolis por lagartas de P. xylostella. As metodologias utilizadas proporcionam resultados similares, sendo as estimativas de peso fresco e nota visual mais práticas.

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Determination of sinigrin and glucoraphanin in Brassica species using a simple extraction method combined with ion-pair HPLC analysis

Cited by 60 publications

References 33 publications

Genome‐wide identification of glucosinolate synthesis genes inBrassica rapa

Genome‐wide identification of glucosinolate synthesis genes inBrassica rapa

Tronchuda cabbage (Brassica oleracea L. var. costata DC) seeds: Phytochemical characterization and antioxidant potential

Consumo foliar da traça-das-crucíferas em couve e brócolis tratados com sinigrina

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