Determination of sialic acid in saliva by means of surface-enhanced Raman spectroscopy as a marker in adnexal mass patients: ovarian cancer vs benign cases

Abstract

BackgroundTo demonstrate the use of surface-enhanced Raman spectroscopy (SERS) to determine sialic acid (SA) levels in saliva using silver nanoparticles as substrates, in adnexal mass patients scheduled for surgical intervention to remove invasive masses, with the aim to compare SA levels in benign tumor vs ovarian cancer patients.MethodsQuantification of SA levels was accomplished by measuring their SERS and calibrating with analytical reagent SA. The mean SA concentration in saliva from 37 benign adnexal mass resulted smaller (5.1 mg/dL) than the mean concentration in 15 Ovarium cancer patients (23 mg/dL). The cancer condition was determined by biopsy of the removed adnexal mass. The CA-125 biomarker was also measured. The predictive potential of both biomarkers is discussed, together with the malignity risk index (MRI).ResultsOur results showed a sensitivity/specificity of 80%/100% with a cutoff to distinguish between benign/cancer cases of SA 15.5 mg/dL, as established from a ROC analysis. Our results suggest that SA may be a more useful biomarker than CA-125 to detect ovarian cancer.ConclusionsOur results suggest that the SA levels measured from saliva may be as good predictors as the MRI index for the presence of ovarian cancer in sensitivity/negative predictive value and outperforms it in specificity/positive predictive value.Electronic supplementary materialThe online version of this article (10.1186/s13048-018-0433-9) contains supplementary material, which is available to authorized users.

Vibrational Spectroscopy Saliva Profiling as Biometric Tool for Disease Diagnostics: A Systematic Literature Review

“…In addition, the size effect has more impact below n = 20 as the sensitivity and specificity values were very random, although the essential statistical parameters were considered in the evaluation for clinical utility. Consequently, other vs. studies using saliva, were not included in this review, due to a number of patients less than 20 per group, and concerning other diseases, such as burning mouth syndrome [ 41 ], asthma [ 42 ], Alzheimer’s disease [ 43 ], chronic renal failure [ 44 ], ovarian cancer [ 45 ], various infections, e.g., influenza [ 46 ] or pseudomonas [ 47 ], and cystic fibrosis [ 48 ]. Therefore, out of the 267 studies, only 18 satisfied the criteria according to the PRISMA guidelines.…”

Determination of sialic acid levels by using surface‐enhanced Raman spectroscopy in periodontitis and gingivitis

“…The Shapiro-Wilk test was used to assess normality, and a Bonferroni and Tukey multiple comparison tests were used to compare the three groups. Receiver operating characteristic (ROC) curves were plotted to calculate the area under the curve and establish the threshold SA level that provides optimized values for sensitivity and specificity (Zermeño-Nava et al, 2018). The values of age and oral parameters for the three study groups are expressed as mean and corresponding standard deviations (SD), summarized in Table 1. Before starting the study, the clinical examiner was supervised in the diagnosis of periodontitis by an expert.…”

Photonics of human saliva: potential optical methods for the screening of abnormal health conditions and infections

“…Spectra of Salivary Exosomes 2019 [Dekel et al 2019 ] 100% 12 SERS Liquid biopsy for various cancers. 2019 [Zhang et al 2019 ] miRNAs in liver cancer L.O.D 10fM 13 SERS Ovarian cancer 2018 [(de Jesús Zermeño-Nava et al 2018 ] 80% 14 Fluorescence Oral pre-cancer 2018 [Kumar et al 2018 ] OSCC to normal 91%, OSMF to normal 92% 15 LIF salivary proteins labelled with Cy3 fluorescent dye and detected by fluorescence 2018 [Liu et al 2018 ] 60% 16 UV-Visible Absorption Saliva in leukaemia 2018 [Joudah et al 2018 ] _ 17 UV- Fluorescence Steady and excited state kinetics; normal, oral premalignant and malignant subjects 2018 [Yuvaraj et al 2018 ] 86.6% 18 SERS Sialic acid (SA) in saliva; breast cancer Detection. 2017 [Hernández-Arteaga et al 2017 ] 94% 19 Spectrophotometry Salivary levels of zinc, iron and copper in Head and Neck Cancer 2017 [George et al 2017 ] _ 20 SERS Nasopharyngeal carcinoma NPC.…”