Determination of Serum Glucose by Isotope Dilution Mass Spectrometry

Abstract

An accurate and precise method for the determination of serum glucose by isotope dilution mass spectrometry was studied for use in establishing the accuracy of glucose methods in clinical laboratories. After adding uniformly labeled [13C6} glucose to the serum, glucose was separated from the serum matrix and converted into a-D-glucofranose cyclic 1,2:3,5-bis(n-butylboronate)-6-acetate (glucose-BBA). Measurements were performed with combined capillary gas chromatography-mass spectrometry. The ratios of the peak areas at m/z 297 and 303 for samples and the calibration mixtures were used for quantification. Analytical results of the SRM serum showed satisfactory agreement with the certified value and the relative standard deviation of the replicates was within 0.5% for all of the serum samples tested.