Determination of seven selected neuro- and immunomodulatory steroids in human cerebrospinal fluid and plasma using LC-MS/MS

Sosvorova, Lucie; Vítků, Jana; Chlupáčová, Tereza; Mohapl, M; Hampl, Richard

doi:10.1016/j.steroids.2015.01.019

Cited by 51 publications

(42 citation statements)

References 48 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Whole-blood plasma was analysed for levels of E 2 , DHT, testosterone and progesterone at the Institute of Endocrinology (Prague, Czech Republic). For the detection of progesterone and testosterone, the protocol for liquid chromatography-tandem mass spectrometry (LC-MS/MS) after Sosvorova et al (2015) was used. However, the protocol for LC-MS/MS following Vitku et al (2015) was applied to measure E 2 .…”

Section: Hormone Level Validationmentioning

confidence: 99%

Ovarian control of growth and sexual size dimorphism in a male-larger gecko

Kubička

Schořálková

Červenka

et al. 2016

Journal of Experimental Biology

View full text Add to dashboard Cite

Sexual size dimorphism (SSD) reflects sex-specific solutions to the allocation of energy among growth, reproduction and survival; however, the proximate mechanisms behind these solutions are still poorly known even in vertebrates. In squamates, sexual differences in body size used to be attributed to direct energy allocation to energetically demanding processes, largely to reproduction. In addition, SSD is assumed to be controlled by specific endogenous mechanisms regulating growth in a sex-specific manner, namely masculinization by male gonadal androgens or feminization by ovarian hormones. We performed a manipulative growth experiment in females of the male-larger gecko Paroedura picta in order to test the reproductive cost hypothesis, the male androgen hypothesis and the ovarian hormone hypothesis. Specifically, we investigated the effect of total ovariectomy, prepubertal ovariectomy, unilateral ovariectomy, and total ovariectomy followed by exogenous estradiol, dihydrotestosterone or testosterone treatment, on female growth in comparison to males and reproductively active females. The present results and the results of our previous experiments do not support the hypotheses that SSD reflects direct energy allocation to reproduction and that male gonadal androgens are involved. However, all lines of evidence, particularly the comparable growth of reproducing intact and unilaterally ovariectomized females, were concordant with the control of SSD by ovarian hormones. We suggest that feminization of growth by female gonadal hormones should be taken into consideration as an endogenous pathway responsible for the ontogeny of SSD in squamates.

show abstract

Section: Hormone Level Validationmentioning

confidence: 99%

Ovarian control of growth and sexual size dimorphism in a male-larger gecko

Kubička

Schořálková

Červenka

et al. 2016

Journal of Experimental Biology

View full text Add to dashboard Cite

show abstract

“…[23,24] In some of these processes, the 7-oxo-DHEA is also identified. [16,20,25] Monitoring of the metabolism of DHEA and its derivatives in mammals would be difficult due to their very low (~ng/mL) concentration, [26] rapid conversion to hormones or inactive conjugates, and a high content of other lipid compounds in the environment. So far, in body fluids of mammals, there were identified only a few metabolites of 7-hydroxy-DHEA containing several hydroxyl groups in their structure.…”

Section: Biohydroxylation Of 7-oxo-dhea a Natural Metabolite Of Dheamentioning

confidence: 99%

Biohydroxylation of 7‐oxo‐DHEA, a natural metabolite of DHEA, resulting in formation of new metabolites of potential pharmaceutical interest

2016

View full text Add to dashboard Cite

Metabolism of steroids in healthy and unhealthy human organs is the subject of extensive clinical and biomedical studies. For this kind of investigations, it is essential that the reference samples of new derivatives of natural, physiologically active steroids (especially those difficult to achieve in the chemical synthesis) become available. This study demonstrated for the first time transformation of 7-oxo-DHEA-a natural metabolite of DHEA, using Syncephalastrum racemosum cells. The single-pulse fermentation of substrate produced two new hydroxy metabolites: 1β,3β-dihydroxy-androst-5-en-7,17-dione and 3β,12β-dihydroxy-androst-5-en-7,17-dione, along with the earlier reported 3β,9α-dihydroxy-androst-5-en-7,17-dione and 3β,17β-dihydroxy-androst-5-en-7-one. Simultaneously, the same metabolites, together with small quantities of 7α- and 7β-hydroxy-DHEA, as well as the products of their reduction at the C-17 were obtained after transformation of DHEA under pulse-feeding of the substrate. The observed reactions suggested that this micro-organism contains enzymes exhibiting similar activity to those present in human cells. Thus, the resulting compounds can be considered as potential components of the eukaryotic, including human, metabolome.

show abstract

“…Even though drug concentrations in brain tissue are difficult to measure, cerebral spinal fluid (CSF) concentrations are considered an acceptable surrogate for brain levels of many compounds . Therefore, during the past several years there have been many methods developed to measure analytes in CSF . Since CSF is a biological fluid that is difficult to obtain in large volumes, a surrogate is often used for preparing calibration curves and quality control (QC) samples.…”

Section: Concentration Of Various Substances In Human Csf Plasma Andmentioning

confidence: 99%

Development of a surrogate matrix for cerebral spinal fluid for liquid chromatography/mass spectrometry based analytical methods

Hooshfar

Basiri

Bartlett

2016

Rapid Comm Mass Spectrometry

View full text Add to dashboard Cite

The percent difference in response ratio has been established to demonstrate how different compounds behave between CSF, aCSF and dilute plasma. Although among the compounds tested some of them showed a very similar MS response in actual and aCSF, there were analytes that demonstrated significant differences in ESI-MS signal when sprayed from these two matrices. However, even in such cases, 1:200 diluted plasma generated results with no significant difference from CSF. Therefore, we recommend that in order to develop robust and dependable bioanalytical LC/MS methods from CSF samples, it is more appropriate to prepare calibration curves and quality control samples in diluted plasma instead of aCSF. Copyright © 2016 John Wiley & Sons, Ltd.

show abstract

Determination of seven selected neuro- and immunomodulatory steroids in human cerebrospinal fluid and plasma using LC-MS/MS

Cited by 51 publications

References 48 publications

Ovarian control of growth and sexual size dimorphism in a male-larger gecko

Ovarian control of growth and sexual size dimorphism in a male-larger gecko

Biohydroxylation of 7‐oxo‐DHEA, a natural metabolite of DHEA, resulting in formation of new metabolites of potential pharmaceutical interest

Development of a surrogate matrix for cerebral spinal fluid for liquid chromatography/mass spectrometry based analytical methods

Contact Info

Product

Resources

About